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Engineering of an Anti-Steroid Antibody: Amino Acid Substitutions Change Antibody Fine Specificity from Cortisol Estradiol
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Patrick Chames
Published/Copyright:
June 1, 2005
Abstract
Immunoassays are widely used for determination of the concentration of steroid hormones. However, obtaining specific anti-steroid monoclonal antibodies remains difficult. We used antibody engineering and phage display methods to change the specificity of an anti-cortisol monoclonal antibody towards estradiol. This work demonstrates that production of recombinant antibodies may be a valuable way of obtaining the high-specificity antibodies required for steroid immunoassays.
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Published Online: 2005-06-01
Published in Print: 1998-06-01
Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- New Trends in Immunoassay Methodology
- Progress in Immunoassay Technology
- Standardization of Steroid Immunoassays – In Theory an Easy Task
- Thermodynamic Parameters in Immunoassay
- Engineering of an Anti-Steroid Antibody: Amino Acid Substitutions Change Antibody Fine Specificity from Cortisol Estradiol
- Antigenic Definition of Cardiac Troponin I
- Peptide Models of Immunological Recognition: Paratope Dissection by Multiple Peptide Synthesis
- Remodeling of Glycoprotein and Carbohydrate Antigens
- Strongly Enhanced Serum Levels of Vascular Endothelial Growth Factor (VEGF) after Poly-trauma and Burn
- Rapid Electrophoretic Separation of Pre-ß-Migrating High Density Lipoproteins Using Automated PhastSystem(tm): Application to Analysis of Lecitihin: Cholesterol Acyltransferase-Deficient Plasma
- Decrease in High Density Lipoprotein Cholesterol (HDL-C) Levels Following Gemfibrozil Therapy
- Reference Intervals for Serum Cystatin C and Serum Creatinine in Adults
- Concepts for a Model of Good Medical Laboratory Services
- Diagnostic Kits Derived from Standard Method "DGKC 94" as a Potential Tool for Improvement of Analytical Standardization and Clinical Utility of Alkaline Phosphatase
- Antioxidant Interferences in Superoxide Dismutase Activity Methods Using Superoxide Radical as Substrate
- Cytokines and Soluble Adhesion Molecules in Laboratory Diagnostics. Report from a Meeting Held at the German Cancer Research Center in Heidelberg on 25 November 1997
- IFCC-WorldLabNews
