Improved fluorimetric measurement of uranium uptake and distribution in spring wheat (Triticum aestivum L.)
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Cătălin Borcia
Abstract
Uranium uptake and (radio)toxicity was tested on spring wheat (Triticum aestivum L.) in a laboratory study using differently concentrated uranium nitrate solutions. Within these experiments, two analytical assays of uranium were comparatively tested: a fast and improved fluorimetric assay and the classical colorimetric (U(IV)–arsenazo(III) complexation) one. During the germination, the wheat seeds and plantlets supported well the uranium solutions of treatment within the entire concentration range (1E-4–5E-3 M). Uranium proved to be non (radio)toxic to wheat as compared with other natural and anthropogenic radiocations, probably because its uptake by spring wheat during the germination is low. Indeed, only a small fraction of uranium administered was located within the roots, whereas the uranium content of the stems was negligible. A high correlation between the results obtained by two analytical methods was found. However, the fluorimetric assay proved to be more reliable and fast, and accurate.
Acknowledgement
This work was supported by the strategic grant POSDRU/159/1.5/S/133652, co-financed by the European Social Fund within the Sectorial Operational Program Human Resources Development 2007–2013.
©2016 Walter de Gruyter Berlin/Boston
Articles in the same Issue
- Frontmatter
- Formation cross-sections and chromatographic separation of protactinium isotopes formed in proton-irradiated thorium metal
- Experimental determination of proton induced reaction cross sections on natNi near threshold energy
- Improved fluorimetric measurement of uranium uptake and distribution in spring wheat (Triticum aestivum L.)
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Articles in the same Issue
- Frontmatter
- Formation cross-sections and chromatographic separation of protactinium isotopes formed in proton-irradiated thorium metal
- Experimental determination of proton induced reaction cross sections on natNi near threshold energy
- Improved fluorimetric measurement of uranium uptake and distribution in spring wheat (Triticum aestivum L.)
- Radioactive 210Po in magnesium supplements
- Preclinical dosimetric estimation of [111In] 5, 10, 15, 20-tetra phenyl porphyrin complex as a possible imaging/PDT agent
- Development of 177Lu-DTPA-SPIO conjugates for potential use as a dual contrast SPECT/MRI imaging agent
- Radioiodination and biological evaluation of levalbuterol as a new selective radiotracer: a β2-adrenoceptor agonist
