Purification and characterization of a chitinase-like antifungal protein from black turtle bean with stimulatory effect on nitric oxide production by macrophages
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Kin Tak Chu
Abstract
An antifungal protein was isolated from black turtle bean, Phaseolus vulgaris cv. ‘Black Turtle’. The purified protein displayed an N-terminal amino acid sequence with 60–80% homology to chitinases. The isolated protein specifically inhibited two species of fungi, namely Fusarium oxysporum and Mycosphaerella arachidicola, among several phytopathogenic fungi tested. Its antifungal activity was retained after incubation at 60°C for 15 minutes, diminished after exposure to 80°C, and was completely abrogated after treatment at 100°C. The antifungal protein had higher thermostability than the antifungal protein mollisin from chestnut. It stimulated nitric oxide production by murine peritoneal macrophages, but exerted neither mitogenic nor cytotoxic activity on murine splenocytes, activities that may be related to allergic responses.
References
Beninger, C.W., and Hosfield, G.L. (2003). Antioxidant activity of extracts, condensed tannin fractions, and pure flavonoids from Phaseolus vulgaris L. seed coat color genotypes. J. Agric. Food Chem.51, 7879–7883.10.1021/jf0304324Search in Google Scholar
Bogdan, C. (2001). Nitric oxide and the immune response. Nat. Immunol.2, 907–916.10.1038/ni1001-907Search in Google Scholar
Chu, K.T., and Ng, T.B. (2003). Mollisin, an antifungal protein from the chestnut Castanea mollissima. Planta Med.69, 809–813.Search in Google Scholar
Di Rosa, M., Radomski, M., Carnuccio, R., and Moncada, S. (1990). Glucocorticoids inhibit the induction of nitric oxide synthase in macrophages. Biochem. Biophys. Res. Commun.172, 1246–1252.10.1016/0006-291X(90)91583-ESearch in Google Scholar
Diaz-Perales, A., Collada, C., Blanco, C., Sanchez-Monge, R., Carrillo, T., Aragoncillo, C., and Salcedo, G. (1998). Class I chitinases with hevein-like domain, but not class II enzymes, are relevant chestnut and avocado allergens. J. Allergy Clin. Immunol.102, 127–133.10.1016/S0091-6749(98)70063-6Search in Google Scholar
Diaz-Perales, A., Blanco, C., Sanchez-Monge, R., Varela, J., Carrillo, T., and Salcedo, G. (2003). Analysis of avocado allergen (Prs a1) IgE-binding peptides generated by simulated gastric fluid digestion. J. Allergy Clin. Immunol.112, 1002–1007.10.1016/j.jaci.2003.07.006Search in Google Scholar
Gozia, O., Ciopraga, J., Bentia, T., Lungu, M., Zamfirescu, I., Tudor, R., Roseanu, A., and Nitu, F. (1993). Antifungal properties of lectin and new chitinases from potato tubers. C.R. Acad. Sci. III316, 788–792.Search in Google Scholar
Laemmli, U.K., and Favre, M. (1973). Maturation of the head of bacteriophage T4. I. DNA packaging events. J. Mol. Biol.80, 575–599.Search in Google Scholar
Liu, W.K., Ng, T.B., Sze, S.F., and Tsui, K.W. (1993). Activation of peritoneal macrophages by polysaccharopeptide from the mushroom, Coriolus versicolor. Immunopharmacology26, 139–146.10.1016/0162-3109(93)90006-CSearch in Google Scholar
Ng, T.B., and Ye, X.Y. (2003). Fabin, a novel calcyon-like and glucanase-like protein with mitogenic, antifungal and translation-inhibitory activities from broad beans. Biol. Chem.384, 811–815.10.1515/BC.2003.090Search in Google Scholar
Pari, L., and Venkateswaran, S. (2003). Effect of an aqueous extract of Phaseolus vulgaris on plasma insulin and hepatic key enzymes of glucose metabolism in experimental diabetes. Pharmazie58, 916–919.Search in Google Scholar
Pastorello, E.A., Farioli, L., Pravettoni, V., Ortolani, C., Fortunato, D., Giuffrida, M.G., Perono Garoffo, L., Calamari, A.M., Brenna, O., and Conti, A. (2003). Identification of grape and wine allergens as an endochitinase 4, a lipid-transfer protein, and a thaumatin. J. Allergy Clin. Immunol.111, 350–359.10.1067/mai.2003.35Search in Google Scholar
Peake, H.L., Currie, A.J., Stewart, G.A., and McWilliam, A.S. (2003). Nitric oxide production by alveolar macrophages in response to house dust mite fecal pellets and the mite allergens, Der p1 and Der p2. J. Allergy Clin. Immunol.112, 531–537.10.1016/S0091-6749(03)01626-9Search in Google Scholar
Ponstein, A.S., Bres-Vloemans, S.A., Sela-Buurlage, M.B., van den Elzen, P.J., Melchers, L.S. and Cornelissen, B.J. (1994). A novel pathogen- and wound-inducible tobacco (Nicotiana tabacum) protein with antifungal activity. Plant Physiol.104, 109–118.10.1104/pp.104.1.109Search in Google Scholar PubMed PubMed Central
Rees, DD, Palmer, R.M.J., Hodson, H.F., and Moncada, S. (1989). A specific inhibitor of nitric oxide formation from l-arginine attenuates endothelium-dependent relaxation. Br. J. Pharmacol.96, 418–423.10.1111/j.1476-5381.1989.tb11833.xSearch in Google Scholar
Tashiro, M., Suzuki, F., Shirataki, Y., Yokote, Y., Akahane, K., Motohashi, N., Ishihara, M., Satoh, K., and Sakagami, H. (2002). Effects of isoflavones from Sophora species on the growth and activation of a mouse macrophage-like cell line. Anticancer Res.22, 2185–2191.Search in Google Scholar
Vogelsang, R., and Barz, W. (1993). Purification, characterization and differential hormonal regulation of a β-1,3-glucanase and two chitinases from chickpea (Cicer arietinum L.). Planta189, 60–69.10.1007/978-94-011-1737-1_96Search in Google Scholar
Wang, H., Ye, X.Y., and Ng, T.B. (2001). Purification of chrysancorin, a novel antifungal protein with mitogenic activity from garland chrysanthemum seeds. Biol. Chem.382, 947–951.10.1515/BC.2001.118Search in Google Scholar
Ye, X.Y., and Ng, T.B. (2000). Mungin, a novel cyclophilin-like antifungal protein from the mung bean. Biochem. Biophys. Res. Commun.273, 1111–1115.10.1006/bbrc.2000.3067Search in Google Scholar
Ye, X.Y., and Ng, T.B. (2002). Isolation of a new cyclophilin-like protein from chickpeas with mitogenic, antifungal and anti-HIV-1 reverse transcriptase activities. Life Sci.70, 1129–1138.10.1016/S0024-3205(01)01473-4Search in Google Scholar
Ye, X.Y., and Ng, T.B. (2003). Isolation of pisumin, a novel antifungal protein from legumes of the sugar snap pea Pisum sativum var. macrocarpon. Comp. Biochem. Physiol. C Toxicol. Pharmacol.134, 235–240.10.1016/S1532-0456(02)00251-XSearch in Google Scholar
Ye, X.Y., Wang, H.X., and Ng, T.B. (2000). Dolichin, a new chitinase-like antifungal protein isolated from field beans (Dolichos lablab). Biochem. Biophys. Res. Commun.269, 155–159.10.1006/bbrc.2000.2115Search in Google Scholar PubMed
Ye, X.Y., Ng, T.B., and Rao, P.F. (2001). A Bowman-Birk-type trypsin-chymotrypsin inhibitor from broad beans. Biochem. Biophys. Res. Commun.289, 91–96.10.1006/bbrc.2001.5965Search in Google Scholar PubMed
©2004 by Walter de Gruyter Berlin New York
Articles in the same Issue
- The structure of the juvenile hormone binding protein gene from Galleria mellonella
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- Purification and characterization of a chitinase-like antifungal protein from black turtle bean with stimulatory effect on nitric oxide production by macrophages
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Articles in the same Issue
- The structure of the juvenile hormone binding protein gene from Galleria mellonella
- Interaction of thiamin diphosphate with phosphorylated and dephosphorylated mammalian pyruvate dehydrogenase complex
- Purification and characterization of a chitinase-like antifungal protein from black turtle bean with stimulatory effect on nitric oxide production by macrophages
- Nitric oxide covalently labels a 6-hydroxydopa-derived free radical intermediate in the catalytic cycle of copper/quinone-containing amine oxidase from lentil seedlings
- Plasmodium falciparum glutaredoxin-like proteins
- Characterization of the glyoxalases of the malarial parasite Plasmodium falciparum and comparison with their human counterparts
- Transcriptional down-regulation of neurotrophin-3 in chronic obstructive pulmonary disease
- Limb girdle muscular dystrophy in a sibling pair with a homozygous Ser606Leu mutation in the alternatively spliced IS2 region of calpain 3
- GRIP1 mediates the interaction between the amino- and carboxyl-termini of the androgen receptor
- Dual concentration-dependent activity of thyroglobulin type-1 domain of testican: specific inhibitor and substrate of cathepsin L
- Proteolysis of insulin-like growth factor binding proteins (IGFBPs) by calpain