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Type V collagen-induced upregulation of capn2 (large subunit of m-calpain) gene expression and DNA fragmentation in 8701-BC breast cancer cells
-
Claudio Luparello
and
Rosalia Sirchia
Published/Copyright:
June 18, 2011
Abstract
Type V collagen is known to be over-deposited in the stroma of ductal infiltrating carcinomas of the breast. When used as a substrate, type V collagen restrains growth and invasion, and affects gene expression of 8701-BC ductal infiltrating carcinomas cells. Here we supplement existing data by demonstrating type V collagen dependent upregulation of capn2 gene expression in 8701-BC cells through differential display-PCR and Western blot assays. Furthermore, we suggest that our data obtained by centrifugal sedimentation and electrophoresis strongly suggest a correlation between calpain overproduction and DNA fragmentation, since the incubation with calpain inhibitor partly reverts the latter.
Keywords: calpain inhibitor; centrifugal sedimentation; differential display-PCR; gene expression; voltage gradient gel electrophoresis
Received: 2011-1-3
Accepted: 2011-2-15
Published Online: 2011-06-18
Published in Print: 2011-06-01
©2011 by Walter de Gruyter Berlin New York
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Keywords for this article
calpain inhibitor;
centrifugal sedimentation;
differential display-PCR;
gene expression;
voltage gradient gel electrophoresis
Articles in the same Issue
- Publisher’s Note
- Publisher’s Note
- GENES AND NUCLEIC ACIDS
- Intermolecular interaction between a branching ribozyme and associated homing endonuclease mRNA
- Type V collagen-induced upregulation of capn2 (large subunit of m-calpain) gene expression and DNA fragmentation in 8701-BC breast cancer cells
- PROTEIN STRUCTURE AND FUNCTION
- High catalytic efficiency and resistance to denaturing in bacterial Rho GTPase-activating proteins
- MEMBRANES, LIPIDS, GLYCOBIOLOGY
- Cloning and functional analysis of the dpm2 and dpm3 genes from Trichoderma reesei expressed in a Saccharomyces cerevisiae dpm1Δ mutant strain
- MOLECULAR MEDICINE
- Increased expression of the multidrug resistance-associated protein 1 (MRP1) in kidney glomeruli of streptozotocin-induced diabetic rats
- CELL BIOLOGY AND SIGNALING
- Investigating the effects of physiological bile acids on GLP-1 secretion and glucose tolerance in normal and GLP-1R-/- mice
- PROTEOLYSIS
- Angiotensin I-converting enzyme (ACE) activity and expression in rat central nervous system after sleep deprivation
- Cathepsins S, B and L with aminopeptidases display β-secretase activity associated with the pathogenesis of Alzheimer’s disease
- The role of cathepsin E in terminal differentiation of keratinocytes
- Poliovirus 3C proteinase inhibition by organotelluranes
