The MKK6/p38 Mitogen-Activated Protein Kinase Pathway Is Capable of Inducing SOCS3 Gene Expression and Inhibits IL-6-Induced Transcription
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J.G. Bode
Abstract
In this study we show that activation of p38MAPKby IL-6 acts as an inhibitory signal on IL-6-mediated activation of STAT and the α2-macroglobulin promoter. We analyzed the role of MKK6/p38MAPKfor IL-6 signal transduction and transcriptional activation of the suppressor of cytokine signaling (SOCS) 3 promoter. Pretreatment of cells with the p38MAPKspecific inhibitor SB202190 downregulates the induction of SOCS3-mRNA expression by IL-6. Accordingly, overexpression of a constitutively active MKK6 in HepG2 cells enhanced basal activity or IL-6-induced transcriptional activation of a SOCS3 promoter reporter construct, whereas overexpression of a dominant negative mutant of MKK6 downregulated the IL-6-mediated activation of the SOCS3 promoter. These data indicate that p38MAPKactivation is crucial for IL-6-induced SOCS3 expression and downregulation of IL-6-mediated gene induction.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Protein-DNA Interaction and CpG Methylation at rep*/vIL-10p of Latent Epstein-Barr Virus Genomes in Lymphoid Cell Lines
- In Vitro and in Vivo Processing of Cyanelle tmRNA by RNase P
- A Eubacterial Origin for the Human tRNA Nucleotidyltransferase?
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- The MKK6/p38 Mitogen-Activated Protein Kinase Pathway Is Capable of Inducing SOCS3 Gene Expression and Inhibits IL-6-Induced Transcription
- Identification of a Gephyrin-Binding Motif in the GDP/GTP Exchange Factor Collybistin
- Major Vault Protein Is a Substrate of Endogenous Protein Kinases in CHO and PC12 Cells
- Identification of Receptors for Prothymosin α on Human Lymphocytes
- Tissue Kallikrein KLK1 Is Expressed de Novo in Endothelial Cells and Mediates Relaxation of Human Umbilical Veins
- Secretion of Gelatinases and Activation of Gelatinase A (MMP-2) by Human Rheumatoid Synovial Fibroblasts
- Ferredoxins from the Archaeon Acidianus ambivalens: Overexpression and Characterization of the Non-Zinc-Containing Ferredoxin FdB
- Isolation and Characterization of a Trypsin-Like Protease from Trichoderma viride
- The N-Terminal Part of Recombinant Human Tear Lipocalin/von Ebners Gland Protein Confers Cysteine Proteinase Inhibition Depending on the Presence of the Entire Cystatin-Like Sequence Motifs
