Experimental neonatal hypoxia ischemia causes long lasting changes of oxidative stress parameters in the hippocampus and the spleen
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Felipe Kawa Odorcyk
, Janaína Kolling
, Eduardo Farias Sanches , Angela T.S. Wyse and Carlos Alexandre Netto
Abstract
Neonatal hypoxia ischemia (HI) is the main cause of mortality and morbidity in newborns. The mechanisms involved in its progression start immediately and persist for several days. Oxidative stress and inflammation are determinant factors of the severity of the final lesion. The spleen plays a major part in the inflammatory response to HI. This study assessed the temporal progression of HI-induced alterations in oxidative stress parameters in the hippocampus, the most affected brain structure, and in the spleen. HI was induced in Wistar rat pups in post-natal day 7. Production of reactive oxygen species (ROS), and the activity of the anti oxidant enzyme superoxide dismutase and catalase were assessed 24 h, 96 h and 38 days post-HI. Interestingly, both structures showed a similar pattern, with few alterations in the production of ROS species up to 96 h often combined with an increased activity of the anti oxidant enzymes. However, 38 days after the injury, ROS were at the highest in both structures, coupled with a decrease in the activity of the enzymes. Altogether, present results suggest that HI causes long lasting alterations in the hippocampus as well as in the spleen, suggesting a possible target for delayed treatments for HI.
Acknowledgments
This study was partly supported by financial resources from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Author’s statement
Conflict of interest: Authors state no conflict of interest.
Material and methods: Informed consent: Informed consent has been obtained from all individuals included in this study.
Ethical approval: The research related to human subject use has complied with all the relevant national regulations, and institutional policies, and is in accordance with the tenets of the Helsinki Declaration, and has been approved by the authors’ institutional review board or equivalent committee.
References
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Articles in the same Issue
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- Corner of Academy
- Pregnancy after malignant disease – challenges and possibilities
- Review articles
- Animatio: a history of ideas on the beginning of personhood
- Expectant management of caesarean scar ectopic pregnancy: a systematic review
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- Comparison of healthcare utilization and outcomes by gestational diabetes diagnostic criteria
- Adverse pregnancy outcomes and inherited thrombophilia
- Effect of female genital cutting performed by health care professionals on labor complications in Egyptian women: a prospective cohort study
- Letters to the Editor
- Effect of female genital cutting performed by health care professionals on labor complications in Egyptian women: methodological concerns
- Reply to: Effect of female genital cutting performed by health care professionals on labor complications in Egyptian women: methodological concerns
- Short communication
- Maternal plasma LPCAT 1 mRNA correlates with lamellar body count
- Original articles
- Experimental neonatal hypoxia ischemia causes long lasting changes of oxidative stress parameters in the hippocampus and the spleen
- Altered thymocyte and T cell development in neonatal mice with hyperoxia-induced lung injury
Articles in the same Issue
- Frontmatter
- Editorial
- Maternal medicine: an evolving discipline
- Corner of Academy
- Pregnancy after malignant disease – challenges and possibilities
- Review articles
- Animatio: a history of ideas on the beginning of personhood
- Expectant management of caesarean scar ectopic pregnancy: a systematic review
- Highlight articles
- Maternal outcomes in first and second trimester termination of pregnancy: which are the risk factors?
- A preliminary study of uterine scar tissue following cesarean section
- Catastrophic antiphospholipid syndrome (Ronald Asherson syndrome) and obstetric pathology
- Comparison of healthcare utilization and outcomes by gestational diabetes diagnostic criteria
- Adverse pregnancy outcomes and inherited thrombophilia
- Effect of female genital cutting performed by health care professionals on labor complications in Egyptian women: a prospective cohort study
- Letters to the Editor
- Effect of female genital cutting performed by health care professionals on labor complications in Egyptian women: methodological concerns
- Reply to: Effect of female genital cutting performed by health care professionals on labor complications in Egyptian women: methodological concerns
- Short communication
- Maternal plasma LPCAT 1 mRNA correlates with lamellar body count
- Original articles
- Experimental neonatal hypoxia ischemia causes long lasting changes of oxidative stress parameters in the hippocampus and the spleen
- Altered thymocyte and T cell development in neonatal mice with hyperoxia-induced lung injury