The FIRE3-Mediated Sterol Response of the FAS Promoter Requires NF-Y/CBF as a Coactivator
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Abstract
The transcription of the fatty acid synthase (FAS) gene is regulated by the sterol status of the cell via cleavage of the sterol regulatory elementbinding protein (SREBP). When human HepG2 hepatoma cells were cotransfected with an expression plasmid for mature SREBP-1a together with FAS promoter/reporter constructs significant increases in reporter activity were observed. Deletion analysis of the FAS promoter between 151 and 52 relative to the transcription start site pinpoint two ciselements important in sterol regulation of the FAS gene. One element, FIRE3, between 71 and 52 can bind in vitro translated and transcribed SREBP-1a whereas the other element, the inverted CCAAT element ICE( 97/ 92), binds the trimeric transcription factor NFY/CBF as shown with rat liver extract and reconstituted, recombinant NFY. The results clearly show that the coactivator for SREBP-1a in this cell line is NFY. This finding was confirmed by using a dominant negative form of NFYA, NFYAm29, which interferes with the effect of ectopically expressed SREBP-1a on FAS reporter activity.
Copyright © 2001 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- Functional Genomics in HIV-1 Virus Replication: Protein-Protein Interactions as a Basis for Recruiting the Host Cell Machinery for Viral Propagation
- On the Characterization of the Putative S20-Thx Operon of Thermus thermophilus
- RNase-Stable RNA: Conformational Parameters of the Nucleic Acid Backbone for Binding to RNase T1
- Enhanced Gamma-Glutamyl Transpeptidase Expression and Superoxide Production in Mpv17-/-Glomerulosclerosis Mice
- Juvenile Hormone Binding Protein and Transferrin from Galleria mellonella Share a Similar Structural Motif
- Analysis of Structural Signals Conferring Localisation of Pig OST48 to the Endoplasmic Reticulum
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- Singlet Molecular Oxygen Triggers the soxRS Regulon of Escherichia coli
- The Extracellular Signal-Regulated Protein Kinases Erk1/Erk2 Stimulate Expression and Biological Activity of the Transcriptional Regulator Egr-1
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- Complement Factor I Is Upregulated in Rat Hepatocytes by Interleukin-6 But Not by Interferon-γ , Interleukin-1β or Tumor Necrosis Factor-α
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