Complement Factor I Is Upregulated in Rat Hepatocytes by Interleukin-6 But Not by Interferon-γ , Interleukin-1β or Tumor Necrosis Factor-α
-
Gerald Schlaf
Abstract
Complement factor I (FI) is a regulatory serine protease of the complement system which cleaves three peptide bonds in the αchain of C3b and two bonds in the αchain of C4b and thus prevents the assembly of the C3 and C5 convertases. We have investigated the proinflammatory cytokines IL-6, IL-1β, TNFα and IFNγ for their potential role in the regulation of FI expression. Of the investigated cytokines, only IL-6 increased the FIspecific RTPCR signal in isolated hepatocytes, in the two rat hepatomaderived cell lines FAO and H4IIE or in HUVECs. Quantitative competitive RTPCR showed an IL-6 induced upregulation of FIspecific mRNA by about tenfold. These data are in accord with Northern blot analyses in which the FImRNA was upregulated by IL-6 between five and sevenfold. IL-6, but not IL-1β, TNFα or IFNγ also increased FIprotein levels in cell culture supernatants by about fivefold as determined by a semiquantitative immunoblot using a novel monoclonal antibody specific for rat FI.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Functional Genomics in HIV-1 Virus Replication: Protein-Protein Interactions as a Basis for Recruiting the Host Cell Machinery for Viral Propagation
- On the Characterization of the Putative S20-Thx Operon of Thermus thermophilus
- RNase-Stable RNA: Conformational Parameters of the Nucleic Acid Backbone for Binding to RNase T1
- Enhanced Gamma-Glutamyl Transpeptidase Expression and Superoxide Production in Mpv17-/-Glomerulosclerosis Mice
- Juvenile Hormone Binding Protein and Transferrin from Galleria mellonella Share a Similar Structural Motif
- Analysis of Structural Signals Conferring Localisation of Pig OST48 to the Endoplasmic Reticulum
- Identification and Crystallisation of a Heat- and Protease-Stable Fragment of the Bacteriophage T4 Short Tail Fibre
- Insulin Resistance Induced by Loop Diuretics and Hyperosmolarity in Perfused Rat Liver
- Singlet Molecular Oxygen Triggers the soxRS Regulon of Escherichia coli
- The Extracellular Signal-Regulated Protein Kinases Erk1/Erk2 Stimulate Expression and Biological Activity of the Transcriptional Regulator Egr-1
- The FIRE3-Mediated Sterol Response of the FAS Promoter Requires NF-Y/CBF as a Coactivator
- Complement Factor I Is Upregulated in Rat Hepatocytes by Interleukin-6 But Not by Interferon-γ , Interleukin-1β or Tumor Necrosis Factor-α
- Isolation and Characterization of a Highly Specific Serine Endopeptidase from an Oral Strain of Staphylococcus epidermidis
Articles in the same Issue
- Functional Genomics in HIV-1 Virus Replication: Protein-Protein Interactions as a Basis for Recruiting the Host Cell Machinery for Viral Propagation
- On the Characterization of the Putative S20-Thx Operon of Thermus thermophilus
- RNase-Stable RNA: Conformational Parameters of the Nucleic Acid Backbone for Binding to RNase T1
- Enhanced Gamma-Glutamyl Transpeptidase Expression and Superoxide Production in Mpv17-/-Glomerulosclerosis Mice
- Juvenile Hormone Binding Protein and Transferrin from Galleria mellonella Share a Similar Structural Motif
- Analysis of Structural Signals Conferring Localisation of Pig OST48 to the Endoplasmic Reticulum
- Identification and Crystallisation of a Heat- and Protease-Stable Fragment of the Bacteriophage T4 Short Tail Fibre
- Insulin Resistance Induced by Loop Diuretics and Hyperosmolarity in Perfused Rat Liver
- Singlet Molecular Oxygen Triggers the soxRS Regulon of Escherichia coli
- The Extracellular Signal-Regulated Protein Kinases Erk1/Erk2 Stimulate Expression and Biological Activity of the Transcriptional Regulator Egr-1
- The FIRE3-Mediated Sterol Response of the FAS Promoter Requires NF-Y/CBF as a Coactivator
- Complement Factor I Is Upregulated in Rat Hepatocytes by Interleukin-6 But Not by Interferon-γ , Interleukin-1β or Tumor Necrosis Factor-α
- Isolation and Characterization of a Highly Specific Serine Endopeptidase from an Oral Strain of Staphylococcus epidermidis
