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Determination of pH in Human Erythrocytes. Sources of Systematic Error

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Published/Copyright: June 1, 2005
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 36 Issue 10

Abstract

The results of a simultaneous determination of the pH value in erythrocytes with the potentiometric measuring method and the 14C-labelled 5,5-dimethyl-2,4-oxazolidinedione (DMO) method showed a mean method difference of 0.026 pH units. The cause of this discrepancy was assumed to be matrix-inherent liquid-junction potentials in the potentiometric measurement. Taking these into account in the calculation leads to consistent values for the methods investigated. The DMO method proved to be free of systematic errors. Another indication of this is that its mean ratio of extracellular to intracellular H+ ion concentration (He+/Hi+) substantially agreed with the distribution ratios of other freely diffusible ions and their pH dependence.

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Published Online: 2005-06-01
Published in Print: 1998-10-13

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Kinetic Study of Apo B100 Containing Lipoprotein Metabolism Using Amino Acids Labeled with Stable Isotopes: Methodological Aspects
  2. Activated Immune System in Patients with Huntington's Disease
  3. Elevated Group 11 Phospholipase A2 Mass Concentration in Serum and Colonic Mucosa in Crohn's Disease
  4. Determination of pH in Human Erythrocytes. Sources of Systematic Error
  5. Rapid Molecular Diagnosis of Erythropoietic Protoporphyria among Swiss Patients
  6. Biochemical Evaluation of Oxidative Stress in Propylthiouracil Treated Hyperthyroid Patients. Effects of Vitamin C Supplementation
  7. Effect of Aminoguanidine on Erythrocyte Lipid Peroxiclation and Activities of Antioxidant Enzymes in Experimental Diabetes
  8. Normalizing Intermethod Free Triiodothyronine Patient Results: Need for Commutable Materials
  9. Evaluation of a Predictive Kinetic Procedure for the Enzymatic Measurement of Creatinine in Serum
  10. Pre-Evaluation and System Optimization of the Elecsys® Thyroid Electrochemiluminescence Immunoassays
  11. Validation of an Automated Enzyme Immunoassay for Interleukin-6 for Routine Clinical Use
  12. First Experience with the REMEDi HS Urine Benzodiazepine Assay
  13. Rapid Variations of the Free Prostate-Specific Antigen Fraction in Serum
  14. Are the Preanalytical Factors Underestimated in Clinical Studies?
  15. Book Review
https://doi.org/10.1515/CCLM.1998.134

Articles in the same Issue

  1. Kinetic Study of Apo B100 Containing Lipoprotein Metabolism Using Amino Acids Labeled with Stable Isotopes: Methodological Aspects
  2. Activated Immune System in Patients with Huntington's Disease
  3. Elevated Group 11 Phospholipase A2 Mass Concentration in Serum and Colonic Mucosa in Crohn's Disease
  4. Determination of pH in Human Erythrocytes. Sources of Systematic Error
  5. Rapid Molecular Diagnosis of Erythropoietic Protoporphyria among Swiss Patients
  6. Biochemical Evaluation of Oxidative Stress in Propylthiouracil Treated Hyperthyroid Patients. Effects of Vitamin C Supplementation
  7. Effect of Aminoguanidine on Erythrocyte Lipid Peroxiclation and Activities of Antioxidant Enzymes in Experimental Diabetes
  8. Normalizing Intermethod Free Triiodothyronine Patient Results: Need for Commutable Materials
  9. Evaluation of a Predictive Kinetic Procedure for the Enzymatic Measurement of Creatinine in Serum
  10. Pre-Evaluation and System Optimization of the Elecsys® Thyroid Electrochemiluminescence Immunoassays
  11. Validation of an Automated Enzyme Immunoassay for Interleukin-6 for Routine Clinical Use
  12. First Experience with the REMEDi HS Urine Benzodiazepine Assay
  13. Rapid Variations of the Free Prostate-Specific Antigen Fraction in Serum
  14. Are the Preanalytical Factors Underestimated in Clinical Studies?
  15. Book Review
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