Development of 177Lu-DTPA-SPIO conjugates for potential use as a dual contrast SPECT/MRI imaging agent
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Saeed Shanehsazzadeh
,
Cordula Grüttner
Abstract
This study describes the preparation, biodistribution of 177Lu-DTPA-SPIO after intravenous injection in rats. The chelator DTPA dianhydride was conjugated to SPIO NPs using a small modification of the well-known cyclic anhydride method. Conjugation was done at a 1:2 (SPIO : ccDTPA) molar ratio. Conjugation reaction was purified with Magnetic assorting column (MACs) using high gradient magnetic field following incubation, the radio labeled conjugate was checked using RTLC method for labeling and purity checked. The RTLC showed that labeling yield was above 99% after purification and the compound have good in-vitro stabilities until 48 h post injection in the presence of human serum. The biodistribution of 177Lu-DTPA-SPIO in rats showed dramatic uptake in the reticuloendothelial system (RES) and their clearance is so fast in other organs especially in the blood. In conclusion, due to high uptakes of this radiotracer in the liver and spleen and their fast clearance from other tissues, especially in blood, it is suggested that this radiotracer would be suitable for RES studies.
Acknowledgement
We acknowledge the financial support of the International Atomic Energy Organization under “CRP F22064”.
©2016 Walter de Gruyter Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Formation cross-sections and chromatographic separation of protactinium isotopes formed in proton-irradiated thorium metal
- Experimental determination of proton induced reaction cross sections on natNi near threshold energy
- Improved fluorimetric measurement of uranium uptake and distribution in spring wheat (Triticum aestivum L.)
- Radioactive 210Po in magnesium supplements
- Preclinical dosimetric estimation of [111In] 5, 10, 15, 20-tetra phenyl porphyrin complex as a possible imaging/PDT agent
- Development of 177Lu-DTPA-SPIO conjugates for potential use as a dual contrast SPECT/MRI imaging agent
- Radioiodination and biological evaluation of levalbuterol as a new selective radiotracer: a β2-adrenoceptor agonist
