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Dequalinium TM Vesicles Form Stable Complexes with Plasmid DNA which Are Protected from DNase Attack

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Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Biological Chemistry Volume 380 Issue 6

Abstract

Upon sonication, the antimicrobial and antineoplastic compound dequaliniumTM forms vesicles (DQAsomes, Weissig et al., 1998). DequaliniumTM (1,1′-(1,10-decamethylene-bis-[aminoquinaldinium])-chloride) was shown to be a fluorophore with an emission maximum at 366 nm. Addition of DNA results in a characteristic quenching of its intrinsic fluorescence. After density gradient centrifugation a band of dequaliniumTM (DQA) tightly associated with DNA is located between the DNA and DQA bands. DQA/DNA-complexes containing plasmid DNA at a molar ratio of DQA/DNA 6:1 are completely protected against DNase activity. Addition of negatively-charged lipids release intact DNA in the same manner as from cationic lipid/DNA complexes. As regards biological effects, DQAsomes show a differential cytotoxicity for normal and sarcoma cell lines. In vitro incubation with fluorescein-labeled oligodeoxynucleotides (5′-fluorescein-[GATC]5) showed an increased uptake of the tagged oligodeoxynucleotide if complexed with dequalinium.

We hypothesize that the DQA/DNA complexes are well-suited for ‘DQAsomal gene transfer’ in vitro and in vivo. Noteworthy, they display an intrinsic antitumor activity manifested by differential cytotoxicity for normal and sarcoma cells.

Published Online: 2005-6-1
Published in Print: 1999-6-1

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

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https://doi.org/10.1515/BC.1999.080

Articles in the same Issue

  1. To Our Authors, Readers and Subscribers
  2. Michael Strauss. January 12, 1950 April 29, 1999
  3. Monoallelic Expression of Pax5: A Paradigm for the Haploinsufficiency of Mammalian Pax Genes?
  4. Adeno-Associated Viral Vectors for Gene Transfer and Gene Therapy
  5. Histidyl-tRNA Synthetase
  6. Dequalinium TM Vesicles Form Stable Complexes with Plasmid DNA which Are Protected from DNase Attack
  7. On the Role of Thymopoietins in Cell Proliferation. Immunochemical Evidence for New Members of the Human Thymopoietin Family
  8. Functional Characterization of Atherosclerosis-Associated Ser128Arg and Leu554Phe E-Selectin Mutations
  9. Cycloheximide, a New Tool to Dissect Specific Steps in ER-Associated Degradation of Different Substrates
  10. Localization of Rat Cathepsin K in Osteoclasts and Resorption Pits: Inhibition of Bone Resorption and Cathepsin K-Activity by Peptidyl Vinyl Sulfones
  11. Oxidation of 4-tert-Butylcatechol and Dopamine by Hydrogen Peroxide Catalysed by Horseradisch Peroxidase
  12. A Non-Radioactive Method for Inexpensive Quantitative RT-PCR
  13. Activation of the Transcription Factor ISGF3 by Interferon-gamma
  14. Acquisition of Myogenic Specificity through Replacement of One Amino Acid of MASH-1 and Introduction of an Additional alpha -Helical Turn
  15. The Heavy Metal-Responsive Transcription Factor-1 (MTF-1) Is Not Required for Neural Differentiation
  16. Human Fc gamma Receptor IIb Expressed in <I>Escherichia coli</I> Reveals IgG Binding Capability
  17. Mutational Analysis of Two Stefin A Epitopes
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