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book: Electrophoresis Fundamentals
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Electrophoresis Fundamentals

Essential Theory and Practice
  • Budin Michov
Language: English
Published/Copyright: 2022
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About this book

The electrophoresis techniques are used in medicine, biochemistry, analytical chemistry, and biology to separate soluble and insoluble proteins, nucleic acids, chromosomes, viruses, as well as lysosomes, mitochondria, ribosomes and other cell organelles, red cells, tissue cells, and parasites. This book provides a view over the old electrophoresis techniques, as well as the recent developments in electrophoresis.

Electrophoresis Fundamentals is based on the recent book Electrophoresis: Theory and Practice published in 2020 by De Gruyter. The previous book combines theory and technical applications with troubleshooting and problem solving. While Electrophoresis is intended for specialists, Electrophoresis Fundamentals is a book for laboratory technicians, students, biochemists, general practitioners, and more.

  • Discusses modern electrophoresis methods such as the PCR technique, which is used to detect COVID-19.

  • Contains troubleshooting and problem solving.

  • Intended for students, chemists, biochemists, geneticists, physicians, and more.

Author / Editor information

Budin Michov, Sofia University, Sofia, Bulgaria.

Topics

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    Frontmatter

    I
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    Preface

    VII
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    About the Author

    IX
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    Contents

    XI
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    Abbreviations

    XXIX
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    1 Fundamentals of electrophoresis

    1
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    1.1 Electric double layer of a charged particle

    5
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    1.2 Proteins and nucleic acids form polyions in solution

    13
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    1.3 Electrophoresis is running in buffers

    19
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    1.4 Polyions are moving in electric field

    27
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    1.5 Electrophoresis is carried out in different solid media

    39
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    1.6 General theory of electrophoresis

    49
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    1.7 Electrophoresis instrumentation

    55
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    1.8 Classification of electrophoretic methods

    61
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    2 Electrophoresis of proteins

    71
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    2.1 Cellulose acetate electrophoresis of proteins

    73
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    2.2 Agarose gel electrophoresis of proteins

    77
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    2.3 Immunoelectrophoresis

    109
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    2.4 Affinity electrophoresis

    119
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    2.5 Polyacrylamide gel zone electrophoresis of proteins

    131
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    2.6 Isotachophoresis of proteins

    147
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    2.7 Disc-electrophoresis of proteins

    151
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    2.8 Isoelectric focusing of proteins

    187
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    2.9 Free-flow electrophoresis of proteins

    209
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    2.10 Capillary electrophoresis of proteins

    217
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    2.11 Two-dimensional electrophoresis

    231
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    2.12 Preparative electrophoresis of proteins

    241
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    2.13 Microchip electrophoresis of proteins

    253
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    2.14 Blotting of proteins

    271
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    2.15 Evaluation of protein pherograms

    289
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    2.16 Precast gels for protein electrophoresis. Rehydratable gels

    309
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    3 Electrophoresis of nucleic acids

    317
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    3.1 Agarose gel electrophoresis of nucleic acids

    325
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    3.2 Pulsed-field gel electrophoresis of nucleic acids

    343
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    3.3 Capillary electrophoresis of nucleic acids

    349
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    3.4 Polyacrylamide gel electrophoresis of nucleic acids

    357
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    3.5 Microchip electrophoresis of nucleic acids

    371
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    3.6 Blotting of nucleic acids

    375
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    3.7 Evaluation of nucleic acid pherograms

    385
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    3.8 Precast gels for nucleic acid electrophoresis

    395
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    4 Iontophoresis

    397
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    5 History of electrophoresis and iontophoresis

    405
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    6 Troubleshooting

    421
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    Problems

    435
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    Solution of problems

    441
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    Reagents for electrophoresis

    447
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    Recipes for electrophoresis solutions

    459
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    SI units and physical constants used in electrophoresis

    475
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    Electrophoresis terms

    481
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    Index

    485
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Publishing information
Pages and Images/Illustrations in book
eBook published on:
April 4, 2022
eBook ISBN:
9783110761641
Paperback published on:
April 18, 2022
Paperback ISBN:
9783110761627
Pages and Images/Illustrations in book
Front matter:
31
Main content:
490
Illustrations:
12
Coloured Illustrations:
149
Tables:
50
https://doi.org/10.1515/9783110761641
eBook ISBN: 9783110761641
Paperback ISBN: 9783110761627
Keywords for this book
Electrophoresis; Biochemistry; Chemistry; Analytical Chemistry; Laboratory Medicine.
Audience(s) for this book
Graduate students in chemistry, biochemistry, molecular biology and laboratory medicine.
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