Effect of enzymatic digestion on thallus degradation and extraction of hydrosoluble compounds from Grateloupia turuturu
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Claire Denis
Abstract
The extraction of metabolites of industrial interest from seaweed tissue is difficult due to the presence of anionic polysaccharides in the cell walls. We studied enzymatic digestion of Grateloupia turuturu (Rhodophyta) to facilitate extraction of the desired metabolites from this seaweed. Four polysaccharidases (Onozuka R-10 cellulase, agarase, κ- and ι-carrageenases) were used alone and in combination. We studied the efficiency of the enzymatic process in degrading the seaweed thallus; we also investigated the effect of the enzymatic treatment on solubilization of R-phycoerythrin and reducing sugars. The best degradation conditions for Grateloupia thalli were obtained with cellulase alone (0.48 U ml-1), or with a combination of cellulase/κ-carrageenase. However, only the combination cellulase (0.08 U ml-1)/κ-carrageenase (0.01 U ml-1), or the individual enzymes [agarase or ι-carrageenase at low concentration (0.55 U ml-1 and 2.5×10-3 U ml-1)] improved solubilization of R-phycoerythrin compared to the control conditions. The simultaneous use of polysaccharidases enabled solubilization of a large quantity of reducing sugars (40 mg glu g-1 dw). Therefore, this condition appears suitable for the production of oligosaccharides. This result also confirms indirectly the presence of agar, cellulose, κ- and ι-carrageenans in the cell wall of Grateloupia turuturu.
©2009 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Editorial note
- To Professor H.B.S. Womersley in honour of his continued contributions to phycology
- Research articles
- Long-term changes in macroalgal assemblages after increased sedimentation and turbidity in Western Port, Victoria, Australia
- Biology and taxonomic identity of Erythroglossumlusitanicum (Delesseriaceae, Rhodophyta) from the Iberian Peninsula
- Records of Rhodophyta new to the flora of the Iberian Atlantic coast
- Notes on the benthic marine algae of Puerto Rico. IX. Additions to the flora including reports of three species for the first time in the Atlantic Ocean
- Lomentaria benahoarensis (Lomentariaceae, Rhodophyta), a diminutive epiphytic new species from La Palma, Canary Islands (eastern Atlantic Ocean)
- Morphology and anatomy of Halymenia actinophysa (Halymeniales, Rhodophyta) from the southwestern Gulf of California, Mexico
- Typification of Antithamnion nipponicum Yamada et Inagaki (Antithamnieae, Ceramioideae, Ceramiaceae, Ceramiales, Rhodophyta)
- Effect of enzymatic digestion on thallus degradation and extraction of hydrosoluble compounds from Grateloupia turuturu
- Antioxidant and cytotoxic activities of some red algae (Rhodophyta) from Brittany coasts (France)
- Short communication
- Comparison of different procedures for the extraction and partial purification of R-phycoerythrin from the red macroalga Grateloupia turuturu
- Meetings
- Meetings
Articles in the same Issue
- Editorial note
- To Professor H.B.S. Womersley in honour of his continued contributions to phycology
- Research articles
- Long-term changes in macroalgal assemblages after increased sedimentation and turbidity in Western Port, Victoria, Australia
- Biology and taxonomic identity of Erythroglossumlusitanicum (Delesseriaceae, Rhodophyta) from the Iberian Peninsula
- Records of Rhodophyta new to the flora of the Iberian Atlantic coast
- Notes on the benthic marine algae of Puerto Rico. IX. Additions to the flora including reports of three species for the first time in the Atlantic Ocean
- Lomentaria benahoarensis (Lomentariaceae, Rhodophyta), a diminutive epiphytic new species from La Palma, Canary Islands (eastern Atlantic Ocean)
- Morphology and anatomy of Halymenia actinophysa (Halymeniales, Rhodophyta) from the southwestern Gulf of California, Mexico
- Typification of Antithamnion nipponicum Yamada et Inagaki (Antithamnieae, Ceramioideae, Ceramiaceae, Ceramiales, Rhodophyta)
- Effect of enzymatic digestion on thallus degradation and extraction of hydrosoluble compounds from Grateloupia turuturu
- Antioxidant and cytotoxic activities of some red algae (Rhodophyta) from Brittany coasts (France)
- Short communication
- Comparison of different procedures for the extraction and partial purification of R-phycoerythrin from the red macroalga Grateloupia turuturu
- Meetings
- Meetings
