The application of 13C NMR spectroscopy to the red algal polysaccharides of selected New Zealand species of Lomentaria
-
Ian J. Miller
Abstract
The structural features of polysaccharides extracted from four New Zealand species of Lomentaria were examined. The polysaccharides retain the expected backbone structure of alternating 3-linked and 4-linked galactosyl units, with the 4-linked units containing some 3,6-anhydro-L-anhydrogalactose and galactose in the D- (40%) and L- (60%) configurations. Natural sulfation leads to twelve different units being found. Since none of the levels of 3-linked units matched those of 4-linked units, many units were in at least two different diads, and with such variation it is unlikely that there is a consistently repeating structure as such. An important diad combined 4-linked L-galactose-2,3-disulfate and L-galactose 2,3,6-trisulfate with 3-linked D-galactose-2,6-disulfate, a diad group equivalent to the principal diad in Champia polysaccharides with primary hydroxyl groups sulfated. A further important diad is 3-linked D-galactose-2,4-disulfate with 4-linked D-galactose-3-sulfate, a diad group also found in Hymenocladia sanguinea. Hence these results suggest that Lomentaria has an intermediate relationship with respect to Champia and Hymenocladia within the Rhodymeniales.
References
Ciancia, M., M.C. Matulewicz, P. Finch and A.S. Cerezo. 1993. Determination of the structures of cystocarpic carrageenans from Gigartina skottsbergii by methylation analysis and NMR spectroscopy. Carbohydr. Res.238: 241–248.10.1016/0008-6215(93)87016-LSuche in Google Scholar
Falshaw, R. and R.H. Furneaux. 1994. Carrageenan from the tetrasporic stage of Gigartina decipiens (Gigartinaceae, Rhodophyta). Carbohydr. Res.252: 171–182.10.1016/0008-6215(94)90014-0Suche in Google Scholar
Lahaye, M., W. Yaphe, M.T.P. Viet and C. Rochas. 1989. 13C NMR spectroscopic investigation of methylated and charged agarose oligosaccharides and polysaccharides. Carbohydr. Res.190: 249–265.10.1016/0008-6215(89)84129-1Suche in Google Scholar
Miller, I.J. 1997. The chemotaxonomic significance of the water soluble red algal polysaccharides. Recent Res. Devel. in Phytochem.1: 531–565.Suche in Google Scholar
Miller, I.J. 2001a. The structure of the polysaccharide from Hymenocladia sanguinea through 13C NMR spectroscopy. Bot. Mar.44: 245–251.10.1515/BOT.2001.032Suche in Google Scholar
Miller, I.J. 2001b. Evaluation of the structure of the polysaccharide from Myriogramme denticulata as determined by 13C NMR spectroscopy. Bot. Mar.44: 253–259.10.1515/BOT.2001.033Suche in Google Scholar
Miller, I.J. 2002. The structures of the polysaccharides from two Lophurella species as determined by 13C NMR spectroscopy. Bot. Mar.45: 373–379.10.1515/BOT.2002.038Suche in Google Scholar
Miller, I.J. 2003. The chemical structure of galactans from Sarcodia montagneana and from Sarcodia flabellataBot. Mar.46: 392–399.10.1515/BOT.2003.038Suche in Google Scholar
Miller, I.J. and J.W. Blunt. 1998. Desulfation of algal galactans. Carbohydr. Res.309: 39–43.10.1016/S0008-6215(98)00104-9Suche in Google Scholar
Miller, I.J. and J.W. Blunt. 2000a. New 13C NMR methods for determining the structure of algal polysaccharides. Part 1. The effect of substitution on the chemical shifts of simple diad galactans. Bot. Mar.43: 239–250.10.1515/BOT.2000.026Suche in Google Scholar
Miller, I.J. and J.W. Blunt. 2000b. New 13C NMR methods for determining the structure of algal polysaccharides. Part 2. Galactans consisting of mixed diads. Bot. Mar.43: 251–261.10.1515/BOT.2000.027Suche in Google Scholar
Miller, I.J. and J.W. Blunt. 2002. Mobility of sulfate ester during structural determinations of red algal galactans. Bot. Mar.45: 559–565.10.1515/BOT.2002.059Suche in Google Scholar
Miller, I.J. and J.W. Blunt. 2005. The structure of sulfated galactans from selected species of New Zealand Champia. Bot. Mar.48: 127–136.10.1515/BOT.2005.013Suche in Google Scholar
Miller, I.J., R. Falshaw and R.H. Furneaux. 1996. A polysaccharide fraction from the red seaweed Champia novae-zealandiae Rhodymeniales, Rhodophyta. Hydrobiologia326/327: 505–509.10.1007/978-94-009-1659-3_73Suche in Google Scholar
Rochas, C., M. Lahaye and W. Yaphe. 1986. Sulfate content of carrageenan and agar determined by infrared spectroscopy. Bot. Mar.24: 335–340.10.1515/botm.1986.29.4.335Suche in Google Scholar
Saunders, G.W. and G.T. Kraft. 1996. Small-subunit rRNA gene sequences from representatives of selected families of the Gigartinales and Rhodymeniales (Rhodophyta). 2. Recognition of the Halymeniales ord. nov. Can. J. Bot.74: 694–707.10.1139/b96-088Suche in Google Scholar
Usov, A.I., S.V. Yarotsky and A.S. Shashkov. 1980. Carbon-13 NMR spectroscopy of red algal galactans. Biopolymers19: 977–990.10.1002/bip.1980.360190504Suche in Google Scholar
Usov, A.I., E.G. Ivanova and A.S. Shashkov. 1983. Polysaccharides of the algae 33: isolation and 13C NMR spectral study of some new gel-forming polysaccharides from Japan Sea red seaweeds. Bot. Mar.26: 285–294.10.1515/botm.1983.26.6.285Suche in Google Scholar
Usov, A.I., M.I. Bilan and A.S. Shashkov. 1997. Structures of a sulfated xylogalactan from the calcareous red alga Corallina pilulifera P. et R. (Rhodophyta, Corallinaceae). Carbohydr. Res.303: 93–102.10.1016/S0008-6215(97)00136-5Suche in Google Scholar
© Walter de Gruyter Berlin New York
Artikel in diesem Heft
- First letters to the editor in Botanica Marina's “Forum” section
- Propagule release and recruitment in Porphyra perforata (Rhodophyta) from Baja California, Mexico
- Spatio-temporal variability of intertidal algal assemblages of the Slovenian coast (Gulf of Trieste, northern Adriatic Sea)
- Persistence of sea urchin (Heliocidaris erythrogramma) barrens on the east coast of Tasmania: inhibition of macroalgal recovery in the absence of high densities of sea urchins
- Biomass and taxonomic richness of epilithic cyanobacteria in a tropical intertidal rocky habitat
- Spatial variation in the structural parameters of Cymodocea nodosa seagrass meadows in the Canary Islands: a multiscaled approach
- The structure of sulfated galactans from selected species of New Zealand Champia
- The structure of the galactan from Aeodes nitidissima (Halymeniales, Rhodophyta)
- The structure of the polysaccharide from Grateloupia intestinalis in New Zealand
- Evaluation of the structures of polysaccharides from three taxa in the genus Hymenena and from Acrosorium decumbens (Rhodophyta, Delesseriaceae)
- The structure of polysaccharides from selected New Zealand species of Grateloupia
- The application of 13C NMR spectroscopy to the red algal polysaccharides of selected New Zealand species of Lomentaria
- Pseudovivipary, a new form of asexual reproduction in the seagrass Posidonia oceanica
- Comments on the papers by Ian J. Miller devoted to carbon-13 NMR spectroscopy of red algal galactans
- Response to “Comments on the papers by Ian J. Miller devoted to carbon-13 NMR spectroscopy of red algal galactans” by Anatolii I. Usov; Bot. Mar. 48 (2005): 178–180
- 8th International Phycological Congress 13–19 August 2005, Durban, South Africa
Artikel in diesem Heft
- First letters to the editor in Botanica Marina's “Forum” section
- Propagule release and recruitment in Porphyra perforata (Rhodophyta) from Baja California, Mexico
- Spatio-temporal variability of intertidal algal assemblages of the Slovenian coast (Gulf of Trieste, northern Adriatic Sea)
- Persistence of sea urchin (Heliocidaris erythrogramma) barrens on the east coast of Tasmania: inhibition of macroalgal recovery in the absence of high densities of sea urchins
- Biomass and taxonomic richness of epilithic cyanobacteria in a tropical intertidal rocky habitat
- Spatial variation in the structural parameters of Cymodocea nodosa seagrass meadows in the Canary Islands: a multiscaled approach
- The structure of sulfated galactans from selected species of New Zealand Champia
- The structure of the galactan from Aeodes nitidissima (Halymeniales, Rhodophyta)
- The structure of the polysaccharide from Grateloupia intestinalis in New Zealand
- Evaluation of the structures of polysaccharides from three taxa in the genus Hymenena and from Acrosorium decumbens (Rhodophyta, Delesseriaceae)
- The structure of polysaccharides from selected New Zealand species of Grateloupia
- The application of 13C NMR spectroscopy to the red algal polysaccharides of selected New Zealand species of Lomentaria
- Pseudovivipary, a new form of asexual reproduction in the seagrass Posidonia oceanica
- Comments on the papers by Ian J. Miller devoted to carbon-13 NMR spectroscopy of red algal galactans
- Response to “Comments on the papers by Ian J. Miller devoted to carbon-13 NMR spectroscopy of red algal galactans” by Anatolii I. Usov; Bot. Mar. 48 (2005): 178–180
- 8th International Phycological Congress 13–19 August 2005, Durban, South Africa
