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Use Of In Vitro Culture For Inducing Variation In Rice And Fuchsia
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A. Dekeyser
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Kapitel in diesem Buch
- Frontmatter I
- Preface V
- Acknowledgements VII
- Contents IX
-
Part 1. Use Of Mutation In Breeding Research
- Mutation breeding: a stepping stone between Gregor Mendel and genetic manipulation (a treatise for vegetatively propagated crops) 3
- Induced structural rearrangements 17
- Evaluation and selection of mutations on the basis of their conformity to plant ideotype 37
- Chemically induced mutations for male sterility in Petunia x hybrida 43
- Genome adjustment by breeding to balance yield defects in high-lysine mutants of barley 49
- Selection of rust resistant wheat after mutagenesis 61
- Use of maize mutants in breeding for improvement of protein quality 65
- Promising rice mutants for developed production 69
- Cytoplasmic male sterility in maize - nuclear and mitochondrial genome interdependence 73
- Genetic studies and inheritance of seed storage protein of pea chromosome mutants 77
-
Part 2. Cytogenetics And Polyploidy
- Cytogenetics In Breeding Programmes Dealing With Polyploidy, Interspecific Hybridization And Introgression 83
- Tetraploid triticale - a tool in hexaploid triticale breeding 105
- Instability in tomato lines with the breakage-fusion-bridge cycle 119
- The present status of breeding autotetraploid cereals 123
- Alien chromosome transfer from wheat into rye 129
- Nucleolar competition in different (A/B)(A/B)RR and DDRR genomes of tetraploid triticales 137
- Identification of interchanges in wild species of Pisum 141
- Genetic regulation of meiotic recombination in Petunia hybrida 145
- Attempts to transfer resistance to Phoma lingam from Brassica juncea and B. carinata to B. napus through interspecific hybridization followed by ovule culture 149
- Analysis of ADH1 locus in tetraploid corn /Zea mays L./ 153
- C-banding of Avena species 157
- A study of nucleic acids in the Symphytum officinale species complex and S. asperum: microdensitometry of nuclear DNA in leaf cells 161
- C-banding and isozyme markers to analyze the segregation of rye chromosomes in the progenies of triticale x wheat hybrids 163
- Breeding nematode-resistant sugar beets 167
- Polyploidy in hop breeding, Humulus lupulus L. 171
- Reconstitution of hexaploid wheat from Triticum dicoccoides (AABB, 2n=28) and T. tauschii (DD, 2n=14) 175
- Preferential loss and gain of specific Hordeum vulgare chromosomes in hybrids with three alien species? 179
- Genetic and cytological maps in Petunia 183
- Biochemical characterization of cytoplasmic male sterility in Petunia hybrida 187
- Cytogenetic studies of the polyploids of hop, Humulus lupulus L. 191
- Cytogenetic studies of wheat lines with rye chromosome additions 195
- Substitution of chromosome 5S of Aegilops longissima for its group-5 homoeologues of common wheat 199
- Interspecific hybridization between V. faba and V. narbonensis: prospects and limitations 203
- Alien gene transfer in groundnut by ploidy and genome manipulations (ICRISAT C.P. No. 277) 207
- The potential for improving the primary distant hybrids of common wheat by anther culture 211
-
Part 3. Induction and Use Of Haploids
- Criteria for the selection and use of doubled haploid systems in cereal breeding programmes 217
- Haploid induction and production in crop plants 231
- Effects of activated charcoal, cold treatment and elevated CO2-concentrations on embryogenesis in anther cultures 257
- Utilisation of anther culture in breeding Brussels sprouts 265
- The role of linkage and recombination in barley breeding with Hordeum bulbosum L. 273
- Partial incompatibility between Scandinavian six-rowed barleys and Hordeum bulbosum L. and its genetical basis 275
- Recent results on somatic embryogenesis in pea and bean 279
- Sugar beet (Beta vulgaris L.) pollen quality assessment and effect of irradiation as measured by fluorochromatic reaction and in vitro germination 283
- The detection of linkage using doubled haploids in barley 287
- The Effect Of An Elevated CO2 Concentration In Combination With Cold Treatments In Maize (Zea Mays L.) Anther Culture 291
- Obtention Of Embryos And Plants From In Vitro Culture Of Unfertilized Ovules Of Cucurbita Pepo 295
- Agronomic Value Of Androgenetic Doubled Haploid Lines As Compared To Conventionally Selected Spring Barley 299
- Pollen Plant Production In Triticum Turgidum Ssp.Durum 303
- Production Of Haploid Sugarbeets (Beta Vulgaris L.) By Ovule Culture 307
- Towards The Isolation Of Sperm Cells For Androgenic Purposes 311
- Regeneration And Selection Of Isolated Microspores Of Hordeum Vulgare 315
- Possibilities Of Obtainment And Utilization Of Doubled Haploids In Gerbera 319
- Contribution To The Studies On The First,Generative Progeny Of Kragaria X Ananassa Polyhaploids 323
- A Comparison Of Cross Prediction Methods In Spring Barley 327
- Androgenesis In Oilseed Rape 331
- Production Of Doubled Haploids In Oriental Lilies 335
- Increasing The Efficiency Of Triticale Anther Culture 339
- Genetic Gain For Some Agronomical Characters By Dihaploid Breeding In Barley 343
- Effect Of A Gametocide On The Induction Of Haploids In Triticum Aestivum 347
- The Induction Of Haploids Of Sugarbeet (Beta Vulgaris L.) Using Anther And Free Pollen Culture Or Ovule And Ovary Culture 351
- Frost Tolerant Plants Obtained From Proline Accumulating Cell Lines 355
- Somatic Cell Genetics Of Potato I. Use Of Monohaploids 359
- Factors Affecting Callus And Plant Production In Anther Cultuses Of Tomato 361
-
Part 4. In-Vitro Propagation
- Role Of Methodology In Facilitating Application Of Tissue Culture Techniques 367
- In Vitro Propagation And Breeding Of Ornamental Plants: Advantages And Disadvantages Of Variability 377
- Protoplast Culture And Use Of Regeneration Attributes To Select Somatic Hybrid Tomato Plants 405
- Sexual Reproduction In Plants By Applying The Method Of Test Tube Fertilization Of Ovules 415
- Cytogenetic Studies In Callus Cultures Of Asparagus Off 425
- Systems For Regeneration Of Cucumis Sativus Plants In Vitro 429
- Regeneration Of Temperate Fruit Trees In Vitro Via Organogenesis And Embryogenesis 433
- Problems And Prospects For The Use Of Protoplasts In Beet Breeding 437
- Shoot Redifferentiation Of Agrobacterium Transformed-Protoplasts And Plant Tissue- With Conventional Methods Not Achievable 441
- Induction Of In Vitro-Regeneration Via Somatic Embryogenesis In Pea (Pisum Sativum) And Bean (Phaseolus Vulgaris) 445
- Endogenous Cytokinins During Embryogenesis In A Carrot Cell Suspension 449
- Tissue And Protoplast Culture In Cultivated Beets 453
- Protoplast Formation In Cereals - An Assessment 457
- Exploitation For Breeding Of In Vitro Culture Of Pea Explants 461
- Some Aspects Of The In Vitro Culture Of The Beet. (Beta Vulgaris L.) 465
- Interspecific Hybridization Of Red Clover (Trifolium Pratense L.) With Alsike Clover (Trifolium Hybndum L.) Using In Vitro Embryo Rescue 469
- Pollen And Ovule Cultures Of Barley To Isolate, Manipulate And Transfer Sperm Cells In In Vitro Fertilization 473
- Strategies In High Frequency Regeneration From Diploid And Haploid Cell And Tissue Cultures Of Barley 477
- Somaclonal Variation In Plants Regenerated From Embryo Calluses In Rye (Secale Cerale L.) 479
- Somatic Embryogenesis, Cell And Protoplast Culture Of Hordeum Vulgare L. (Barley) 483
- Isolation And Culture Of Protoplasts From Callus And Suspensioncultured Cells Of Prunus Cerasus And Actinidia Chinensis 487
- Somatic Embryogenesis In Protoplast-Derived Cells Of Cucumis Melo L. 491
- A Comparative Study Of Callus Formation And Plant Regeneration From Different Explants Of Phaseolus Vulgaris And Ph. Coccineus 495
- Somatic Embryogenesis, Cell And Protoplast Culture Of Triticale (X Triticosecale Wittmack) 499
- Somatic Embryogenesis And Plant Regeneration From Meristematic Tissue Of Secale Cereale (Rye) 503
- Somatic Embryogenesis Of Triticale 507
-
Part 5. Spontaneous And Induced Variation From In-Vitro Cultures
- Variability In Tissue Culture Derived Plants - Possible Origins; Advantages And Drawbacks 513
- Screening For Virus Resistance In Tissue Culture Adventitious Regenerants And Their Progeny 535
- Chromosome Variation In Regenerated Plants 547
- In Vitro Mutagenesis In Gerbera Jamesonii 555
- In Vitro Mutagenesis In Maize 563
- Somaclones Of Wheat Regenerated From Primordial Leaf Callus 577
- Somaclonal Variation In Lotus Corniculatus L. 581
- Potential System For The Specific Selection Of Plant Mutants Overproducing Methionine 585
- Enhancement Of Asulam Resistance In Barley 589
- Triazine-Resistant Nicotiana Mutants From Photomixotrophic Cell Cultures 593
- Use Of In Vitro Culture For Inducing Variation In Rice And Fuchsia 597
- Isolation Of Auxotrophic Mutants Based On Reconstruction Experiments With Nicotiana Plumbaginifolia Protoplasts 599
- A Study On The Effect Of Different Initial Culture Media On The Chromosome Stability Of Solanum Tuberosum Cv. Maris Bard Protoplast Derived Regenerants 601
- The Effect Of Explant Source, In Vitro Regeneration And Irradiation On Variation In Yield Induced In Chrysanthemum Morifolium 607
- Transfer Of Genetic Material To Cultivated Barley From Alien Species Through Callus Culture (Preliminary Results) 611
- Somatic Embryogenesis And Plant Regeneration From Leaf Tissues Of Secale Cereale L 615
- Testing Of Salt /Nacl/ Tolerance And Regeneration In Callus Culture /n, 2n/ Of Rice 617
- The Use Of Tissue Cultures For Obtaining Tobacco Male Sterile Forms 621
- Somaclonal Variation In Triticale 625
- Separation, Identification And Biological Effects Of A Toxin Produced By Phoma Lingam 629
- Variability In Plants Of Sugar Beet (Beta Vulgaris L.) Regenerated From Callus, Cell-Suspension And Protoplasts 633
- Chromosomal Variation In Regenerated Plants From Hybrid Callus From Crosses Between Hordeum Vulgare X H. Bulbosum 637
-
Part 6. Somatic Hybridization And Cybridization
- Plant Cell Fusion As A Tool For Genetic Manipulation 641
- Genetic Improvement Of Cytoplasmic Traits Through Cytoplasmic Hybridization In Cruciferae 653
- Somatic Hybridization And Cybridization As Potential Methods For Widening Of The Gene-Pools Of Crops Within Brassicaceae And Solanaceae 663
- Investigations Into The Transfer Of Genetic Information Between Solanaceous Species And Potato By Somatic Hybridization 683
- Protoplast Culture And Fusion Of Red And Alsike Clover 685
- Interclassical Protoplast Fusion Between Orchard-Grass And Petunia 689
- Interspecific Chloroplast Recombination In A Nicotiana Somatic Hybrid 693
- Loss Of Species-Specific Sequences In Somatic Hybrids, Obtained By Fusion Of Nicotiana Tabacum Cnx Protoplasts With Heavily X-Irradiated N. Paniculata Protoplasts 697
- Somatic Cell Genetics Of Potato: Variant Cell Lines And Somatic Hybridization 701
- Somatic Hybridization Between Two Nicotiana Plumbaginifolia Lines And Between Solanum Tuberosum And Phureja Using Electrofusion 703
- Structure And Regulation Of Cytosol And Plastid Specific Isoenzymes In Higher Plants 705
- Resynthesis Of Brassica Napus Via Somatic Hybridization: A Model For Production Of Interspecific Hybrids Within Brassiceae 709
- Protoplast Culture And Plant Regeneration Of Solanum Pennellii And Lycopersicon Esculentum 713
- Somatic Cell Genetics Of Potato III: Electrofusion Of Two Amino Acid Analogue-Resistant Cell Lines 715
- Cp And Mt Genome Constitution Of Different Somatic Hybrids Between Brassica Napus And Brassica Hirta 719
-
Part 7. Isolation And Cloning Of Plant Genes
- The Complexity Of Gene Regulation During The Lightdependent Development Of Chloroplasts In Barley (Hordeum Vulgare L.) 725
- The Chloroplast Ribosomes Of Higher Plants And The Genes For Their Protein Components 731
- Genetic Variation And Gene Expression; A Study Of The Ribosomal Rna Gene Loci Of Wheat 745
- Cloning And Analysis Of Two Genes For Chalcone Synthase Of Petunia Hybrida 753
- Cm-Proteins And Thionins In Cereals: Characterization And Cloning Of Cdna 763
- Cin-Dna-Insertions Of Zea Mays 771
-
Part 8. Transformation
- Recent Progress In Plant Genetic Engineering 775
- Organ-Specific Gene Expression In Potato 777
- Electric Field Mediated Transfer Of Nucleic Acids Into Carrot Protoplasts 785
- The Pollen System Of Gene Transfer 803
- Direct Gene Transfer To Plants 813
- Genetic Manipulation In Potato 823
- Culture And Transformation Studies With Maize Protoplasts 827
- Transformation In The Forage Legumes 831
-
Part 9. Use Of Molecular Biology Methods For Plant Improvement
- Integration, Expression And Stable Transmission Through Seeds Of Foreign Genes In Plants 837
- Identification, Cloning And Transfer Of Chloroplast Genes Of Petunia Hibrida 859
- The Need For A Multidisciplinary Approach To Genetic Manipulation In Plant Breeding 867
- Appendix 883
- Author Index 895
- Subject Index 899
- Backmatter 910
Kapitel in diesem Buch
- Frontmatter I
- Preface V
- Acknowledgements VII
- Contents IX
-
Part 1. Use Of Mutation In Breeding Research
- Mutation breeding: a stepping stone between Gregor Mendel and genetic manipulation (a treatise for vegetatively propagated crops) 3
- Induced structural rearrangements 17
- Evaluation and selection of mutations on the basis of their conformity to plant ideotype 37
- Chemically induced mutations for male sterility in Petunia x hybrida 43
- Genome adjustment by breeding to balance yield defects in high-lysine mutants of barley 49
- Selection of rust resistant wheat after mutagenesis 61
- Use of maize mutants in breeding for improvement of protein quality 65
- Promising rice mutants for developed production 69
- Cytoplasmic male sterility in maize - nuclear and mitochondrial genome interdependence 73
- Genetic studies and inheritance of seed storage protein of pea chromosome mutants 77
-
Part 2. Cytogenetics And Polyploidy
- Cytogenetics In Breeding Programmes Dealing With Polyploidy, Interspecific Hybridization And Introgression 83
- Tetraploid triticale - a tool in hexaploid triticale breeding 105
- Instability in tomato lines with the breakage-fusion-bridge cycle 119
- The present status of breeding autotetraploid cereals 123
- Alien chromosome transfer from wheat into rye 129
- Nucleolar competition in different (A/B)(A/B)RR and DDRR genomes of tetraploid triticales 137
- Identification of interchanges in wild species of Pisum 141
- Genetic regulation of meiotic recombination in Petunia hybrida 145
- Attempts to transfer resistance to Phoma lingam from Brassica juncea and B. carinata to B. napus through interspecific hybridization followed by ovule culture 149
- Analysis of ADH1 locus in tetraploid corn /Zea mays L./ 153
- C-banding of Avena species 157
- A study of nucleic acids in the Symphytum officinale species complex and S. asperum: microdensitometry of nuclear DNA in leaf cells 161
- C-banding and isozyme markers to analyze the segregation of rye chromosomes in the progenies of triticale x wheat hybrids 163
- Breeding nematode-resistant sugar beets 167
- Polyploidy in hop breeding, Humulus lupulus L. 171
- Reconstitution of hexaploid wheat from Triticum dicoccoides (AABB, 2n=28) and T. tauschii (DD, 2n=14) 175
- Preferential loss and gain of specific Hordeum vulgare chromosomes in hybrids with three alien species? 179
- Genetic and cytological maps in Petunia 183
- Biochemical characterization of cytoplasmic male sterility in Petunia hybrida 187
- Cytogenetic studies of the polyploids of hop, Humulus lupulus L. 191
- Cytogenetic studies of wheat lines with rye chromosome additions 195
- Substitution of chromosome 5S of Aegilops longissima for its group-5 homoeologues of common wheat 199
- Interspecific hybridization between V. faba and V. narbonensis: prospects and limitations 203
- Alien gene transfer in groundnut by ploidy and genome manipulations (ICRISAT C.P. No. 277) 207
- The potential for improving the primary distant hybrids of common wheat by anther culture 211
-
Part 3. Induction and Use Of Haploids
- Criteria for the selection and use of doubled haploid systems in cereal breeding programmes 217
- Haploid induction and production in crop plants 231
- Effects of activated charcoal, cold treatment and elevated CO2-concentrations on embryogenesis in anther cultures 257
- Utilisation of anther culture in breeding Brussels sprouts 265
- The role of linkage and recombination in barley breeding with Hordeum bulbosum L. 273
- Partial incompatibility between Scandinavian six-rowed barleys and Hordeum bulbosum L. and its genetical basis 275
- Recent results on somatic embryogenesis in pea and bean 279
- Sugar beet (Beta vulgaris L.) pollen quality assessment and effect of irradiation as measured by fluorochromatic reaction and in vitro germination 283
- The detection of linkage using doubled haploids in barley 287
- The Effect Of An Elevated CO2 Concentration In Combination With Cold Treatments In Maize (Zea Mays L.) Anther Culture 291
- Obtention Of Embryos And Plants From In Vitro Culture Of Unfertilized Ovules Of Cucurbita Pepo 295
- Agronomic Value Of Androgenetic Doubled Haploid Lines As Compared To Conventionally Selected Spring Barley 299
- Pollen Plant Production In Triticum Turgidum Ssp.Durum 303
- Production Of Haploid Sugarbeets (Beta Vulgaris L.) By Ovule Culture 307
- Towards The Isolation Of Sperm Cells For Androgenic Purposes 311
- Regeneration And Selection Of Isolated Microspores Of Hordeum Vulgare 315
- Possibilities Of Obtainment And Utilization Of Doubled Haploids In Gerbera 319
- Contribution To The Studies On The First,Generative Progeny Of Kragaria X Ananassa Polyhaploids 323
- A Comparison Of Cross Prediction Methods In Spring Barley 327
- Androgenesis In Oilseed Rape 331
- Production Of Doubled Haploids In Oriental Lilies 335
- Increasing The Efficiency Of Triticale Anther Culture 339
- Genetic Gain For Some Agronomical Characters By Dihaploid Breeding In Barley 343
- Effect Of A Gametocide On The Induction Of Haploids In Triticum Aestivum 347
- The Induction Of Haploids Of Sugarbeet (Beta Vulgaris L.) Using Anther And Free Pollen Culture Or Ovule And Ovary Culture 351
- Frost Tolerant Plants Obtained From Proline Accumulating Cell Lines 355
- Somatic Cell Genetics Of Potato I. Use Of Monohaploids 359
- Factors Affecting Callus And Plant Production In Anther Cultuses Of Tomato 361
-
Part 4. In-Vitro Propagation
- Role Of Methodology In Facilitating Application Of Tissue Culture Techniques 367
- In Vitro Propagation And Breeding Of Ornamental Plants: Advantages And Disadvantages Of Variability 377
- Protoplast Culture And Use Of Regeneration Attributes To Select Somatic Hybrid Tomato Plants 405
- Sexual Reproduction In Plants By Applying The Method Of Test Tube Fertilization Of Ovules 415
- Cytogenetic Studies In Callus Cultures Of Asparagus Off 425
- Systems For Regeneration Of Cucumis Sativus Plants In Vitro 429
- Regeneration Of Temperate Fruit Trees In Vitro Via Organogenesis And Embryogenesis 433
- Problems And Prospects For The Use Of Protoplasts In Beet Breeding 437
- Shoot Redifferentiation Of Agrobacterium Transformed-Protoplasts And Plant Tissue- With Conventional Methods Not Achievable 441
- Induction Of In Vitro-Regeneration Via Somatic Embryogenesis In Pea (Pisum Sativum) And Bean (Phaseolus Vulgaris) 445
- Endogenous Cytokinins During Embryogenesis In A Carrot Cell Suspension 449
- Tissue And Protoplast Culture In Cultivated Beets 453
- Protoplast Formation In Cereals - An Assessment 457
- Exploitation For Breeding Of In Vitro Culture Of Pea Explants 461
- Some Aspects Of The In Vitro Culture Of The Beet. (Beta Vulgaris L.) 465
- Interspecific Hybridization Of Red Clover (Trifolium Pratense L.) With Alsike Clover (Trifolium Hybndum L.) Using In Vitro Embryo Rescue 469
- Pollen And Ovule Cultures Of Barley To Isolate, Manipulate And Transfer Sperm Cells In In Vitro Fertilization 473
- Strategies In High Frequency Regeneration From Diploid And Haploid Cell And Tissue Cultures Of Barley 477
- Somaclonal Variation In Plants Regenerated From Embryo Calluses In Rye (Secale Cerale L.) 479
- Somatic Embryogenesis, Cell And Protoplast Culture Of Hordeum Vulgare L. (Barley) 483
- Isolation And Culture Of Protoplasts From Callus And Suspensioncultured Cells Of Prunus Cerasus And Actinidia Chinensis 487
- Somatic Embryogenesis In Protoplast-Derived Cells Of Cucumis Melo L. 491
- A Comparative Study Of Callus Formation And Plant Regeneration From Different Explants Of Phaseolus Vulgaris And Ph. Coccineus 495
- Somatic Embryogenesis, Cell And Protoplast Culture Of Triticale (X Triticosecale Wittmack) 499
- Somatic Embryogenesis And Plant Regeneration From Meristematic Tissue Of Secale Cereale (Rye) 503
- Somatic Embryogenesis Of Triticale 507
-
Part 5. Spontaneous And Induced Variation From In-Vitro Cultures
- Variability In Tissue Culture Derived Plants - Possible Origins; Advantages And Drawbacks 513
- Screening For Virus Resistance In Tissue Culture Adventitious Regenerants And Their Progeny 535
- Chromosome Variation In Regenerated Plants 547
- In Vitro Mutagenesis In Gerbera Jamesonii 555
- In Vitro Mutagenesis In Maize 563
- Somaclones Of Wheat Regenerated From Primordial Leaf Callus 577
- Somaclonal Variation In Lotus Corniculatus L. 581
- Potential System For The Specific Selection Of Plant Mutants Overproducing Methionine 585
- Enhancement Of Asulam Resistance In Barley 589
- Triazine-Resistant Nicotiana Mutants From Photomixotrophic Cell Cultures 593
- Use Of In Vitro Culture For Inducing Variation In Rice And Fuchsia 597
- Isolation Of Auxotrophic Mutants Based On Reconstruction Experiments With Nicotiana Plumbaginifolia Protoplasts 599
- A Study On The Effect Of Different Initial Culture Media On The Chromosome Stability Of Solanum Tuberosum Cv. Maris Bard Protoplast Derived Regenerants 601
- The Effect Of Explant Source, In Vitro Regeneration And Irradiation On Variation In Yield Induced In Chrysanthemum Morifolium 607
- Transfer Of Genetic Material To Cultivated Barley From Alien Species Through Callus Culture (Preliminary Results) 611
- Somatic Embryogenesis And Plant Regeneration From Leaf Tissues Of Secale Cereale L 615
- Testing Of Salt /Nacl/ Tolerance And Regeneration In Callus Culture /n, 2n/ Of Rice 617
- The Use Of Tissue Cultures For Obtaining Tobacco Male Sterile Forms 621
- Somaclonal Variation In Triticale 625
- Separation, Identification And Biological Effects Of A Toxin Produced By Phoma Lingam 629
- Variability In Plants Of Sugar Beet (Beta Vulgaris L.) Regenerated From Callus, Cell-Suspension And Protoplasts 633
- Chromosomal Variation In Regenerated Plants From Hybrid Callus From Crosses Between Hordeum Vulgare X H. Bulbosum 637
-
Part 6. Somatic Hybridization And Cybridization
- Plant Cell Fusion As A Tool For Genetic Manipulation 641
- Genetic Improvement Of Cytoplasmic Traits Through Cytoplasmic Hybridization In Cruciferae 653
- Somatic Hybridization And Cybridization As Potential Methods For Widening Of The Gene-Pools Of Crops Within Brassicaceae And Solanaceae 663
- Investigations Into The Transfer Of Genetic Information Between Solanaceous Species And Potato By Somatic Hybridization 683
- Protoplast Culture And Fusion Of Red And Alsike Clover 685
- Interclassical Protoplast Fusion Between Orchard-Grass And Petunia 689
- Interspecific Chloroplast Recombination In A Nicotiana Somatic Hybrid 693
- Loss Of Species-Specific Sequences In Somatic Hybrids, Obtained By Fusion Of Nicotiana Tabacum Cnx Protoplasts With Heavily X-Irradiated N. Paniculata Protoplasts 697
- Somatic Cell Genetics Of Potato: Variant Cell Lines And Somatic Hybridization 701
- Somatic Hybridization Between Two Nicotiana Plumbaginifolia Lines And Between Solanum Tuberosum And Phureja Using Electrofusion 703
- Structure And Regulation Of Cytosol And Plastid Specific Isoenzymes In Higher Plants 705
- Resynthesis Of Brassica Napus Via Somatic Hybridization: A Model For Production Of Interspecific Hybrids Within Brassiceae 709
- Protoplast Culture And Plant Regeneration Of Solanum Pennellii And Lycopersicon Esculentum 713
- Somatic Cell Genetics Of Potato III: Electrofusion Of Two Amino Acid Analogue-Resistant Cell Lines 715
- Cp And Mt Genome Constitution Of Different Somatic Hybrids Between Brassica Napus And Brassica Hirta 719
-
Part 7. Isolation And Cloning Of Plant Genes
- The Complexity Of Gene Regulation During The Lightdependent Development Of Chloroplasts In Barley (Hordeum Vulgare L.) 725
- The Chloroplast Ribosomes Of Higher Plants And The Genes For Their Protein Components 731
- Genetic Variation And Gene Expression; A Study Of The Ribosomal Rna Gene Loci Of Wheat 745
- Cloning And Analysis Of Two Genes For Chalcone Synthase Of Petunia Hybrida 753
- Cm-Proteins And Thionins In Cereals: Characterization And Cloning Of Cdna 763
- Cin-Dna-Insertions Of Zea Mays 771
-
Part 8. Transformation
- Recent Progress In Plant Genetic Engineering 775
- Organ-Specific Gene Expression In Potato 777
- Electric Field Mediated Transfer Of Nucleic Acids Into Carrot Protoplasts 785
- The Pollen System Of Gene Transfer 803
- Direct Gene Transfer To Plants 813
- Genetic Manipulation In Potato 823
- Culture And Transformation Studies With Maize Protoplasts 827
- Transformation In The Forage Legumes 831
-
Part 9. Use Of Molecular Biology Methods For Plant Improvement
- Integration, Expression And Stable Transmission Through Seeds Of Foreign Genes In Plants 837
- Identification, Cloning And Transfer Of Chloroplast Genes Of Petunia Hibrida 859
- The Need For A Multidisciplinary Approach To Genetic Manipulation In Plant Breeding 867
- Appendix 883
- Author Index 895
- Subject Index 899
- Backmatter 910