Interaction of a novel form of Pseudomonas aeruginosa alkaline protease (aeruginolysin) with interleukin-6 and interleukin-8
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Nancy R. Matheson
Abstract
Pseudomonas aeruginosa secretes several proteases considered as important virulence factors. In this report we present data indicating that two key proinflammatory cytokines, interleukin-6 (IL-6) and IL-8, are substrates for pseudolysin (elastase) and aeruginolysin (alkaline protease). While IL-6 was totally digested by both proteases, a long form of IL-8 (IL-8-77) was first rapidly processed into a 72-residue form with enhanced chemokine activity, then very slowly degraded. Interestingly, aeruginolysin bearing two additional residues at the N-terminus (Leu-Lys-aeruginolysin) in the absence of calcium degraded both IL-6 and IL-8-72 far more efficiently than the shorter form of the enzyme.
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©2006 by Walter de Gruyter Berlin New York
Articles in the same Issue
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Articles in the same Issue
- 4th General Meeting of the International Proteolysis Society/International Conference on Protease Inhibitors
- Extracellular granzymes: current perspectives
- Impact of the N-terminal amino acid on targeted protein degradation
- Structural aspects of recently discovered viral deubiquitinating activities
- Cysteine cathepsins and caspases in silicosis
- The proprotein convertases and their implication in sterol and/or lipid metabolism
- PREPL: a putative novel oligopeptidase propelled into the limelight
- Human cathepsin L rescues the neurodegeneration and lethality in cathepsin B/L double-deficient mice
- Helicobacter pylori-induced downregulation of the secretory leukocyte protease inhibitor (SLPI) in gastric epithelial cell lines and its functional relevance for H. pylori-mediated diseases
- Increased local levels of granulocyte colony-stimulating factor are associated with the beneficial effect of pre-elafin (SKALP/trappin-2/WAP3) in experimental emphysema
- Interaction of a novel form of Pseudomonas aeruginosa alkaline protease (aeruginolysin) with interleukin-6 and interleukin-8
- Analysis of aldosterone-induced differential receptor-independent protein patterns using 2D-electrophoresis and mass spectrometry
- Modeling the 3D structure of wheat subtilisin/chymotrypsin inhibitor (WSCI). Probing the reactive site with two susceptible proteinases by time-course analysis and molecular dynamics simulations
- A stable analogue of glucose-dependent insulinotropic polypeptide, GIP(LysPAL16), enhances functional differentiation of mouse embryonic stem cells into cells expressing islet-specific genes and hormones
- Transcription factor FOXM1c is repressed by RB and activated by cyclin D1/Cdk4
- Despite its strong transactivation domain, transcription factor FOXM1c is kept almost inactive by two different inhibitory domains
- Inhibition of calcineurin by infusion of CsA causes hyperphosphorylation of tau and is accompanied by abnormal behavior in mice
- Isolation and properties of extracellular proteinases of Penicillium marneffei
- Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL® phage display library
