Protein-DNA Interaction and CpG Methylation at rep*/vIL-10p of Latent Epstein-Barr Virus Genomes in Lymphoid Cell Lines
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H.H. Niller
Abstract
The viral interleukin-10 promoter (vIL-10p), overlapping the rep* element in the EpsteinBarr virus (EBV) genome, is a promoter element active mostly in the late phase of the lytic cycle and immediately upon infection of B cells. rep* was, through transfection experiments with small plasmids, characterised as a cis element supporting oriP replicative function. In this study, in vivo protein binding and CpG methylation at rep*/vIL-10p were analysed in five cell lines that harbour strictly latent EBV genomes. Contrary to the invariably unmethylated dyad symmetry element (DS) of oriP, rep*/vIL-10p was highly methylated and showed only traces of protein binding in all examined cell lines. This result is in agreement with vIL-10p being an inactive promoter of EBV genomes, and makes it less likely that rep* functions as a replicative element of latent EBV genomes.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Protein-DNA Interaction and CpG Methylation at rep*/vIL-10p of Latent Epstein-Barr Virus Genomes in Lymphoid Cell Lines
- In Vitro and in Vivo Processing of Cyanelle tmRNA by RNase P
- A Eubacterial Origin for the Human tRNA Nucleotidyltransferase?
- Interaction of Thymidylate Synthase with the 5-Thiophosphates, 5-Dithiophosphates, 5-H-Phosphonates and 5-S-Thiosulfates of 2-Deoxyuridine, Thymidine and 5-Fluoro-2-Deoxyuridine
- The MKK6/p38 Mitogen-Activated Protein Kinase Pathway Is Capable of Inducing SOCS3 Gene Expression and Inhibits IL-6-Induced Transcription
- Identification of a Gephyrin-Binding Motif in the GDP/GTP Exchange Factor Collybistin
- Major Vault Protein Is a Substrate of Endogenous Protein Kinases in CHO and PC12 Cells
- Identification of Receptors for Prothymosin α on Human Lymphocytes
- Tissue Kallikrein KLK1 Is Expressed de Novo in Endothelial Cells and Mediates Relaxation of Human Umbilical Veins
- Secretion of Gelatinases and Activation of Gelatinase A (MMP-2) by Human Rheumatoid Synovial Fibroblasts
- Ferredoxins from the Archaeon Acidianus ambivalens: Overexpression and Characterization of the Non-Zinc-Containing Ferredoxin FdB
- Isolation and Characterization of a Trypsin-Like Protease from Trichoderma viride
- The N-Terminal Part of Recombinant Human Tear Lipocalin/von Ebners Gland Protein Confers Cysteine Proteinase Inhibition Depending on the Presence of the Entire Cystatin-Like Sequence Motifs
