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Aquaculture by-product: a source of proteolytic enzymes for detergent additives

  • Chirleanny Mendes EMAIL logo , Marília Brito , Tatiana Porto , Ana Porto , Ranilson Bezerra , Luiz Carvalho , Ana Caneiro-Leão and Maria Carneiro-da-Cunha
Published/Copyright: October 8, 2009
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Chemical Papers
From the journal Chemical Papers Volume 63 Issue 6

Abstract

Intestine proteases of Nile tilapia (Oreochromis niloticus) were partially purified by heat treatment (purification factor of 3.5, enzyme activity remained almost constant) to reach the maximum activity and stability within an alkaline pH range of 7.2–11.0. The optimum temperature and stability over a 120 min period were found to be at 55°C and at 35–45°C, respectively. The proteases’ activity was not affected by a 1 vol. % saponin surfactant, inactivated by 0.01 g mL−1 sodium dodecylsulphate after 120 min, and it remained stable for 30 min in a 5 vol. % and 10 vol. % hydrogen peroxide solutions. The proteases were slightly activated by Ca2+, Mg2+, and K+ and the substrate most effectively hydrolysed was casein (40.0 U mg−1). A 24 full factorial design used to evaluated the influence of independent variables showed that the enzyme extract, detergent concentration and the incubation time had a significant influence on the enzymatic activity. The best conditions to be used concerning detergent additive were found with 0.3 mg mL−1 of protein and 3.0 mg mL−1 of detergent for 30 min in the presence of Astrus® detergent.

Keywords: protease; Nile tilapia; laundry detergent; surfactant; oxidising agents

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Published Online: 2009-10-8
Published in Print: 2009-12-1

© 2009 Institute of Chemistry, Slovak Academy of Sciences

Articles in the same Issue

  1. Utilization of solid phase spectrophotometry for the determination of trace amounts of copper using 5-(2-benzothiazolylazo)-8-hydroxyquinoline
  2. Analysis of spectinomycin in fermentation broth by reversed-phase chromatography
  3. An amperometric sensor for uric acid based on ordered mesoporous carbon-modified pyrolytic graphite electrode
  4. Utility of π-acceptor reagents for spectrophotometric determination of sulphonamide drugs via charge-transfer complex formation
  5. A graph theoretical approach to the effect of mutation on the flexibility of the DNA binding domain of p53 protein
  6. Aquaculture by-product: a source of proteolytic enzymes for detergent additives
  7. Effect of anthraquinone on brightness value and crystalline structure of pulp during soda processes
  8. Selection of a method for determination of activity of pectinolytic enzymes in berry fruit materials
  9. Study on polymeric micelles of poly(γ-benzyl l-glutamate)-graft-poly(ethylene glycol) copolymer and its mixtures with poly(γ-benzyl l-glutamate) homopolymer in ethanol
  10. Synthesis and characterization of mesoporous molecular sieves
  11. Growth mechanism and characterization of ZnO nano-tubes synthesized using the hydrothermal-etching method
  12. Novel use of silicon nanocrystals and nanodiamonds in biology
  13. Fluoride anion sensing using colorimetric reagents containing binaphthyl moiety and urea binding site
  14. Spectrophotometric methods for sertraline hydrochloride and/or clidinium bromide determination in bulk and pharmaceutical preparations
  15. Study of physicochemical properties-antitubercular activity relationship of naphthalene-1,4-dione analogs: A QSAR approach
  16. Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine
  17. Rheological properties of doughs with buckwheat and quinoa additives
  18. Synthesis and isolation of methyl bismuth cysteine and its definitive identification by high resolution mass spectrometry
https://doi.org/10.2478/s11696-009-0081-z
Keywords for this article
protease; Nile tilapia; laundry detergent; surfactant; oxidising agents

Articles in the same Issue

  1. Utilization of solid phase spectrophotometry for the determination of trace amounts of copper using 5-(2-benzothiazolylazo)-8-hydroxyquinoline
  2. Analysis of spectinomycin in fermentation broth by reversed-phase chromatography
  3. An amperometric sensor for uric acid based on ordered mesoporous carbon-modified pyrolytic graphite electrode
  4. Utility of π-acceptor reagents for spectrophotometric determination of sulphonamide drugs via charge-transfer complex formation
  5. A graph theoretical approach to the effect of mutation on the flexibility of the DNA binding domain of p53 protein
  6. Aquaculture by-product: a source of proteolytic enzymes for detergent additives
  7. Effect of anthraquinone on brightness value and crystalline structure of pulp during soda processes
  8. Selection of a method for determination of activity of pectinolytic enzymes in berry fruit materials
  9. Study on polymeric micelles of poly(γ-benzyl l-glutamate)-graft-poly(ethylene glycol) copolymer and its mixtures with poly(γ-benzyl l-glutamate) homopolymer in ethanol
  10. Synthesis and characterization of mesoporous molecular sieves
  11. Growth mechanism and characterization of ZnO nano-tubes synthesized using the hydrothermal-etching method
  12. Novel use of silicon nanocrystals and nanodiamonds in biology
  13. Fluoride anion sensing using colorimetric reagents containing binaphthyl moiety and urea binding site
  14. Spectrophotometric methods for sertraline hydrochloride and/or clidinium bromide determination in bulk and pharmaceutical preparations
  15. Study of physicochemical properties-antitubercular activity relationship of naphthalene-1,4-dione analogs: A QSAR approach
  16. Spectroscopic study of protonation of oligonucleotides containing adenine and cytosine
  17. Rheological properties of doughs with buckwheat and quinoa additives
  18. Synthesis and isolation of methyl bismuth cysteine and its definitive identification by high resolution mass spectrometry
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