Startseite Do Rodent and Human Brains Have Different N-Glycosylation Patterns?
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

Do Rodent and Human Brains Have Different N-Glycosylation Patterns?

  • Claus Albach , Roger A. Klein und Brigitte Schmitz
Veröffentlicht/Copyright: 1. Juni 2005
Biological Chemistry
Aus der Zeitschrift Band 382 Heft 2

Abstract

A large number of studies on the structure of Nglycosidically linked oligosaccharides from glycoproteins of different organs and/or different species have been carried out in the past using various combinations of techniques such as monosaccharide analysis, permethylation, peracteylation, exoglycosidase sequencing, normal and reversed phase HPLC, mass spectrometry and nuclear magnetic resonance spectroscopy. Although it is widely accepted that the processing of Nglycans in the ER and Golgi of mammalian cells follows the same principal metabolic rules, analyses have revealed that the glycosylation pattern of a particular protein may differ depending on the cell type in which it is expressed. Nglycans from brain glycoproteins have been shown to include a variety of hybrid and complextype structures with structural features that are not so commonly found on glycoproteins from other organs and which have, therefore, been classified as brainspecific. Comparison of the Nglycans of glycoproteins from homogenates of rat, mouse and human brains confirm that, in general, glycoproteins from human brain show a similar profile of brainspecific Nglycans as glycoproteins from mouse and rat brain.

:
Published Online: 2005-06-01
Published in Print: 2001-02-12

Copyright © 2001 by Walter de Gruyter GmbH & Co. KG

Artikel in diesem Heft

  1. Highlight: Glycobiology
  2. O-Glycosylation of the Mucin Type
  3. Glycoproteins from Insect Cells: Sialylated or Not?
  4. Congenital Disorders of Glycosylation: Glycosylation Defects in Man and Biological Models for Their Study
  5. Mitochondrial Single-Stranded DNA-Binding Proteins: in Search for New Functions
  6. Do Rodent and Human Brains Have Different N-Glycosylation Patterns?
  7. The Liver Flukes Fasciola gigantica and Fasciola hepatica Express the Leucocyte Cluster of Differentiation Marker CD77 (Globotriaosylceramide) in Their Tegument
  8. Cloning and Expression of Drosophila melanogaster UDP-GlcNAc:?-3-D-Mannoside ? 1,2-N-Acetylglucosaminyltransferase I
  9. Pathways of Mucin O-Glycosylation in Normal and Malignant Rat Colonic Epithelial Cells Reveal a Mechanism for Cancer-Associated Sialyl-Tn Antigen Expression
  10. 6-O-Sulfo De-N-Acetylsialyl Lewis X as a Novel High-Affinity Ligand for Human L-Selectin: Total Synthesis and Structural Characterization
  11. Segregation of Gangliosides GM1 and GD3 on Cell Membranes, Isolated Membrane Rafts, and Defined Supported Lipid Monolayers
  12. Structural Characterization of Fucose-Containing Oligosaccharides by High-Performance Liquid Chromatography and Matrix-Assisted Laser Desorption/ Ionization Time-of-Flight Mass Spectrometry
  13. Anencephaly: Structural Characterization of Gangliosides in Defined Brain Regions
  14. Acidic Glycerol Lipids of Trichomonas vaginalis and Tritrichomonas foetus
  15. Stimulation of Acid Sphingomyelinase Activity by Lysosomal Lipids and Sphingolipid Activator Proteins
  16. Biosynthesis of N-Acetylneuraminic Acid in Cells Lacking UDP-N-Acetylglucosamine 2-Epimerase/ N-Acetylmannosamine Kinase
  17. Synthesis of Nucleotide-Activated Oligosaccharides by ?-Galactosidase from Bacillus circulans
  18. Elucidation of the Role of Functional Amino Acid Residues of the Small Sialidase from Clostridium perfringens by Site-Directed Mutagenesis
  19. Biosynthesis of Lipid-Linked Oligosaccharides in Yeast: the ALG3 Gene Encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol Mannosyltransferase
  20. Intra- and Intermolecular Triplex DNA Formation in the Murine c-myb Proto-Oncogene Promoter Are Inhibited by Mithramycin
  21. Experimental Regulation of STAT Gene Expression Reveals an Involvement of STAT5 in Interleukin-4-Driven Cell Proliferation
Heruntergeladen am 21.10.2025 von https://www.degruyterbrill.com/document/doi/10.1515/BC.2001.026/html?lang=de
Button zum nach oben scrollen