Properties of the docosahexaenoic acid-producer Schizochytrium mangrovei Sk-02: effects of glucose, temperature and salinity and their interaction
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Panida Unagul
Abstract
A number of variables potentially affecting docosahexaenoic acid (DHA) formation in Schizochytrium mangrovei Sk-02 were studied in shaken flasks using yeast extract (10 g l-1) as a nitrogen source. Usage of fructose, galactose, glucose, glycerol and mannose (60 g l-1) in yeast extract-containing media resulted in production of 3–4 g l-1 DHA, with a lower value for galactose. The highest DHA-content (28% w/w) was observed with glycerol. Disaccharides (maltose and lactose) resulted in poor growth. At a fixed glucose level (60 g l-1), biomass and DHA-levels reached a maximum at 25 g l-1 artificial sea salts (ASS). Replacement of ASS by various sodium salts reduced biomass and DHA-yields. Initial medium pH (4–7) had little effect on biomass or DHA-formation, but increasing temperature (25–40°C) negatively affected biomass and DHA-content, though not the ratio of saturated to unsaturated fatty acids. In addition, various combinations of glucose (45, 60 and 75 g-1), temperature (25 and 30°C) and salinity (5, 15 and 25 g l-1 ASS) were tested. In general, both biomass and DHA-levels (as g l-1) increased with increasing glucose, whereas DHA-, but not biomass levels were negatively affected by temperature. The DHA-weight content decreased with temperature. The effect of salinity was not clearly defined. Maximal DHA-level obtained was 4.7 g l-1 (75 g l-1 glucose, 15–25 g l-1 ASS and 25°C). We calculate that in a standard medium, only 42% of glucose consumed was directly converted into fatty acids.
References
Ashford, A., W.R. Barclay, C.A. Weaver, T.H. Giddings and S. Zeller. 2000. Electron microscopy may reveal structures of docosahxaenoic acid rich oil within Schizochytrium sp. Lipids35: 1377–1386.10.1007/s11745-000-0655-2Search in Google Scholar
Bahnweg, G. 1979a. Studies on the physiology of Thraustochytriales. I. Growth requirements and nitrogen nutrition of Thraustochytrium sp., Schizochytrium sp., Ulkenia sp. and Labyrinthuloides spp. Veröff.Inst. Meeresforsch. Bremerh.17: 245–258.Search in Google Scholar
Bahnweg, G. 1979b. Studies on the physiology of Thraustochytriales. II. Carbon nutrition of Thraustochytrium sp., Schizochytrium sp., Ulkenia sp. and Labyrinthuloides spp. Veröff.Inst. Meeresforsch. Bremerh.17: 269–273.Search in Google Scholar
Barclay, W.R. 1994. Process for growing Thraustochytrium and Schizochytrium using non-chloride salts to produce a micro-floral biomass having omega-3 highly unsaturated fatty acids. US patent no. 5, 340, 742.Search in Google Scholar
Bowles, R.D. 1997. Production of n-3 polyunsaturated fatty acids by thraustochytrids, physiology and optimization. PhD. Thesis, School of Biological Sciences, Portsmouth University, UK. pp. 151–160.Search in Google Scholar
Bowles, R.D., A.E. Hunt, G.B. Bremer, M.G. Duchars and R.D. Eaton. 1999. Long-chain n-3 polyunsaturated fatty acid production by members of the marine protistan group the thraustochytrids: screening of isolates and optimization of docosahexaenoic acid production. J. Biotechnol.70: 193–202.10.1016/S0079-6352(99)80112-XSearch in Google Scholar
Cossins, A.R., P.A. Murray, A.Y. Gracey, J. Logue, S. Polley, M. Caddick, S. Brooks, T. Postle and N. Maclean. 2002. The role of desaturases in cold-induced lipid desaturation. Biochem. Soc. Trans.30: 1082–1086.10.1042/bst0301082Search in Google Scholar
Fan, K.W., F. Chen, L.L.P. Vrijmoed and E.B.G. Jones. 2000. Utilization of food processing waste by thraustochytrids. Fungal Divers.5: 185–194.Search in Google Scholar
Fan, K.W., L.L.P. Vrijmoed and E.B.G. Jones. 2002. Physiological studies of subtropical mangrove thraustochytrids. Bot. Mar.45: 50–57.10.1515/BOT.2002.006Search in Google Scholar
Fofonoff, N.P. and H. Bryden. 1975. Specific gravity and density of seawater at atmospheric pressure. J. Mar. Res.33: 69–82.Search in Google Scholar
Kumon, Y., R. Yokoyama, T. Yokochi, D. Honda and T. Nakahara. 2003. A new labyrinthulid isolate, which solely produces n-6 docospentanoic acid. Appl. Microbiol. Biotechnol.63: 22–28.Search in Google Scholar
Leano, E.M., R.S.J. Gapasin, B. Polohan and L.L.P. Vrijmoed. 2003. Growth and fatty acid production of thraustochytrids from Panay mangroves, Philippines. Fungal Divers.12: 111–122.Search in Google Scholar
Metz J.G., P. Roessler, D. Facciotti, C. Levering, F. Dittrich, M. Lassner, R. Valentine, K. Lardizabal, F. Domergue, A. Yamada, K. Yazawa, V. Knauf and J. Browse. 2001. Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science293: 290–293.10.1126/science.1059593Search in Google Scholar
Montine, T.J. and J.D. Morrow. 2005. Fatty acid oxidation in the pathogenesis of Alzheimer's disease. Am. J. Pathol.166: 1283–1289.10.1016/S0002-9440(10)62347-4Search in Google Scholar
Oren, A. 1992. Bioenergetic aspects of halophilism. Microbiol. Molec. Biol. Rev.63: 334–348.10.1128/MMBR.63.2.334-348.1999Search in Google Scholar PubMed PubMed Central
Ratlegde, C. 2002. Regulation of lipid accumulation in oleaginous micro-organisms. Biochem. Soc. Trans.30: 1047–1050.10.1042/bst0301047Search in Google Scholar PubMed
Ratledge, C. 2004. Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie86: 817–824.10.1016/j.biochi.2004.09.017Search in Google Scholar PubMed
Sijtsma, L. and M.E. de Swaaf. 2004. Biotechnological production and applications of the ω-3 polyunsaturated fatty acid docosahexaenoic acid. Appl. Microbiol. Biotechnol.64: 146–153.10.1007/s00253-003-1525-ySearch in Google Scholar PubMed
Ward, O.P. and A. Singh. 2005. Omega-3/6 fatty acids: alternative sources of production. Proc. Biochem. 40: 3627–13652.10.1016/j.procbio.2005.02.020Search in Google Scholar
Wu, S.-T. and L-P. Lin. 2003. Application of response surface methodology to optimize docosahexaenoic acid production by Schizochytrium sp. S31. Food Biochem.27: 127–139.10.1111/j.1745-4514.2003.tb00271.xSearch in Google Scholar
Yaguchi, T., S. Tanaka, T. Yokochi, T. Nakahara and T. Higashira. 1997. Production of high yields of docosahexaenoic acid by Schizochytrium sp. strain SR21. JAOCS74: 1431–1434.10.1007/s11746-997-0249-zSearch in Google Scholar
Yokochi T., D. Honda, T. Higashihara and T. Nakahara. 1998. Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21. Appl. Microbiol. Biotechnol.49: 72–76.10.1007/s002530051139Search in Google Scholar
©2005 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Author index volume 48 (2005)
- Contents volume 48 (2005)
- Genus/Species index volume 48 (2005)
- Special Issues of Botanica Marina
- 5th Asia Mycological Congress and 9th International Marine and Freshwater Mycology Symposium, Chiang Mai, Thailand, 14–19 November 2004
- Molecular approaches for assessing fungal diversity in marine substrata
- Diversity of marine fungi from Egyptian Red Sea mangroves
- Marine fungi from the Bahamas Islands
- Marine fungi on Nypa fruticans in Thailand
- Abundance of thraustochytrids on fallen decaying leaves of Kandelia candel and mangrove sediments in Futian National Nature Reserve, China
- Screening of marine fungi for lignocellulose-degrading enzyme activities
- Properties of the docosahexaenoic acid-producer Schizochytrium mangrovei Sk-02: effects of glucose, temperature and salinity and their interaction
- A systematic reassessment of the marine ascomycetes Torpedospora and Swampomyces
- Long-term acclimation to UV radiation: effects on growth, photosynthesis and carbonic anhydrase activity in marine diatoms
- Histioneis (Dinophysiales, Dinophyceae) from the western Pacific Ocean
- Molecular investigation reveals epi/endophytic extrageneric kelp (Laminariales, Phaeophyceae) gametophytes colonizing Lessoniopsis littoralis thalli
- Total dietary fiber content in Hawaiian marine algae
- The antibacterial compound sulphoglycerolipid 1-0 palmitoyl-3-0(6′-sulpho-α-quinovopyranosyl)-glycerol from Sargassum wightii Greville (Phaeophyceae)
- Evidence for vertical growth in Zostera noltii Hornem.
- Acknowledgement volume 48 (2005)
Articles in the same Issue
- Author index volume 48 (2005)
- Contents volume 48 (2005)
- Genus/Species index volume 48 (2005)
- Special Issues of Botanica Marina
- 5th Asia Mycological Congress and 9th International Marine and Freshwater Mycology Symposium, Chiang Mai, Thailand, 14–19 November 2004
- Molecular approaches for assessing fungal diversity in marine substrata
- Diversity of marine fungi from Egyptian Red Sea mangroves
- Marine fungi from the Bahamas Islands
- Marine fungi on Nypa fruticans in Thailand
- Abundance of thraustochytrids on fallen decaying leaves of Kandelia candel and mangrove sediments in Futian National Nature Reserve, China
- Screening of marine fungi for lignocellulose-degrading enzyme activities
- Properties of the docosahexaenoic acid-producer Schizochytrium mangrovei Sk-02: effects of glucose, temperature and salinity and their interaction
- A systematic reassessment of the marine ascomycetes Torpedospora and Swampomyces
- Long-term acclimation to UV radiation: effects on growth, photosynthesis and carbonic anhydrase activity in marine diatoms
- Histioneis (Dinophysiales, Dinophyceae) from the western Pacific Ocean
- Molecular investigation reveals epi/endophytic extrageneric kelp (Laminariales, Phaeophyceae) gametophytes colonizing Lessoniopsis littoralis thalli
- Total dietary fiber content in Hawaiian marine algae
- The antibacterial compound sulphoglycerolipid 1-0 palmitoyl-3-0(6′-sulpho-α-quinovopyranosyl)-glycerol from Sargassum wightii Greville (Phaeophyceae)
- Evidence for vertical growth in Zostera noltii Hornem.
- Acknowledgement volume 48 (2005)
