1.6 General theory of electrophoresis

Chapters in this book

1. Frontmatter I
2. Preface VII
3. About the Author IX
4. Contents XI
5. Abbreviations XXIX
6. 1 Fundamentals of electrophoresis 1
7. 1.1 Electric double layer of a charged particle 5
8. 1.2 Proteins and nucleic acids form polyions in solution 13
9. 1.3 Electrophoresis is running in buffers 19
10. 1.4 Polyions are moving in electric field 27
11. 1.5 Electrophoresis is carried out in different solid media 39
12. 1.6 General theory of electrophoresis 49
13. 1.7 Electrophoresis instrumentation 55
14. 1.8 Classification of electrophoretic methods 61
15. 2 Electrophoresis of proteins 71
16. 2.1 Cellulose acetate electrophoresis of proteins 73
17. 2.2 Agarose gel electrophoresis of proteins 77
18. 2.3 Immunoelectrophoresis 109
19. 2.4 Affinity electrophoresis 119
20. 2.5 Polyacrylamide gel zone electrophoresis of proteins 131
21. 2.6 Isotachophoresis of proteins 147
22. 2.7 Disc-electrophoresis of proteins 151
23. 2.8 Isoelectric focusing of proteins 187
24. 2.9 Free-flow electrophoresis of proteins 209
25. 2.10 Capillary electrophoresis of proteins 217
26. 2.11 Two-dimensional electrophoresis 231
27. 2.12 Preparative electrophoresis of proteins 241
28. 2.13 Microchip electrophoresis of proteins 253
29. 2.14 Blotting of proteins 271
30. 2.15 Evaluation of protein pherograms 289
31. 2.16 Precast gels for protein electrophoresis. Rehydratable gels 309
32. 3 Electrophoresis of nucleic acids 317
33. 3.1 Agarose gel electrophoresis of nucleic acids 325
34. 3.2 Pulsed-field gel electrophoresis of nucleic acids 343
35. 3.3 Capillary electrophoresis of nucleic acids 349
36. 3.4 Polyacrylamide gel electrophoresis of nucleic acids 357
37. 3.5 Microchip electrophoresis of nucleic acids 371
38. 3.6 Blotting of nucleic acids 375
39. 3.7 Evaluation of nucleic acid pherograms 385
40. 3.8 Precast gels for nucleic acid electrophoresis 395
41. 4 Iontophoresis 397
42. 5 History of electrophoresis and iontophoresis 405
43. 6 Troubleshooting 421
44. Problems 435
45. Solution of problems 441
46. Reagents for electrophoresis 447
47. Recipes for electrophoresis solutions 459
48. SI units and physical constants used in electrophoresis 475
49. Electrophoresis terms 481
50. Index 485