Startseite Lebenswissenschaften Screening of marine fungi for lignocellulose-degrading enzyme activities
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

Screening of marine fungi for lignocellulose-degrading enzyme activities

  • Wen Luo , Lilian L.P. Vrijmoed und E.B. Gareth Jones
Veröffentlicht/Copyright: 8. Dezember 2005
Botanica Marina
Aus der Zeitschrift Band 48 Heft 5

Abstract

Twenty-nine fungal isolates collected from tropical and subtropical mangrove/marine habitats were screened for the presence of lignocellulose-degrading enzyme activities in agar media. These fungi were ascomycetes, except for a basidiomycete, Calathella mangrovei, and a mitosporic fungus, Cirrenalia tropicalis. Endoglucanase and xylanase were the most common enzymes produced. However, none of the fungi exhibited an ability to decolourise Poly-R-478 dye, indicating the lack of ligninolytic peroxidases. Three groups of fungi were categorised according to their cellulolytic, xylanolytic, and ligninolytic enzymes. Group I contained 21 isolates (ca. 72% of the test fungi) able to produce the three enzymes: endoglucanase, xylanase and laccase. Group II comprised 2 isolates lacking the ability to utilise filter paper and/or xylan, whereas Group III consisted of 6 isolates (ca. 21%) with no laccase activity. Five laccase-producing isolates selected for growth on artificial seawater (ASW) agar supplemented with 2,2′-azino-bis-3-ethylbenz-thiazoline-6-sulfonic acid (ABTS), a substrate for this enzyme, exhibited no laccase activity. Further testing in ASW liquid medium with Hypoxylon species B and Halorosellinia oceanica showed that laccase activity was detectable from culture supernatants that had been dialysed against distilled water. Therefore, care is required in assessing laccase activity when using an agar plate assay in the presence of ASW.

Keywords: endoglucanase; laccase; marine fungi; seawater inhibition; xylanase
:
Corresponding author

References

Biely, P., M. Vranska and M. Claeyssens. 1991. The endo-1,4-β-glucanase I from Trichoderma reesei: action on β-1,4-oligomers and polymers derived from D-glucose and D-xylose. Euro. J. Biochem.200: 157–163.Suche in Google Scholar

Boominathan, K. and C.A. Reddy. 1992. Fungal degradation of lignin: biotechnological applications. In: (D.K. Arora, R.P. Elander and K.G. Mukerji, eds) Handbook of applied mycology. Vol. 4: Fungal biotechnology. Marcel Dekker, New York. pp. 763–822.Suche in Google Scholar

Bourbonnais, R. and M.G. Paice. 1990. Oxidation of non-phenolic substrates – an expanded role for laccase in lignin biodegradation. FEBS Lett.267: 99–102.10.1016/0014-5793(90)80298-WSuche in Google Scholar

Chung, N., I.-S. Lee, H.-S. Song and W.-G. Bang. 2000. Mechanisms used by white-rot fungi to degrade lignin and toxic chemicals. J. Microbiol. Biotechnol.10: 737–752.Suche in Google Scholar

Corbett, N.H. 1965. Micro-morphological studies on the degradation of lignified cell walls by Ascomycetes and Fungi Imperfecti. J. Inst. Wood Sci.4: 18–29.Suche in Google Scholar

Eriksson, K.-E., R.A. Blanchette and P. Ander. 1990. Microbial and enzymatic degradation of wood and wood components. Springer-Verlag, Berlin. pp. 407.Suche in Google Scholar

Eslyn, W.E., T.K. Kirk and M.J. Effland. 1975. Changes in the chemical composition of wood caused by six soft-rot fungi. Phytopathology65: 473–476.10.1094/Phyto-65-473Suche in Google Scholar

Eyzaguire, J., J. Scarpa, A. Belancic and J. Steiner. 1992. The xylanase system of Penicilium purpurogenum. In: (J. Visser, G. Beldman, M.A. Kuster-van Someren and A.G.J. Voragen, eds) Xylans and xylanases. Elsevier, Amsterdam. pp. 505–511.Suche in Google Scholar

Fenn, P. and T.K. Kirk. 1981. Relationship of nitrogen to the onset and suppression of ligninolytic activity and secondary metabolism in Phanerochaete chrysosporium.Arch. Microbiol.130: 59–65.10.1007/BF00527073Suche in Google Scholar

Filho, E.X.F., J. Puls and M.P. Coughlan. 1993. Physicochemical and catalytic properties of a low-molecular-weight endo-1,4,-b-xylanase from Myrothecium verrucaria. Enzy. Microb. Technol.15: 535–540.Suche in Google Scholar

Garzillo, A.M.V., M.C. Colao, C. Caruso, C. Caporale, D. Cellette and V. Buonocore. 1998. Laccase from the white-rot fungus Trametes trogii. Appl. Microbiol. Biotechnol.49: 545–551.10.1007/s002530051211Suche in Google Scholar

Gianfreda, L., F. Xu and J.-M. Bollag. 1999. Laccases: a useful group of oxidoreductive enzymes. Bioremed. J.3: 1–25.10.1080/10889869991219163Suche in Google Scholar

Gold, M.H., J.K. Glenn and M. Alic. 1988. Use of polymeric dyes in lignin biodegradation assays. Methods Enzymol.161: 74–78.10.1016/0076-6879(88)61011-1Suche in Google Scholar

Haider, K. and J. Trojanowski. 1975. Decomposition of specifically 14C-labelled phenols and dehydropolymers of coniferyl alcohol as models for lignin degradation by soft and white rot fungi. Arch. Microbiol.105: 33–41.10.1007/BF00447109Suche in Google Scholar

Hatakka, A. 1994. Lignin-modifying enzymes from selected white-rot fungi: production and role in lignin degradation. FEMS Microbiol. Rev.13: 125–135.10.1111/j.1574-6976.1994.tb00039.xSuche in Google Scholar

Hyde, K.D., V.V. Sarma and E.B.G. Jones. 2000. Morphology and taxonomy of higher marine fungi. In: (K.D. Hyde and S.B. Pointing, eds) Marine mycology: a practical approach. Fungal diversity research series 1. Fungal Diversity Press, Hong Kong. pp. 171–204.Suche in Google Scholar

Jones, E.B.G. 1993. Tropical marine fungi. In: (S. Issac, J.C. Fankland, R. Watling and A.J.S. Whalley, eds) Aspects of tropical mycology. Cambridge University Press, Cambridge. pp. 73–90.Suche in Google Scholar

Kerr, A.J. and D.A.I. Goring. 1975. The ultrastructural arrangement of the wood cell wall. Cellul. Chem. Technol.9: 563–573.Suche in Google Scholar

Kersten, P.J., B. Kalyanaraman, K.E. Hammel, B. Reinhammer and T.K. Kirk. 1990. Comparison of lignin peroxidase, horseradish peroxidase and laccase in the oxidation of methoxybenzenes. Biochem. J.268: 475–480.Suche in Google Scholar

Keyser, P., T.K. Kirk and J.G. Zeikus. 1978. Ligninolytic enzyme system of Phanerochaete chrysosporium: synthesized in the absence of lignin in response to nitrogen starvation. J. Bacteriol.135: 790–797.10.1128/jb.135.3.790-797.1978Suche in Google Scholar

Koudelka, G.B. and M.J. Ettinger. 1988. Fluoride effects on the activity of Rhus laccase and the catalytic mechanism under steady-state conditions. J. Biol. Chem.263: 3698–3705.10.1016/S0021-9258(18)68981-1Suche in Google Scholar

Kuwahara, M., J.K. Glenn, M.A. Morgan and M.H. Gold. 1984. Separation and characterization of two extracellular H2O2-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Lett.169: 247–250.10.1016/0014-5793(84)80327-0Suche in Google Scholar

Lappalainen, A. 1986. Purification and characterization of xylanolytic enzymes from Trichoderma reesei. Biotechnol. Appl. Biochem.8: 437–448.Suche in Google Scholar

Leightley, L.E. 1980. Wood decay activities of marine fungi. Bot. Mar.23: 387–395.Suche in Google Scholar

Li, K., F. Xu and K.-E. Eriksson. 1999. Comparison of fungal laccases and redox mediators in oxidation of a nonphenolic lignin model compound. Appl. Env. Microbiol.65: 2654–2660.10.1128/AEM.65.6.2654-2660.1999Suche in Google Scholar

Lyman, J. and R.H. Fleming. 1940. Composition of sea water. J. Mar. Res.3: 134–136.Suche in Google Scholar

Mayer, A.M. and R.C. Staples. 2002. Laccase: new functions for an old enzyme. Phytochemistry60: 551–565.10.1016/S0031-9422(02)00171-1Suche in Google Scholar

Mishra, C., S. Keskar and M. Rao. 1984. Production and properties of extracellular endoxylanase from Neurospora crassa. Appl. Env. Microbiol.48: 224–228.10.1128/aem.48.1.224-228.1984Suche in Google Scholar

Mouzouras, R. 1989. Decay of mangrove wood by marine fungi. Bot. Mar.32: 65–69.10.1515/botm.1989.32.1.65Suche in Google Scholar

Niku-Paavola, M.L., L. Raaska and M. Itavaara. 1990. Detection of white rot fungi by a non-toxic stain. Mycol. Res.94: 27–31.10.1016/S0953-7562(09)81260-4Suche in Google Scholar

Nilsson, T., G. Daniel, T.K. Kirk and J.R. Obst. 1989. Chemistry and microscopy of wood decay by some higher Asco-mycetes. Holzforschung43: 11–18.10.1515/hfsg.1989.43.1.11Suche in Google Scholar

Ollikka, P., K. Alhonmaki, V. Leppanen, T. Glumoff, T. Raijola and V.M. Suominen. 1993. Decolourisation of azo, triphenyl methane, heterocyclic, and polymeric dyes by lignin peroxidase isoenzymes from Phanerochaete chrysosporium. Appl. Env. Microbiol.59: 4010–4016.Suche in Google Scholar

Phillips, L.E. and T.J. Leonard. 1976. Benzidine as a substrate for measuring phenoloxidase activity in crude cell-free extracts of Schizophyllum commune. Mycologia68: 277–285.10.1080/00275514.1976.12019910Suche in Google Scholar

Pointing, S.B., L.L.P. Vrijmoed and E.B.G. Jones. 1998. A qualitative assessment of lignocellulose degrading enzyme activity in marine fungi. Bot. Mar.41: 293–298.10.1515/botm.1998.41.1-6.293Suche in Google Scholar

Raghukumar, C., S. Raghukumar, A. Chinnaraj, D. Chandramohan, T.M. D'Souza and C.A. Reddy. 1994. Laccase and other lignocellulose modifying enzymes of marine fungi isolated from the coast of India. Bot. Mar.37: 515–523.10.1515/botm.1994.37.6.515Suche in Google Scholar

Rohrmann, S. and H.P. Molitoris. 1992. Screening of wood-degrading enzymes in marine fungi. Can. J. Bot.70: 2116–2123.10.1139/b92-263Suche in Google Scholar

Sutherland, J.B., D.L. Crawford and M.K. Speedie. 1982. Decomposition of 14C-labeled maple and spruce lignin by marine fungi. Mycologia74: 511–513.10.1080/00275514.1982.12021539Suche in Google Scholar

Teather, R.M. and P.J. Wood. 1982. Use of Congo-red-polysaccharide interactions in enumeration and characterisation of cellulolytic bacteria from the bovine rumen. Appl. Env. Microbiol.43: 777–780.10.1128/aem.43.4.777-780.1982Suche in Google Scholar

Tien, M. and T.K. Kirk. 1988. Lignin peroxidase of Phanerochaete chrysosporium. Methods Enzymol.161: 238–249.10.1016/0076-6879(88)61025-1Suche in Google Scholar

Tuor, U., K. Winterhalter and A. Fiechter. 1995. Enzymes of white-rot fungi involved in lignin degradation and ecological determinants for wood decay. J. Biotechnol.41: 1–17.10.1016/0168-1656(95)00042-OSuche in Google Scholar

Wesenberg, D., I. Kyriakides and S.N. Agathos. 2003. White-rot fungi and their enzymes for the treatment of industrial dye effluents. Biotechnol. Adv.22: 161–187.10.1016/j.biotechadv.2003.08.011Suche in Google Scholar

Whalley, A.J.S., E.B.G. Jones and S.A. Alias. 1994. The Xylariaceae (Ascomycetes) of mangroves in Malaysia and South East Asia. Nova Hedwigia59: 207–218.Suche in Google Scholar

Wood, T.M. and K.M. Bhat. 1988. Methods for measuring cellulase activities. Methods Enzymol.160: 87–126.10.1016/0076-6879(88)60109-1Suche in Google Scholar

Worrall, J.J., S.E. Anagnost and R.A. Zabel. 1997. Comparison of wood decay among diverse lignicolous fungi. Mycologia89: 199–219.10.1080/00275514.1997.12026772Suche in Google Scholar

Xu, F. 1996. Oxidation of phenols, anilines, and benzenethiols by fungal laccases: correlation between activity and redox potentials as well as halide inhibition. Biochemistry35: 7608–7614.Suche in Google Scholar

Published Online: 2005-12-08
Published in Print: 2005-12-01

©2005 by Walter de Gruyter Berlin New York

Artikel in diesem Heft

  1. Author index volume 48 (2005)
  2. Contents volume 48 (2005)
  3. Genus/Species index volume 48 (2005)
  4. Special Issues of Botanica Marina
  5. 5th Asia Mycological Congress and 9th International Marine and Freshwater Mycology Symposium, Chiang Mai, Thailand, 14–19 November 2004
  6. Molecular approaches for assessing fungal diversity in marine substrata
  7. Diversity of marine fungi from Egyptian Red Sea mangroves
  8. Marine fungi from the Bahamas Islands
  9. Marine fungi on Nypa fruticans in Thailand
  10. Abundance of thraustochytrids on fallen decaying leaves of Kandelia candel and mangrove sediments in Futian National Nature Reserve, China
  11. Screening of marine fungi for lignocellulose-degrading enzyme activities
  12. Properties of the docosahexaenoic acid-producer Schizochytrium mangrovei Sk-02: effects of glucose, temperature and salinity and their interaction
  13. A systematic reassessment of the marine ascomycetes Torpedospora and Swampomyces
  14. Long-term acclimation to UV radiation: effects on growth, photosynthesis and carbonic anhydrase activity in marine diatoms
  15. Histioneis (Dinophysiales, Dinophyceae) from the western Pacific Ocean
  16. Molecular investigation reveals epi/endophytic extrageneric kelp (Laminariales, Phaeophyceae) gametophytes colonizing Lessoniopsis littoralis thalli
  17. Total dietary fiber content in Hawaiian marine algae
  18. The antibacterial compound sulphoglycerolipid 1-0 palmitoyl-3-0(6′-sulpho-α-quinovopyranosyl)-glycerol from Sargassum wightii Greville (Phaeophyceae)
  19. Evidence for vertical growth in Zostera noltii Hornem.
  20. Acknowledgement volume 48 (2005)
https://doi.org/10.1515/BOT.2005.051
Schlagwörter für diesen Artikel
endoglucanase; laccase; marine fungi; seawater inhibition; xylanase

Artikel in diesem Heft

  1. Author index volume 48 (2005)
  2. Contents volume 48 (2005)
  3. Genus/Species index volume 48 (2005)
  4. Special Issues of Botanica Marina
  5. 5th Asia Mycological Congress and 9th International Marine and Freshwater Mycology Symposium, Chiang Mai, Thailand, 14–19 November 2004
  6. Molecular approaches for assessing fungal diversity in marine substrata
  7. Diversity of marine fungi from Egyptian Red Sea mangroves
  8. Marine fungi from the Bahamas Islands
  9. Marine fungi on Nypa fruticans in Thailand
  10. Abundance of thraustochytrids on fallen decaying leaves of Kandelia candel and mangrove sediments in Futian National Nature Reserve, China
  11. Screening of marine fungi for lignocellulose-degrading enzyme activities
  12. Properties of the docosahexaenoic acid-producer Schizochytrium mangrovei Sk-02: effects of glucose, temperature and salinity and their interaction
  13. A systematic reassessment of the marine ascomycetes Torpedospora and Swampomyces
  14. Long-term acclimation to UV radiation: effects on growth, photosynthesis and carbonic anhydrase activity in marine diatoms
  15. Histioneis (Dinophysiales, Dinophyceae) from the western Pacific Ocean
  16. Molecular investigation reveals epi/endophytic extrageneric kelp (Laminariales, Phaeophyceae) gametophytes colonizing Lessoniopsis littoralis thalli
  17. Total dietary fiber content in Hawaiian marine algae
  18. The antibacterial compound sulphoglycerolipid 1-0 palmitoyl-3-0(6′-sulpho-α-quinovopyranosyl)-glycerol from Sargassum wightii Greville (Phaeophyceae)
  19. Evidence for vertical growth in Zostera noltii Hornem.
  20. Acknowledgement volume 48 (2005)
Jetzt anmelden und 20% sparen
Institutioneller Zugang
Wie funktioniert Authentifizierung?
Haben Sie eine Idee, wie wir unsere Website verbessern können?
Bitte schreiben Sie uns.
© 2026 De Gruyter Brill
Heruntergeladen am 20.2.2026 von https://www.degruyterbrill.com/document/doi/10.1515/BOT.2005.051/html
Button zum nach oben scrollen