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The effect of endogenous preproneuropeptide Y leucine 7 to proline 7 polymorphism on growth and apoptosis in primary cultured HUVECs

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Published/Copyright: June 27, 2009
Biological Chemistry
From the journal Volume 390 Issue 9

Abstract

Neuropeptide Y (NPY) is a universally expressed neuropeptide involved in the regulation of several physiological functions. The rather common leucine7 to proline7 (L7P) polymorphism in the signal peptide of preproNPY is a functional substitution, which changes the processing and release of NPY in cells. The mutation is associated with altered lipid levels and accelerated atherosclerosis in humans. Based on previous studies, we investigated the effect of the Pro7 allele in endothelial cells, which are known to play a role in the development of atherosclerosis. Cell proliferation and apoptosis were studied in primary cultured, genotyped human umbilical vein endothelial cells (HUVECs). Our results indicate that cells with the [p.L7]+[p.P7] genotype seem to have a tendency to be more sensitive to the growth stimulating effect of NPY and less sensitive to the effect of vascular endothelial growth factor compared to cells with the [p.L7]+[p.L7] genotype. Additionally, cells with the [p.L7]+[p.P7] genotype seem to be more sensitive to apoptosis than [p.L7]+[p.L7] cells. We speculate that the L7P substitution in preproNPY might cause a state of cellular pre-senescence, leading to endothelial dysfunction. This might be one reason for the associations of the L7P polymorphism with atherosclerosis and type II diabetes found in clinical studies.

Keywords: apoptosis; cell proliferation; cellular senescence; endothelial cells
Corresponding author
Received: 2008-9-14
Accepted: 2009-5-1
Published Online: 2009-06-27
Published in Print: 2009-09-01

©2009 by Walter de Gruyter Berlin New York

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  14. The effect of endogenous preproneuropeptide Y leucine 7 to proline 7 polymorphism on growth and apoptosis in primary cultured HUVECs
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https://doi.org/10.1515/BC.2009.097
Keywords for this article
apoptosis; cell proliferation; cellular senescence; endothelial cells

Articles in the same Issue

  1. Review
  2. Dimeric/oligomeric DNA methyltransferases: an unfinished story
  3. Minireview
  4. Induced human pluripotent stem cells: promises and open questions
  5. Genes and Nucleic Acids
  6. Effect of a quaternary pentamine on RNA stabilization and enzymatic methylation
  7. Protein Structure and Function
  8. A soluble form of ammonia monooxygenase in Nitrosomonas europaea
  9. Metzincin's canonical methionine is responsible for the structural integrity of the zinc-binding site
  10. A dual role of the N-terminal FQQI motif in GLUT4 trafficking
  11. Molecular Medicine
  12. Peritumoral administration of GPI-anchored TIMP-1 inhibits colon carcinoma growth in Rag-2 γ chain-deficient mice
  13. Cell Biology and Signaling
  14. The effect of endogenous preproneuropeptide Y leucine 7 to proline 7 polymorphism on growth and apoptosis in primary cultured HUVECs
  15. Cross talk between kinin and angiotensin II receptors in mouse abdominal aorta
  16. Advanced glycation end product accumulation in rho0 cells without a functional respiratory chain
  17. Proteolysis
  18. Association between kallikrein-related peptidases (KLKs) and macroscopic indicators of semen analysis: their relation to sperm motility
  19. Catalytic properties of recombinant dipeptidyl carboxypeptidase from Escherichia coli: a comparative study with angiotensin I-converting enzyme
  20. Pharmacological inhibitors to identify roles of cathepsin K in cell-based studies: a comparison of available tools
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