Antigenic Definition of Cardiac Troponin I
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Catherine Larue
Abstract
The presence of cardiac troponin I in the serum is now considered as one of the most specific biochemical markers of acute myocardial infarction. To improve the knowledge of the antigenic properties of cardiac Troponin I, a set of monoclonal antibodies and polyclonal antibodies against human cardiac troponin I has been tested with overlapping peptides covering the cardiac troponin I sequence. The results indicate that N-terminal and C-terminal cardiac troponin I regions were most often recognized by poly- and monoclonal antibodies. These observations are valuable for choosing the best combination of monoclonal antibodies to set up new immunoassays to detect serum cardiac troponin I earlier after myocardial damage, to understand better which forms are released, and finally to propose appropriate cardiac troponin I standards.
Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- New Trends in Immunoassay Methodology
- Progress in Immunoassay Technology
- Standardization of Steroid Immunoassays – In Theory an Easy Task
- Thermodynamic Parameters in Immunoassay
- Engineering of an Anti-Steroid Antibody: Amino Acid Substitutions Change Antibody Fine Specificity from Cortisol Estradiol
- Antigenic Definition of Cardiac Troponin I
- Peptide Models of Immunological Recognition: Paratope Dissection by Multiple Peptide Synthesis
- Remodeling of Glycoprotein and Carbohydrate Antigens
- Strongly Enhanced Serum Levels of Vascular Endothelial Growth Factor (VEGF) after Poly-trauma and Burn
- Rapid Electrophoretic Separation of Pre-ß-Migrating High Density Lipoproteins Using Automated PhastSystem(tm): Application to Analysis of Lecitihin: Cholesterol Acyltransferase-Deficient Plasma
- Decrease in High Density Lipoprotein Cholesterol (HDL-C) Levels Following Gemfibrozil Therapy
- Reference Intervals for Serum Cystatin C and Serum Creatinine in Adults
- Concepts for a Model of Good Medical Laboratory Services
- Diagnostic Kits Derived from Standard Method "DGKC 94" as a Potential Tool for Improvement of Analytical Standardization and Clinical Utility of Alkaline Phosphatase
- Antioxidant Interferences in Superoxide Dismutase Activity Methods Using Superoxide Radical as Substrate
- Cytokines and Soluble Adhesion Molecules in Laboratory Diagnostics. Report from a Meeting Held at the German Cancer Research Center in Heidelberg on 25 November 1997
- IFCC-WorldLabNews
