Effect of animal age and gender on fatty acid and elemental composition in Austrian beef applicable for authentication purposes
Abstract
The main aim of the present study was to find differences in the content of fatty acids and variations in elemental composition in beef samples of longissimus dorsi muscle related to cattle age and gender. A further goal was to describe interrelations among the selected variables (descriptors) characterising the samples. For this purpose, an extensive data table was compiled, which contains chemical descriptors specifying forty-six beef samples originating from four well-known Austrian grassland-based beef labels. The following descriptors were investigated: (a) concentrations of 33 fatty acids, (b) concentrations of 19 elements, (c) contents of dry-mass, protein, intramuscular fat, and ash, (d) total content of saturated fatty acids (SFA), mono-unsaturated fatty acids (MUFA), and poly-unsaturated fatty acids (PUFA), (e) total contents of omega-3 (n-3) and omega-6 (n-6) PUFA and their ratio. The correlation analysis provided a number of statistically significant correlations among the descriptors, which were concordant with the results of the principal component analysis and cluster analysis. Furthermore, the effect of age and gender of cattle (both acting as target factors) on the fatty acid content and elemental composition of beef was examined by analysis of variance (ANOVA) and appropriate non-parametric tests. Several important interrelations among the beef characteristics investigated were also discovered. Finally, the most relevant beef descriptors were utilised in linear discrimination analysis (LDA) for predicting the slaughter age of the cattle for beef authentication.
[1] Alfaia, C. P. M., Alves, S. P., Martins, S. I. V., Costa, A. S. H., Fontes, C. M. G. A., Lemos, J. P. C., Bessa, R. J. B., & Prates, J. A. M. (2009). Effect of the feeding system on intramuscular fatty acids and conjugated linoleic acid isomers of beef cattle, with emphasis on their nutritional value and discriminatory ability. Food Chemistry, 114, 939–946. DOI: 10.1016/j.foodchem.2008.10.041. http://dx.doi.org/10.1016/j.foodchem.2008.10.04110.1016/j.foodchem.2008.10.041Search in Google Scholar
[2] Ballin, N. Z. (2010). Authentication of meat and meat products. Meat Science, 86, 577–587. DOI: 10.1016/j.meatsci.2010.06.001. http://dx.doi.org/10.1016/j.meatsci.2010.06.00110.1016/j.meatsci.2010.06.001Search in Google Scholar
[3] Banskalieva, V., Sahlu, T., & Goetsch, A. L. (2000). Fatty acid composition of goat muscles and fat depots: a review. Small Ruminant Research, 37, 255–268. DOI: 10.1016/s0921-4488(00)00128-0. http://dx.doi.org/10.1016/S0921-4488(00)00128-010.1016/S0921-4488(00)00128-0Search in Google Scholar
[4] Bhattacharya, A., Banu, J., Rahman, M., Causey, J., & Fernandes, G. (2006). Biological effects of conjugated linoleic acids in health and disease. The Journal of Nutritional Biochemistry, 17, 789–810. DOI: 10.1016/j.jnutbio.2006.02.009. http://dx.doi.org/10.1016/j.jnutbio.2006.02.00910.1016/j.jnutbio.2006.02.009Search in Google Scholar PubMed
[5] Brereton, R. G. (2003). Chemometrics: Data analysis for the laboratory and chemical plant. Chichester, UK: Wiley. 10.1002/0470863242Search in Google Scholar
[6] De Smet, S., Raes, K., & Demeyer, D. (2004). Meat fatty acid composition as affected by fatness and genetic factors: a review. Animal Research, 53, 81–98. DOI: 10.1051/animres:2004003. http://dx.doi.org/10.1051/animres:200400310.1051/animres:2004003Search in Google Scholar
[7] Ertl, K. (2009). Quality of Austrian beef with respect to its elemental composition. In Proceedings of YISAC 2009, June 29–July 2, 2009 (pp. 60). Graz, Austria: Karl-Franzens University. Search in Google Scholar
[8] Harris, W. S., Miller, M., Tighe, A. P., Davidson, M. H., & Schaefer, E. J. (2008). Omega-3 fatty acids and coronary heart disease risk: Clinical and mechanistic perspectives. Atherosclerosis, 197, 12–24. DOI: 10.1016/j.atherosclerosis.2007.11.008. http://dx.doi.org/10.1016/j.atherosclerosis.2007.11.00810.1016/j.atherosclerosis.2007.11.008Search in Google Scholar PubMed
[9] Jutzeler van Wijlen, R. P., & Colombani, P. C. (2010). Grassbased ruminant production methods and human bioconversion of vaccenic acid with estimations of maximal dietary intake of conjugated linoleic acids. International Dairy Journal, 20, 433–448. DOI: 10.1016/j.idairyj.2010.01.008. http://dx.doi.org/10.1016/j.idairyj.2010.01.00810.1016/j.idairyj.2010.01.008Search in Google Scholar
[10] Lourenço, M., Van Ranst, G., Vlaeminck, B., De Smet, S., & Fievez, V. (2008). Influence of different dietary forages on the fatty acid composition of rumen digesta as well as ruminant meat and milk. Animal Feed Science and Technology, 145, 418–437. DOI: 10.1016/j.anifeedsci.2007.05.043. http://dx.doi.org/10.1016/j.anifeedsci.2007.05.04310.1016/j.anifeedsci.2007.05.043Search in Google Scholar
[11] Malau-Aduli, A. E., Siebert, B. D., Bottema, C. D., & Pitchford, W. S. (1998). Breed comparison of the fatty acid composition of muscle phospholipids in Jersey and Limousin cattle. Journal of Animal Science, 76, 766–773. 10.2527/1998.763766xSearch in Google Scholar PubMed
[12] Massart, D. L., Vandeginste, B. G. M., Deming, S. N., Michotte, Y., & Kaufman, L. (1997). Handbook of chemometrics and qualimetrics: Part A. Amsterdam, The Netherlands: Elsevier. Search in Google Scholar
[13] Moloney, A. P., Scollan, N. D., & Miles, L. (2008). Enrichment of n-3 fatty acids and conjugated linoleic acid in beef: ProSafeBeef. Nutrition Bulletin, 33, 374–381. DOI: 10.1111/j.1467-3010.2008.00737.x. http://dx.doi.org/10.1111/j.1467-3010.2008.00737.x10.1111/j.1467-3010.2008.00737.xSearch in Google Scholar
[14] Moreno, J. J., & Mitjavila, M. T. (2003). The degree of unsaturation of dietary fatty acids and the development of atherosclerosis (review). The Journal of Nutritional Biochemistry, 14, 182–195. DOI: 10.1016/s0955-2863(02)00294-2. http://dx.doi.org/10.1016/S0955-2863(02)00294-210.1016/S0955-2863(02)00294-2Search in Google Scholar
[15] Mosley, E. E., Shafii, B., Moate, P. J., & McGuire, M. A. (2006). cis-9, trans-11 Conjugated linoleic acid is synthesized directly from vaccenic acid in lactating dairy cattle. Journal of Nutrition, 136, 570–575. 10.1093/jn/136.3.570Search in Google Scholar
[16] Pariza, M. W., Park, Y. H., & Cook, M. E. (2001). The biologically active isomers of conjugated linoleic acid. Progress in Lipid Research, 40, 283–298. DOI: 10.1016/s0163-7827(01)00008-x. http://dx.doi.org/10.1016/S0163-7827(01)00008-X10.1016/S0163-7827(01)00008-XSearch in Google Scholar
[17] Park, Y. H. (2009). Conjugated linoleic acid (CLA): Good or bad trans fat? Journal of Food Composition and Analysis, 22, S4–S12. DOI: 10.1016/j.jfca.2008.12.002. http://dx.doi.org/10.1016/j.jfca.2008.12.00210.1016/j.jfca.2008.12.002Search in Google Scholar
[18] Purchas, R. W., Knight, T. W., & Busboom, J. R. (2003). The effect of production system and age on concentration of fatty acids in intramuscular fat of the longissimus and triceps brachii muscles of Angus-cross heifers. Meat Science, 70, 597–603. DOI: 10.1016/j.meatsci.2004.12.020. http://dx.doi.org/10.1016/j.meatsci.2004.12.02010.1016/j.meatsci.2004.12.020Search in Google Scholar PubMed
[19] Raes, K., De Smet, S., & Demeyer, D. (2004). Effect of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meet: a review. Animal Feed Science and Technology, 113, 199–221. DOI: 10.1016/j.anifeedsci.2003.09.001. http://dx.doi.org/10.1016/j.anifeedsci.2003.09.00110.1016/j.anifeedsci.2003.09.001Search in Google Scholar
[20] Ruxton, C. H. S., Reed, S. C., Simpson, M. J. A., & Millington, K. J. (2004). The health benefits of omega-3 polyunsaturated fatty acids: a review of the evidence. Journal of Human Nutrition and Dietetics, 17, 449–459. DOI: 10.1111/j.1365-277x.2004.00552.x. http://dx.doi.org/10.1111/j.1365-277X.2004.00552.x10.1111/j.1365-277X.2004.00552.xSearch in Google Scholar PubMed
[21] Scollan, N., Hocquette, J. F., Nuernberg, K., Dannenberger, D., Richardson, I., & Moloney, A. (2006). Innovations in beef production systems that enhance the nutritional and health value of beef lipids and their relationship with meat quality. Meat Science, 74, 17–33. DOI: 10.1016/j.meatsci.2006.05.002. http://dx.doi.org/10.1016/j.meatsci.2006.05.00210.1016/j.meatsci.2006.05.002Search in Google Scholar PubMed
[22] Schönfeldt, H. C., Naudé, R. T., & Boshoff, E. (2010). Effect of age and cut on the nutritional content of South African beef. Meat Science, 86, 674–683. DOI: 10.1016/j.meatsci.2010.06.004. http://dx.doi.org/10.1016/j.meatsci.2010.06.00410.1016/j.meatsci.2010.06.004Search in Google Scholar PubMed
[23] Simopoulos, A. P. (2004). omega-6/omega-3 Essential fatty acid ratio and chronic diseases. Food Reviews International, 20, 77–90. DOI: 10.1081/fri-120028831. http://dx.doi.org/10.1081/FRI-12002883110.1081/FRI-120028831Search in Google Scholar
[24] Varmuza, K., & Filzmoser, P. (2009). Introduction to multivariate statistical analysis in chemometrics. Boca Raton, FL, USA: CRC Press. http://dx.doi.org/10.1201/978142005949610.1201/9781420059496Search in Google Scholar
[25] Velik, M., Eingang, D., Kaufmann, J., & Kitzer, R. (2009). Fleischqualität Oesterreichischer Rindfleisch-Markenprogramme (Ochse, Kalbin, Jungrind) — Ergebnisse einer Stichprobenerhebung. In Proceedings of the 36. Viehwirtschaftliche Fachtagung, April 16–17, 2009 (pp. 85–93). Irdning: LFZ Raumberg-Gumpenstein. Search in Google Scholar
[26] Webb, E. C., & O’Neill, H. A. (2008). The animal fat paradox and meat quality. Meat Science, 80, 28–36. DOI: 10.1016/j.meatsci.2008.05.029. http://dx.doi.org/10.1016/j.meatsci.2008.05.02910.1016/j.meatsci.2008.05.029Search in Google Scholar PubMed
[27] Woods, V. B., & Fearon, A. M. (2009). Dietary sources of unsaturated fatty acids for animals and their transfer into meat, milk and eggs: A review. Livestock Science, 126, 1–20. DOI: 10.1016/j.livsci.2009.07.002. http://dx.doi.org/10.1016/j.livsci.2009.07.00210.1016/j.livsci.2009.07.002Search in Google Scholar
© 2012 Institute of Chemistry, Slovak Academy of Sciences
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Articles in the same Issue
- Content of selected secondary metabolites in wild hop
- Continuous sorption of synthetic dyes on dried biomass of microalga Chlorella pyrenoidosa
- Sludge of wastewater treatment plants as Co2+ ions sorbent
- Effect of animal age and gender on fatty acid and elemental composition in Austrian beef applicable for authentication purposes
- Nutritional, antioxidant, and glycaemic characteristics of new functional bread
- Effects of enzymes and hydrocolloids on physical, sensory, and shelf-life properties of wheat bread
- Magnetic chains formed from tetra-coordinate Co(II) complexes
- Prediction of anti-tuberculosis activity of 3-phenyl-2H-1,3-benzoxazine-2,4(3H)-dione derivatives
- Experimental investigation of bubble and drop formation at submerged orifices
- Cadmium concentration stabilization in a continuous sulfate reducing bioreactor via sulfide concentration control
- Facile synthesis of gemini surface-active ATRP initiator and its use in soap-free AGET ATRP mini-emulsion polymerisation
- Bulgarian natural diatomites: modification and characterization
- Synthesis, characterisation, and DC conductivity of polyaniline-lead oxide composites