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Comparison of different procedures for the extraction and partial purification of R-phycoerythrin from the red macroalga Grateloupia turuturu
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Claire Denis
Published/Copyright:
February 27, 2009
Abstract
With the aim of solubilizing R-phycoerythrin from Grateloupia turuturu, we tested the efficiency of two extraction solutions (distilled water and phosphate buffer) on fresh and freeze-dried seaweeds. Three different methods enabling a partial purification of this pigment were also tested: ammonium sulfate precipitation, separation in aqueous two-phase systems, and ultrafiltration. The best extraction and partial purification conditions for R-phycoerythrin were observed from freeze-dried algae after application of the precipitation procedure (80% ammonium sulfate). This latter protocol appears to be efficient for separating R-phycoerythrin without denaturation from most proteins and other components (such as sugars) that limit the purification of phycobiliproteins.
Keywords: ammonium sulfate precipitation; aqueous two-phase systems; Grateloupia turuturu; R-phycoerythrin purification; ultrafiltration
Received: 2008-9-9
Accepted: 2008-12-8
Published Online: 2009-02-27
Published in Print: 2009-06-01
©2009 by Walter de Gruyter Berlin New York
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Keywords for this article
ammonium sulfate precipitation;
aqueous two-phase systems;
Grateloupia turuturu;
R-phycoerythrin purification;
ultrafiltration
Articles in the same Issue
- Editorial note
- To Professor H.B.S. Womersley in honour of his continued contributions to phycology
- Research articles
- Long-term changes in macroalgal assemblages after increased sedimentation and turbidity in Western Port, Victoria, Australia
- Biology and taxonomic identity of Erythroglossumlusitanicum (Delesseriaceae, Rhodophyta) from the Iberian Peninsula
- Records of Rhodophyta new to the flora of the Iberian Atlantic coast
- Notes on the benthic marine algae of Puerto Rico. IX. Additions to the flora including reports of three species for the first time in the Atlantic Ocean
- Lomentaria benahoarensis (Lomentariaceae, Rhodophyta), a diminutive epiphytic new species from La Palma, Canary Islands (eastern Atlantic Ocean)
- Morphology and anatomy of Halymenia actinophysa (Halymeniales, Rhodophyta) from the southwestern Gulf of California, Mexico
- Typification of Antithamnion nipponicum Yamada et Inagaki (Antithamnieae, Ceramioideae, Ceramiaceae, Ceramiales, Rhodophyta)
- Effect of enzymatic digestion on thallus degradation and extraction of hydrosoluble compounds from Grateloupia turuturu
- Antioxidant and cytotoxic activities of some red algae (Rhodophyta) from Brittany coasts (France)
- Short communication
- Comparison of different procedures for the extraction and partial purification of R-phycoerythrin from the red macroalga Grateloupia turuturu
- Meetings
- Meetings
