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Flavivirus NS5 associates with host-cell proteins zonula occludens-1 (ZO-1) and regulating synaptic membrane exocytosis-2 (RIMS2) via an internal PDZ binding mechanism
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Karin Ellencrona
Published/Copyright:
February 7, 2009
Abstract
Dengue virus (DENV) and tick-borne encephalitis virus (TBEV) are flaviviruses, which can cause lethal hemorrhagic fever and encephalitis, respectively. Here, we demonstrate that the TBEV-NS5 and DENV-NS5 proteins use an internal binding mechanism to target human PDZ proteins. TBEV-NS5 has high affinity to regulating synaptic membrane exocytosis-2 (RIMS2) and Scribble, whereas DENV-NS5 binds primarily to the tight junction protein zonula occludens-1 (ZO-1). Targeting of TBEV-NS5 to the plasma membrane is stabilised by ZO-1; however, DENV-NS5 co-localises with ZO-1 in the nucleus. These interactions have potential important roles in the ability of flaviviruses to manipulate cell proliferation, junction permeability and the interferon pathways.
Keywords: Dengue virus (DENV); flavivirus; PDZ domains; RIMS2; tick-borne encephalitis virus (TBEV); ZO-1
Received: 2008-11-21
Accepted: 2009-1-28
Published Online: 2009-02-07
Published in Print: 2009-04-01
©2009 by Walter de Gruyter Berlin New York
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Keywords for this article
Dengue virus (DENV);
flavivirus;
PDZ domains;
RIMS2;
tick-borne encephalitis virus (TBEV);
ZO-1
Articles in the same Issue
- Minireview
- Central nervous system: cholesterol turnover, brain development and neurodegeneration
- Protein Structure and Function
- Structure-function relationship of the human antimicrobial peptide LL-37 and LL-37 fragments in the modulation of TLR responses
- Interaction of human heat shock protein 70 with tumor-associated peptides
- Human CYP4Z1 catalyzes the in-chain hydroxylation of lauric acid and myristic acid
- Flavivirus NS5 associates with host-cell proteins zonula occludens-1 (ZO-1) and regulating synaptic membrane exocytosis-2 (RIMS2) via an internal PDZ binding mechanism
- Membranes, Lipids, Glycobiology
- Co-expression of 9-O-acetylated sialoglycoproteins and their binding proteins on lymphoblasts of childhood acute lymphoblastic leukemia: an anti-apoptotic role
- Significance of the cyclic structure and of arginine residues for the antibacterial activity of arenicin-1 and its interaction with phospholipid and lipopolysaccharide model membranes
- Molecular Medicine
- Amidinoanthracyclines – a new group of potential anti-hepatitis C virus compounds
- Proteolysis
- Staphylococcal cysteine protease staphopain B (SspB) induces rapid engulfment of human neutrophils and monocytes by macrophages
- A completed KLK activome profile: investigation of activation profiles of KLK9, 10, and 15
