Abstract
The insulin-like growth factors and their binding proteins are important for placental and foetal growth. In this study, we have investigated the presence of proteolytic activity directed against insulin-like growth factor binding protein-1 (IGFBP-1) in pregnancy. In addition, the effect of protease activity on IGFBP-1 immunoreactivity and IGF binding was characterised. 125I-IGFBP-1 was incubated with maternal and foetal serum, amniotic fluid and placental extracts. Breakdown of 125I-IGFBP-1 was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and autoradiography. The size distribution of endogenous IGFBP-1 was determined by Western immunoblotting. Protease inhibitor studies characterised the proteolytic activity, and Western ligand blotting with 125I-IGF-I was used to determine IGF binding capacity of proteolysed IGFBP-1. Amniotic fluid samples collected after labour onset contained proteolytic activity that generated 12- and 19-kDa IGFBP-1 fragments that did not bind to 125I-IGF-I. This activity was not detected in amniotic fluid collected prior to labour onset or in other tissues. Activity was blocked by aprotinin, leupeptin, phenyl methyl sulphonyl fluoride, and Kunitz soybean trypsin inhibitor but not by ethylene diamine tetraacetic acid or pepstatin. Incubation of IGFBP-1 with trypsin generated fragments of a similar size to the amniotic fluid protease. In conclusion, we have demonstrated the presence in vivo of a trypsin-like proteolytic activity that alters the IGF-binding function of IGFBP-1 in pregnancy.
The authors stated that there are no conflicts of interest regarding the publication of this article.
We are grateful to SPARKS for financial support of this project.
References
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©2013 by Walter de Gruyter Berlin Boston
Articles in the same Issue
- Masthead
- Masthead
- Review
- The diagnosis of rupture of fetal membranes (ROM): a meta-analysis
- Original Articles – Obstetrics
- Differential proteolysis of insulin-like growth factor binding protein-1 (IGFBP-1) in pregnancy
- Hyperoxic resuscitation after hypoxia-ischemia induces cerebral inflammation that is attenuated by tempol in a reporter mouse model with very young mice
- Prenatal maternal stress predicts cord-blood ferritin concentration
- Puerperal curettage after cesarean section delivery
- Psychological reactions related to fetal magnetic resonance imaging: a follow-up study
- Ethnic disparity in amniotic fluid levels of hyaluronan, histone H2B and superoxide dismutase in spontaneous preterm birth
- Mode of delivery in a subsequent pregnancy following previous instrumental delivery
- Original Articles – Fetus
- Pregnancy and neonatal outcome following an antenatal diagnosis of cleft lip and palate
- Genetic low nephron number hypertension is associated with altered expression of osteopontin and CD44 during nephrogenesis*
- Pharmacovigilance in pregnancy: adverse drug reactions associated with fetal disorders
- Expectant management in type II selective intrauterine growth restriction and abnormal chord insertion in monochorionic twins
- Can prenatal detection of Down syndrome be improved by enhancing obstetricians’ skills of performing adequate foetal cardiac examination at the primary level?a
- Original Articles – Newborn
- The national perinatal mortality rate in the State of Qatar during 2011; trends since 1990 and comparative analysis with selected high-income countries: The PEARL Study Project*
- Determinants of successful breastfeeding initiation in healthy term singletons: a Swiss university hospital observational study
- Congress Calendar
- Congress Calendar
Articles in the same Issue
- Masthead
- Masthead
- Review
- The diagnosis of rupture of fetal membranes (ROM): a meta-analysis
- Original Articles – Obstetrics
- Differential proteolysis of insulin-like growth factor binding protein-1 (IGFBP-1) in pregnancy
- Hyperoxic resuscitation after hypoxia-ischemia induces cerebral inflammation that is attenuated by tempol in a reporter mouse model with very young mice
- Prenatal maternal stress predicts cord-blood ferritin concentration
- Puerperal curettage after cesarean section delivery
- Psychological reactions related to fetal magnetic resonance imaging: a follow-up study
- Ethnic disparity in amniotic fluid levels of hyaluronan, histone H2B and superoxide dismutase in spontaneous preterm birth
- Mode of delivery in a subsequent pregnancy following previous instrumental delivery
- Original Articles – Fetus
- Pregnancy and neonatal outcome following an antenatal diagnosis of cleft lip and palate
- Genetic low nephron number hypertension is associated with altered expression of osteopontin and CD44 during nephrogenesis*
- Pharmacovigilance in pregnancy: adverse drug reactions associated with fetal disorders
- Expectant management in type II selective intrauterine growth restriction and abnormal chord insertion in monochorionic twins
- Can prenatal detection of Down syndrome be improved by enhancing obstetricians’ skills of performing adequate foetal cardiac examination at the primary level?a
- Original Articles – Newborn
- The national perinatal mortality rate in the State of Qatar during 2011; trends since 1990 and comparative analysis with selected high-income countries: The PEARL Study Project*
- Determinants of successful breastfeeding initiation in healthy term singletons: a Swiss university hospital observational study
- Congress Calendar
- Congress Calendar