Identification of a Gephyrin-Binding Motif in the GDP/GTP Exchange Factor Collybistin
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Y. Grosskreutz
Abstract
The brainspecific GDP/GTP exchange factor collybistin interacts with the receptoranchoring protein gephyrin and activates the Rholike GTPase Cdc42, which is known to regulate actin cytoskeleton dynamics. Alternative splicing creates two collybistin variants, I and II. In coexpression experiments, collybistin II has been shown to induce the formation of submembraneous gephyrin aggregates which cluster with heterooligomeric glycine receptors (GlyRs). Here we identified residues critical for interaction with gephyrin in the linker region between the SH3 and the DH domains of collybistin. Respective collybistin deletion mutants failed to bind gephyrin upon coexpression in heterologous cells, in GST pulldown assays and in the yeast twohybrid system. Sitedirected mutagenesis revealed polar amino acid residues as essential determinants of gephyrin binding. Furthermore, in vitro gephyrin bound simultaneously to both collybistin and the GlyRβsubunit binding motif. Our data are consistent with collybistingephyrin interactions occuring during inhibitory postsynaptic membrane formation.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Protein-DNA Interaction and CpG Methylation at rep*/vIL-10p of Latent Epstein-Barr Virus Genomes in Lymphoid Cell Lines
- In Vitro and in Vivo Processing of Cyanelle tmRNA by RNase P
- A Eubacterial Origin for the Human tRNA Nucleotidyltransferase?
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- The MKK6/p38 Mitogen-Activated Protein Kinase Pathway Is Capable of Inducing SOCS3 Gene Expression and Inhibits IL-6-Induced Transcription
- Identification of a Gephyrin-Binding Motif in the GDP/GTP Exchange Factor Collybistin
- Major Vault Protein Is a Substrate of Endogenous Protein Kinases in CHO and PC12 Cells
- Identification of Receptors for Prothymosin α on Human Lymphocytes
- Tissue Kallikrein KLK1 Is Expressed de Novo in Endothelial Cells and Mediates Relaxation of Human Umbilical Veins
- Secretion of Gelatinases and Activation of Gelatinase A (MMP-2) by Human Rheumatoid Synovial Fibroblasts
- Ferredoxins from the Archaeon Acidianus ambivalens: Overexpression and Characterization of the Non-Zinc-Containing Ferredoxin FdB
- Isolation and Characterization of a Trypsin-Like Protease from Trichoderma viride
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