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Molecular identification and pharmacological profile of the bovine kinin B1 receptor

  • Guillaume Morissette and François Marceau
Published/Copyright: February 9, 2006
Biological Chemistry
From the journal Volume 387 Issue 2

Abstract

To support the study of kinin pharmacology in bovine models of cultured endothelial cells (ECs), the Bovine Genome Project was searched for a B1 receptor (B1R) gene ortholog (BDKRB1). A contig complementary to an intronless coding nucleotide sequence was found. The sequence was amplified from bovine EC DNA, further cloned into pcDNA3 and expressed in COS-1 cells. The bovine B1R sequence was confirmed and extended. A putative Zn2+-binding motif HEXXH is not present (replaced by HDAWP). The receptor binds [3H]Lys-des-Arg9-bradykinin in a saturable manner (Kd 0.36 nM) and exhibits a pharmacological profile similar to that of human B1R.

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Published Online: 2006-02-09
Published in Print: 2006-02-01

©2006 by Walter de Gruyter Berlin New York

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