Carrageenan of Eucheuma isiforme (Solieriaceae, Rhodophyta) from Yucatán, Mexico. I. Effect of extraction conditions
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Yolanda Freile-Pelegrín
Abstract
The yield and physicochemical properties of carrageenan from Eucheuma isiforme harvested at the Yucatán coast were investigated. Carrageenan was extracted under different alkali concentrations (0, 1, 3, 5 and 7% KOH) and treatment durations (3, 4 and 5 h). Native carrageenan, extracted without KOH, had the highest yield (∼44.6%) independently of treatment duration. After alkali treatment, carrageenan yield ranged from 35.3 to 31.8%. No significant differences in carrageenan yield were observed between 1 and 3% KOH. Native carrageenan had low viscosity values (39–57.0 cPs), whereas carrageenan extracted with 1% KOH at 3 and 4 h increased in viscosity (160.0–161.3 cPs). Alkali-treated carrageenan formed very weak gels (<50 g cm-2) in 1.5% solutions. The chemical analysis and FTIR spectra revealed a preponderantly iota-carrageenan extract. Extractions performed with 1% KOH for 3 h produced carrageenan with suitable properties to be considered as a substitute for traditional iota-carrageenan sources.
References
Anderson, N.S., T.C.S. Dolan and D.A. Rees. 1973. Carrageenans. Part VII. Polysaccharides from Eucheuma spinosum and Eucheuma cottonii. The covalent structure of ι-carrageenan. J. Chem. Soc. Perkin Trans.1: 2173–2176.10.1039/P19730002173Search in Google Scholar
Ask, E., A. Batibasaga, J.M. Zertuche-González and M. de San. 2003. Three decades of Kappaphycus alvarezii (Rhodophyta) introduction to non-endemic location. Proc. Int. Seaweed Symp.17: 49–57.Search in Google Scholar
Azanza-Corrales, R. and P. Sa-a. 1990. The farmed Eucheuma species (Gigartinales, Rhodophyta) in Danajon Reef, Philippines: carrageenan properties. Hydrobiologia204/205: 521–525.Search in Google Scholar
Bellion, C., G.K. Hamer and W. Yaphe. 1981. Analysis of kappa-iota hybrid carrageenans with kappa-carrageenase, iota-carrageenase and 13C-NMR. Proc. Int. Seaweed Symp.10: 379–384.Search in Google Scholar
Bixler, H.J. 1996. Recent developments in manufacturing and marketing carrageenan. Hydrobiologia326/327: 35–57.10.1007/BF00047785Search in Google Scholar
Bodeau-Bellion, C. 1983. Analysis of carrageenan structure. Physiol. Vég.21: 785–793.Search in Google Scholar
Brenden, P.Ch. and K.T. Bird. 1994. Effects of environmental factors on carrageenan from Gymnogongrus griffithsiae (Gigartinales, Rhodophyta). J. Appl. Phycol.6: 371–380.Search in Google Scholar
Cheney, D.P., A.H. Luistro and P.M. Bradley. 1987. Carrageenan analysis of tissue cultures and whole plants of Agardhiella subulata. Hydrobiologia151/152: 161–166.10.1007/BF00046122Search in Google Scholar
Chopin, T., M.D. Hanisak, F.E. Koehn, J. Mollion and S. Moreau. 1990. Studies on carrageenan and effects of seawater phosphorus concentration on carrageenan content and growth of Agardhiella subulata (C. Agardh) Kraft and Wynne (Rhodophyceae, Solieriaceae). J. Appl. Phycol.2: 3–16.Search in Google Scholar
Correa-Díaz, F., R. Aguilar-Rosas and L.E. Aguilar-Rosas. 1990. Infrared analysis of eleven carrageenophytes from Baja California, Mexico. Hydrobiologia204/205: 609–614.10.1007/978-94-009-2049-1_89Search in Google Scholar
Craigie, J.S. 1990. Cell walls. In: (K.M. Cole and R.G. Sheath, eds) Biology of the red algae. Cambridge University Press, Cambridge. pp. 221–258.Search in Google Scholar
Craigie, J.S. and C. Leigh. 1978. Carrageenan and agar. In: (J.A. Hellebust and J.A. Craigie, eds) Handbook of phycological methods: physiological and biochemical methods. Cambridge University Press, Cambridge. pp. 109–131.Search in Google Scholar
Dawes, C.J., N.F. Stanley and D.J. Stancioff. 1977. Seasonal and reproductive aspects of plant chemistry and iota carrageenan from Floridian Eucheuma (Rhodophyta, Gigartinales). Bot. Mar.20: 137–147.Search in Google Scholar
Deslandes, E., J.Y. Floc'h, C. Bodeau-Bellion, D. Brault and J.P. Braud. 1985. Evidence for ι-carrageenans in Solieria chordalis (Solieriaceae) and Calliblepharis jubata, Calliblepharis ciliate, Cystoclonium purpureum (Rhodophyllidaceae). Bot. Mar.28: 317–318.Search in Google Scholar
Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Roberts and F. Smith. 1956. Colorimetric method for determination of sugar and related substances. Anal. Chem.28: 350–356.10.1021/ac60111a017Search in Google Scholar
Falshaw, R., H.J. Bixler and K. Johndro. 2001. Structure performance of commercial kappa-2 carrageenan. I. Structure analysis. Food Hydrocolloids15: 441–452.10.1016/S0268-005X(01)00066-2Search in Google Scholar
Fostier, A.H., J.M. Kornprobst and G. Combaut. 1992. Chemical composition and rheological properties of carrageenans from two Senegalese Solieriaceae Anatheca montagnei Schmitz and Meristotheca senegalensis Feldmann. Bot. Mar.35: 351–355.10.1515/botm.1992.35.4.351Search in Google Scholar
Freile-Pelegrín, Y. and D. Robledo. 1997. Effects of season on the agar content and chemical characteristics of Gracilaria cornea from Yucatán, Mexico. Bot. Mar.40: 285–290.10.1515/botm.1997.40.1-6.285Search in Google Scholar
Greer, C.W. and W. Yaphe. 1984. Enzymatic analysis of carrageenans: structure of carrageenan from Eucheuma nudum. Hydrobiologia116/117: 563–567.10.1007/BF00027747Search in Google Scholar
Hoffmann, R.A., D. Gidley, D. Cooke and W.J. Frith. 1995. Effect of isolation procedures on the molecular composition and physical properties of Eucheuma cottonii carrageenan. Food Hydrocolloids9: 281–289.10.1016/S0268-005X(09)80259-2Search in Google Scholar
Jackson, S.G. and E.L. McCandless. 1978. Simple, rapid, turbidimetric determination of inorganic sulfate and/or protein. Analyt. Bio.90: 802–808.Search in Google Scholar
Lawson, C.J., D.A. Rees, D.J. Stancioff and N.F. Stanley. 1973. Carrageenans. Part VIII. Repeating structures of galactan sulfates from Furcellaria fastigiata, Gigartina canaliculata, Gigartina chamissoi, Gigartina atropurpurea, Ahnfeltia durvillaei, Gymnogongrus furcellatus, Eucheuma cottonii, Eucheuma spinosum, Eucheuma isiforme, Eucheuma uncinatum, Aghardhiella tenera, Pachymenia hymantophora, and Gloiopeltis cervicornis. J. Chem. Soc. Perkin I: 2177–2182.Search in Google Scholar
Matsuhiro, B. and A. Zanlungo. 1983. Colorimetric determination of 3,6-anhydrogalactose in polysaccharides from red seaweeds. Carbohydr. Res.118: 276–279.Search in Google Scholar
McCandless, E., W.T. Okada, J.N.A. Lott, C.M. Vollmer and E.M. Gordon-Mills. 1977. Structural studies of Chondrus crispus: the effect of extraction of carrageenan. Can. J. Bot.55: 2053–2064.10.1139/b77-231Search in Google Scholar
McHugh, D.J. 2001. Prospects for seaweed production in developing countries. FAO Fisheries Circular 986. Rome, Italy. pp 28.Search in Google Scholar
Muñoz, J., Y. Freile-Pelegrín and D. Robledo. 2004. Mariculture of Kappaphycus alvarezii (Rhodophyta, Solieriaceae) color strains in tropical waters of Yucatán, Mexico. Aquaculture239: 161–177.10.1016/j.aquaculture.2004.05.043Search in Google Scholar
Pereira, L. and J.F. Mesquita. 2003. Carrageenophytes of occidental Portuguese coast: 1-spectroscopic analysis in eight carrageenophytes from Buarcos bay. Biomol. Eng.20: 223–228.10.1016/S1389-0344(03)00056-XSearch in Google Scholar
Pérez-Enríquez, R. 1996. Growth of Eucheuma isiforme (C. Agardh) J. Agardh on experimental rafts off the coast of Yucatán state, Mexico. J. Appl. Phycol.8: 27–28.Search in Google Scholar
Piculell, L. 1995. Gelling carrageenans. In: (A.M. Stephen, ed.) Food polysaccharides and their applications. Marcel Dekker Inc., New York. pp. 205–244.Search in Google Scholar
Robledo, D. 2005. The seaweed resources of México. In: (A.T. Critchley and M. Ohno, eds) Seaweed resources of the world. JICA, Japan. pp. 331–342.Search in Google Scholar
Rochas, C., M. Lahaye and W. Yaphe. 1986. Sulfate content of carrageenan and agar determined by infrared spectroscopy. Bot. Mar.29: 335–340.10.1515/botm.1986.29.4.335Search in Google Scholar
Saito, R. and E.C. de Oliveira. 1990. Chemical screening of Brazilian marine algae producing carrageenans. Hydrobiologia204/205: 585–588.10.1007/BF00040291Search in Google Scholar
Santos, G.A. 1989. Carrageenans of species of Eucheuma J. Agardh and Kappaphycus Doty (Solieriaceae, Rhodophyta). Aquat. Bot.36: 55–67.10.1016/0304-3770(89)90091-0Search in Google Scholar
Stancioff, D.J. and N.F. Stanley. 1969. Infrared and chemical studies on algal polysaccharides. Proc. Int. Seaweed Symp.6: 595–609.Search in Google Scholar
Stanley, N. 1987. Production, properties and uses of carrageenan. In: (D.J. McHugh, ed.) Production and utilization of products from commercial seaweeds. FAO Fisheries Technical Paper 288. pp. 116–146.Search in Google Scholar
Yakovleva, I.M., I.M. Yermak, E.A. Titlyanov, A.O. Baranova and V.P. Glazunov. 2001. Changes in growth rate, anatomy and polysaccharide content of a sterile form of Tichocarpus crinitus (Gmel.) Rupr. (Rhodophyta, Tichocarpaceace) grown under different photon irradiances in the sea of Japan, Russia. Bot. Mar.44: 493–500.Search in Google Scholar
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Articles in the same Issue
- Morphological and molecular evidence for multiple invasions of Codium fragile in Atlantic Canada
- Response of microphytobenthos to flow and trophic variation in two South African temporarily open/closed estuaries
- The prostrate system of the Gelidiales: diagnostic and taxonomic importance
- Variations in lipid composition among different developmental stages of Gracilaria verrucosa (Rhodophyta)
- Intra-thallus phlorotannin content and antioxidant activity in Phaeophyceae of temperate waters
- Volatile compounds from some Black Sea red algae and their chemotaxonomic application
- Tissue age as a factor affecting carrageenan quantity and quality in farmed Kappaphycus striatum (Schmitz) Doty ex Silva
- Carrageenan of Eucheuma isiforme (Solieriaceae, Rhodophyta) from Yucatán, Mexico. I. Effect of extraction conditions
- Carrageenan of Eucheuma isiforme (Solieriaceae, Rhodophyta) from Yucatán, Mexico. II. Seasonal variations in carrageenan and biochemical characteristics
- Evaluation of the structures of galactans from Carpopeltis sp., a member of the Halymeniales in the western Indian Ocean
- Evaluation of beneficial bacteria from mangrove soil
- Marine fungi on Nypa fruticans in Thailand