Association between kallikrein-related peptidases (KLKs) and macroscopic indicators of semen analysis: their relation to sperm motility
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Nashmil Emami
Abstract
Human kallikrein-related peptidases (KLKs) are a family of proteases, the majority of which are found in seminal plasma and have been implicated in semen liquefaction. Here, we examined the clinical value of seminal KLKs in the evaluation of semen quality and differential diagnosis and etiology of abnormal liquefaction and/or viscosity. KLK1–3, 5–8, 10, 11, 13, and 14 were analyzed, using highly specific ELISA assays. Samples were categorized into four clinical groups, according to their state of liquefaction and viscosity. Data were compared between the clinical groups and in association with other parameters of sperm quality, including number of motile sperms, straight line speed, sperm concentration, volume, pH, and patient age. Seminal KLKs were found to be differentially expressed in the four clinical groups. Combination of KLK2, 3, 13, and 14 and KLK1, 2, 5, 6, 7, 8, 10, 13, and 14 showed very strong discriminatory potential for semen liquefaction and viscosity, respectively. Liquefaction state was associated with several parameters of sperm motility. Finally, KLK14 was differentially expressed in asthenospermic cases. In conclusion, the expression level of several seminal plasma KLKs correlates with liquefaction and viscosity indicators of semen quality and may aid in their differential diagnosis and etiology.
©2009 by Walter de Gruyter Berlin New York
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Articles in the same Issue
- Review
- Dimeric/oligomeric DNA methyltransferases: an unfinished story
- Minireview
- Induced human pluripotent stem cells: promises and open questions
- Genes and Nucleic Acids
- Effect of a quaternary pentamine on RNA stabilization and enzymatic methylation
- Protein Structure and Function
- A soluble form of ammonia monooxygenase in Nitrosomonas europaea
- Metzincin's canonical methionine is responsible for the structural integrity of the zinc-binding site
- A dual role of the N-terminal FQQI motif in GLUT4 trafficking
- Molecular Medicine
- Peritumoral administration of GPI-anchored TIMP-1 inhibits colon carcinoma growth in Rag-2 γ chain-deficient mice
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- The effect of endogenous preproneuropeptide Y leucine 7 to proline 7 polymorphism on growth and apoptosis in primary cultured HUVECs
- Cross talk between kinin and angiotensin II receptors in mouse abdominal aorta
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- Catalytic properties of recombinant dipeptidyl carboxypeptidase from Escherichia coli: a comparative study with angiotensin I-converting enzyme
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