15 In vivo multiphoton microscopy of human skin

Kapitel in diesem Buch

1. Frontmatter i
2. Preface vii
3. Foreword xi
4. Contents xiii
5. List of contributing authors xxiii
6. Part I: Basics
7. 1 Brief history of fluorescence lifetime imaging 3
8. 2 The long journey to the laser and its use for nonlinear optics 17
9. 3 Advanced TCSPC-FLIM techniques 23
10. 4 Ultrafast lasers in biophotonics 53
11. Part II: Modern nonlinear microscopy of live cells
12. 5 STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities 85
13. 6 Principles and applications of temporal-focusing wide-field two-photon microscopy 103
14. 7 FLIM-FRET microscopy 141
15. 8 TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing 163
16. 9 Laser tweezers are sources of two-photon effects 177
17. 10 Metabolic shifts in cell proliferation and differentiation 189
18. 11 Femtosecond laser nanoprocessing 209
19. 12 Cryomultiphoton imaging 227
20. Part III: Nonlinear tissue imaging
21. 13 Multiphoton Tomography (MPT) 247
22. 14 Clinical multimodal CARS imaging 269
23. 15 In vivo multiphoton microscopy of human skin 287
24. 16 Two-photon microscopy and fluorescence lifetime imaging of the cornea 301
25. 17 Multiscale correlative imaging of the brain 321
26. 18 Revealing interaction of dyes and nanomaterials by multiphoton imaging 345
27. 19 Multiphoton FLIM in cosmetic clinical research 369
28. 20 Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery 395
29. 21 Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models 411
30. 22 Bedside assessment of multiphoton tomography 425
31. Index 445