Intermolecular interaction between a branching ribozyme and associated homing endonuclease mRNA
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Abstract
RNA tertiary interactions involving docking of GNRA (N; any base; R; purine) hairpin loops into helical stem structures on other regions of the same RNA are one of the most common RNA tertiary interactions. In this study, we investigated a tertiary association between a GAAA hairpin tetraloop in a small branching ribozyme (DiGIR1) and a receptor motif (HEG P1 motif) present in a hairpin structure on a separate mRNA molecule. DiGIR1 generates a 2′, 5′ lariat cap at the 5′ end of its downstream homing endonuclease mRNA by catalysing a self-cleavage branching reaction at an internal processing site. Upon release, the 5′ end of the mRNA forms a distinct hairpin structure termed HEG P1. Our biochemical data, in concert with molecular 3D modelling, provide experimental support for an intermolecular tetraloop receptor interaction between the L9 GAAA in DiGIR1 and a GNRA tetraloop receptor-like motif (UCUAAG-CAAGA) found within the HEG P1. The biological role of this interaction appears to be linked to the homing endonuclease expression by promoting post-cleavage release of the lariat capped mRNA. These findings add to our understanding of how protein-coding genes embedded in nuclear ribosomal DNA are expressed in eukaryotes and controlled by ribozymes.
©2011 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Publisher’s Note
- Publisher’s Note
- GENES AND NUCLEIC ACIDS
- Intermolecular interaction between a branching ribozyme and associated homing endonuclease mRNA
- Type V collagen-induced upregulation of capn2 (large subunit of m-calpain) gene expression and DNA fragmentation in 8701-BC breast cancer cells
- PROTEIN STRUCTURE AND FUNCTION
- High catalytic efficiency and resistance to denaturing in bacterial Rho GTPase-activating proteins
- MEMBRANES, LIPIDS, GLYCOBIOLOGY
- Cloning and functional analysis of the dpm2 and dpm3 genes from Trichoderma reesei expressed in a Saccharomyces cerevisiae dpm1Δ mutant strain
- MOLECULAR MEDICINE
- Increased expression of the multidrug resistance-associated protein 1 (MRP1) in kidney glomeruli of streptozotocin-induced diabetic rats
- CELL BIOLOGY AND SIGNALING
- Investigating the effects of physiological bile acids on GLP-1 secretion and glucose tolerance in normal and GLP-1R-/- mice
- PROTEOLYSIS
- Angiotensin I-converting enzyme (ACE) activity and expression in rat central nervous system after sleep deprivation
- Cathepsins S, B and L with aminopeptidases display β-secretase activity associated with the pathogenesis of Alzheimer’s disease
- The role of cathepsin E in terminal differentiation of keratinocytes
- Poliovirus 3C proteinase inhibition by organotelluranes
Articles in the same Issue
- Publisher’s Note
- Publisher’s Note
- GENES AND NUCLEIC ACIDS
- Intermolecular interaction between a branching ribozyme and associated homing endonuclease mRNA
- Type V collagen-induced upregulation of capn2 (large subunit of m-calpain) gene expression and DNA fragmentation in 8701-BC breast cancer cells
- PROTEIN STRUCTURE AND FUNCTION
- High catalytic efficiency and resistance to denaturing in bacterial Rho GTPase-activating proteins
- MEMBRANES, LIPIDS, GLYCOBIOLOGY
- Cloning and functional analysis of the dpm2 and dpm3 genes from Trichoderma reesei expressed in a Saccharomyces cerevisiae dpm1Δ mutant strain
- MOLECULAR MEDICINE
- Increased expression of the multidrug resistance-associated protein 1 (MRP1) in kidney glomeruli of streptozotocin-induced diabetic rats
- CELL BIOLOGY AND SIGNALING
- Investigating the effects of physiological bile acids on GLP-1 secretion and glucose tolerance in normal and GLP-1R-/- mice
- PROTEOLYSIS
- Angiotensin I-converting enzyme (ACE) activity and expression in rat central nervous system after sleep deprivation
- Cathepsins S, B and L with aminopeptidases display β-secretase activity associated with the pathogenesis of Alzheimer’s disease
- The role of cathepsin E in terminal differentiation of keratinocytes
- Poliovirus 3C proteinase inhibition by organotelluranes
