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Standardization of Steroid Immunoassays – In Theory an Easy Task
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Linda M. Thienpont
Published/Copyright:
June 1, 2005
Abstract
I deal with the process of standardization of steroid immunoassays on the basis of metrological traceability (SI units). First, I discuss the role theoretically attributed to the calibration of the measurement procedure. Since immunoassays show matrix sensitivity, I associate traceability of calibration with traceability of results pertaining to individual patient samples. From this, I stress that optimization of the immunoassays in terms of specificity and insensitivity to different kinds of interference is necessary before standardization, and that split-sample measurements should be included in the standardization process. Last but not least, I give an overview of the current status of standardization of steroid immunoassays.
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Published Online: 2005-06-01
Published in Print: 1998-06-01
Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- New Trends in Immunoassay Methodology
- Progress in Immunoassay Technology
- Standardization of Steroid Immunoassays – In Theory an Easy Task
- Thermodynamic Parameters in Immunoassay
- Engineering of an Anti-Steroid Antibody: Amino Acid Substitutions Change Antibody Fine Specificity from Cortisol Estradiol
- Antigenic Definition of Cardiac Troponin I
- Peptide Models of Immunological Recognition: Paratope Dissection by Multiple Peptide Synthesis
- Remodeling of Glycoprotein and Carbohydrate Antigens
- Strongly Enhanced Serum Levels of Vascular Endothelial Growth Factor (VEGF) after Poly-trauma and Burn
- Rapid Electrophoretic Separation of Pre-ß-Migrating High Density Lipoproteins Using Automated PhastSystem(tm): Application to Analysis of Lecitihin: Cholesterol Acyltransferase-Deficient Plasma
- Decrease in High Density Lipoprotein Cholesterol (HDL-C) Levels Following Gemfibrozil Therapy
- Reference Intervals for Serum Cystatin C and Serum Creatinine in Adults
- Concepts for a Model of Good Medical Laboratory Services
- Diagnostic Kits Derived from Standard Method "DGKC 94" as a Potential Tool for Improvement of Analytical Standardization and Clinical Utility of Alkaline Phosphatase
- Antioxidant Interferences in Superoxide Dismutase Activity Methods Using Superoxide Radical as Substrate
- Cytokines and Soluble Adhesion Molecules in Laboratory Diagnostics. Report from a Meeting Held at the German Cancer Research Center in Heidelberg on 25 November 1997
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