The structure of the juvenile hormone binding protein gene from Galleria mellonella
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Agnieszka J. Sok
Abstract
Juvenile hormone (JH) and ecdysone are the key hormones controlling insect growth and development. The juvenile hormone binding protein (JHBP) is the first member in the array of proteins participating in JH signal transmission. In the present report a whole jhbp gene sequence (9790 bp) is described. The jhbp gene contains four introns (A–D). All the introns have common flanking sequences: GT at the 5′ and AG at the 3′ end. The first intron is in phase 1, the second in phase 2, and the third and fourth in phase 1. An analysis of these sequences suggests that U2-class spliceosomes are involved in intron excision from pre-mRNA. Several horizontally transmitted elements from other genes were found in the introns. All jhbp exons are positioned in local AT-reach regions of the gene. A search for core promoter regulatory elements revealed that the TATA box starts 29 bp preceding the start of transcription; the sequence TCAGTA representing a putative initiator sequence (Inr) starts at position +14. Eight characteristic sequences for binding Broad-Complex gene products, which coordinate the ecdysone temporal response, are present in the non-coding sequence of the jhbp gene. An analysis of exon locations and intron phases indicates that jhbp gene organization is related to the retinol binding protein gene, a member of the lipocalin family.
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Artikel in diesem Heft
- The structure of the juvenile hormone binding protein gene from Galleria mellonella
- Interaction of thiamin diphosphate with phosphorylated and dephosphorylated mammalian pyruvate dehydrogenase complex
- Purification and characterization of a chitinase-like antifungal protein from black turtle bean with stimulatory effect on nitric oxide production by macrophages
- Nitric oxide covalently labels a 6-hydroxydopa-derived free radical intermediate in the catalytic cycle of copper/quinone-containing amine oxidase from lentil seedlings
- Plasmodium falciparum glutaredoxin-like proteins
- Characterization of the glyoxalases of the malarial parasite Plasmodium falciparum and comparison with their human counterparts
- Transcriptional down-regulation of neurotrophin-3 in chronic obstructive pulmonary disease
- Limb girdle muscular dystrophy in a sibling pair with a homozygous Ser606Leu mutation in the alternatively spliced IS2 region of calpain 3
- GRIP1 mediates the interaction between the amino- and carboxyl-termini of the androgen receptor
- Dual concentration-dependent activity of thyroglobulin type-1 domain of testican: specific inhibitor and substrate of cathepsin L
- Proteolysis of insulin-like growth factor binding proteins (IGFBPs) by calpain
