Cardioprotective effect of Justicia gendarussa on doxorubicin induced toxicity in mice
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Sreepriya P. K.
Abstract
Justicia gendarussa Burm.f, belonging to the family Acanthaceae, is widely used for various ailments traditionally. Antioxidant, anti-arthritic, anti-inflammatory, analgesic, anticancerous, properties of the plant have been widely reported. The present study analyzed the cardioprotective effect of J. gendarussa on doxorubicin (DOX) induced toxicity in mice. Ethanolic extract of J. gendarussa was administered orally for 7 consecutive days. The alterations in oxido-reduction status, biochemical and histopathological parameters were analyzed in heart tissue. DOX increased superoxide dismutase (SOD) and catalase activities to 3.4 ± 0.5 and 3.68 ± 1 from their normal values 2.43 ± 0.8 and 2.72 ± 0.88, respectively. The increased activities of both the enzymes were found reduced to 3.12 ± 0.24 and 3.41 ± 0.65 by the treatment of the extract. Similarly, DOX elevated glutathione peroxidase (GPx) activity to 44.6 ± 3.71 from the normal level 32.33 ± 3.41. DOX decreased the glutathione (GSH) level to 15.66 ± 2.51 from the normal values 31.66 ± 4.05. Upon treatment, GPx activity and GHS level found restored. The increased lipid peroxidation 2.53 ± 0.25 of DOX was also decreased to 2.0 ± 0.34 by the extract. Histopathology observations substantiate the protective effect of J. gendarussa extract. In conclusion, DOX-induced disturbance of oxido-reduction status and histopathology of heart attenuated closer to the normal indicating the protective effect of J. gendarussa against DOX-induced toxicity in cardiomyocytes.
Acknowledgments
The authors are grateful to Amala Cancer Research Centre, Thrissur, Kerala, for providing all facilities and support for conducting this project work.
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Research funding: The authors are thankful to Amala Cancer Research Centre for providing the research fellowship to undertake this work.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Informed consent was obtained from all individuals included in this study.
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Ethical approval: The animal experiments accomplished in this study were carried out with the prior approval of the Institutional Animal Ethics Committee (IAEC) and conducted strictly according to the guidelines of Committee for the purpose of Control and Supervision of Experiments on Animals (CPCSEA) constituted by the Animal Welfare Division, Government of India.
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Declaration of interest: The authors report no conflicts of interest.
References
1. Ratnasooriya, W, Deraniyagala, S, Dehigaspitiya, D. Antinociceptive activity and toxicological study of aqueous leaf extract of Justicia gendarussa Burm. F. in rats. J Pharmacogn Mag 2007;3:145.Search in Google Scholar
2. Mustafa, R, Hamid, AA, Mohamed, S, Bakar, FA. Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. J Food Sci 2010;75:28–35. https://doi.org/10.1111/j.1750-3841.2009.01401.x.Search in Google Scholar PubMed
3. Uddin, MR, Sinha, S, Hossain, MA, Kaisar, MA, Hossain, MK, Rashid, MA. Chemical and biological investigations of Justicia gendarussa (Burm.f). Dhaka Univ J Pharm Sci 2011;10:53–7.10.3329/dujps.v10i1.10016Search in Google Scholar
4. Paval, J, Kaitheri, SK, Potu, BK, Govindan, S, Kumar, RS, Narayanan, SN, et al.. Anti-arthritic potential of the plant Justicia gendarussa Burm F. Clinics (Sao Paulo) 2009;64:357–62. https://doi.org/10.1590/s1807-59322009000400015.Search in Google Scholar PubMed PubMed Central
5. Kavitha, SK, Viji, V, Kripa, K, Helen, A. Protective effect of Justicia gendarussa Burm.f. on carrageenan-induced inflammation. J Nat Med 2011;65:471–9. https://doi.org/10.1007/s11418-011-0524-z.Search in Google Scholar PubMed
6. Periyanayagam, K, Umamaheswari, B, Suseela, L, Padmini, M, Ismail, M. Evaluation of antiangiogenic effect of the leaves of Justicia gendarussa (Burm.f) (Acanthaceae) by chrio allontoic membrane method. Am J Infect Dis 2009;5:180–2. https://doi.org/10.3844/ajidsp.2009.180.182.Search in Google Scholar
7. Young, RC, Ozols, RF, Myers, CE. The anthracycline antineoplastic drugs. N Engl J Med 1981;305:139–53. https://doi.org/10.1056/nejm198107163050305.Search in Google Scholar
8. Lefrak, EA, Piťha, J, Rosenheim, S, Gottlieb, JA. A clinicopathologic analysis of adriamycin cardiotoxicity. J Cancer 1973;32:302–14. https://doi.org/10.1002/1097-0142(197308)32:2<302::aid-cncr2820320205>3.0.co;2-2.10.1002/1097-0142(197308)32:2<302::AID-CNCR2820320205>3.0.CO;2-2Search in Google Scholar
9. Powis, G. Free radical formation by antitumor quinones. J Free radical biology 1989;6:63–101. https://doi.org/10.1016/0891-5849(89)90162-7.Search in Google Scholar
10. Gutteridge, JM, Halliwell, B. Invited review free radicals in disease processes: a compilation of cause and consequence. Free Radic. Res. Commun. 1993;19:141–58. https://doi.org/10.3109/10715769309111598.Search in Google Scholar
11. Beers, RF, Sizer, IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 1952;195:133–40. https://doi.org/10.1016/s0021-9258(19)50881-x.Search in Google Scholar
12. Ohkawa, H, Ohishi, N, Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. J Anal Biochem 1979;95:351–8. https://doi.org/10.1016/0003-2697(79)90738-3.Search in Google Scholar PubMed
13. Moron, MS, Depierre, JW, Mannervik, B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. J Biochim Biophys Acta Gen Subj 1979;582:67–78. https://doi.org/10.1016/0304-4165(79)90289-7.Search in Google Scholar PubMed
14. Hafemann, SF, Chilman, CS. Implications of vasectomy for social work practice. J Soc Casework 1974;55:343–51. https://doi.org/10.1177/104438947405500602.Search in Google Scholar
15. Lowry, O, Rosebrough, N, Farr, AL, Randall, R. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265–75. https://doi.org/10.1016/s0021-9258(19)52451-6.Search in Google Scholar
16. Ames, BN. Dietary carcinogens and anticarcinogens: oxygen radicals and degenerative diseases. J Science 1983;221:1256–64. https://doi.org/10.1126/science.6351251.Search in Google Scholar PubMed
17. Sun, Y. Free radicals, antioxidant enzymes, and carcinogenesis. J Free Radic Biol Med 1990;8:583–99. https://doi.org/10.1016/0891-5849(90)90156-d.Search in Google Scholar PubMed
18. Lee, V, Randhawa, AK, Singal, PK. Adriamycin-induced myocardial dysfunction in vitro is mediated by free radicals. Am J Physiol Heart Circ Physiol 1991;261:H989–95. https://doi.org/10.1152/ajpheart.1991.261.4.h989.Search in Google Scholar PubMed
19. Kitakaze, M. Doxorubicin, to treat cancers or to cause cardiac injury, that is the question. Cardiovasc Drugs Ther 2001;15:199. https://doi.org/10.1023/a:1011968903148.10.1023/A:1011968903148Search in Google Scholar PubMed
20. Agapito, MT, Antolín, Y, Del Brio, MT, López‐Burillo, S, Pablos, MI, Recio, JM. Protective effect of melatonin against adriamycin toxicity in the rat. J Pineal Res 2001;31:23–30. https://doi.org/10.1034/j.1600-079x.2001.310104.x.Search in Google Scholar PubMed
21. Xu, MF, Tang, PL, Qian, ZM, Ashraf, M. Effects by doxorubicin on the myocardium are mediated by oxygen free radicals. J Life Sci 2001;68:889–901. https://doi.org/10.1016/s0024-3205(00)00990-5.Search in Google Scholar PubMed
22. Garcı́a, JJ, Reiter, RJ, Guerrero, JM, Escames, G, Byung, PY, Oh, CS, et al.. Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation. J FEBS Lett 1997;408:297–300. https://doi.org/10.1016/s0014-5793(97)00447-x.Search in Google Scholar PubMed
23. Bachur, NR, Gordon, SL, Gee, MV. A general mechanism for microsomal activation of quinone anticancer agents to free radicals. J Cancer Res 1978;38:1745–50.Search in Google Scholar
24. Kalyanaraman, B, Sealy, RC, Sinha, B. An electron spin resonance study of the reduction of peroxides by anthracycline semiquinones Biochim Biophys Acta Gen Subj 1984;799:270–5. https://doi.org/10.1016/0304-4165(84)90270-8.Search in Google Scholar PubMed
25. Olson, RD, Boerth, RC, Gerber, JG, Nies, AS. Mechanism of adriamycin cardiotoxicity: evidence for oxidative stress. J Life Sci 1981;29:1393–401. https://doi.org/10.1016/0024-3205(81)90001-1.Search in Google Scholar PubMed
26. Zipper, J. Proliferation of myocardial peroxisomes caused by several agents and conditions. J Mol Cell Cardiol 1997;29:149–61. https://doi.org/10.1006/jmcc.1996.0260.Search in Google Scholar PubMed
27. Adachi, T, Nagae, T, Ito, Y, Hirano, K, Sugiura, M. Relation between cardiotoxic effect of adriamycin and superoxide anion radical. J Pharmacobio-Dyn 1983;6:114–23. https://doi.org/10.1248/bpb1978.6.114.Search in Google Scholar PubMed
28. Gustafson, DL, Swanson, JD, Pritsos, CA. Modulation of glutathione and glutathione dependent antioxidant enzymes in mouse heart following doxorubicin therapy. Free Radic Res Commun 1993;19:111–20. https://doi.org/10.3109/10715769309056505.Search in Google Scholar PubMed
29. Li, T, Danelisen, I, Belló-Klein, A, Singal, PK. Effects of probucol on changes of antioxidant enzymes in adriamycin-induced cardiomyopathy in rats. J Cardiovasc Res 2000;46:523–30. https://doi.org/10.1016/s0008-6363(00)00039-0.Search in Google Scholar PubMed
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Articles in the same Issue
- Frontmatter
- Editorials
- Deiodination and tumor progression: the interplay between thyroid hormones intracellular activation and the androgen signal
- The intricate role of glutamine in pathophysiological contexts
- Reviews
- Zebrafish as a model organism – can a fish mimic human?
- Phytocompounds and their molecular targets in immunomodulation: a review
- Original Articles
- Host–parasite relationship modulates the effect of African mistletoe leaves on the cholinergic, monoaminergic and carbohydrate hydrolyzing enzymes in fruit fly
- Behavioral and biochemical investigations to explore the efficacy of quercetin and folacin in experimental diabetes induced vascular endothelium dysfunction and associated dementia in rats
- Chronic exposure of industrial grade calcium carbide and ethylene glycol exert genotoxic effect in Wistar albino rats
- A four-year review of uterine rupture at a secondary health facility in Okitipupa, Southwest Nigeria
- Increased nitric oxide availability worsens the cardiac performance during early re-perfusion period in adult rats
- Safety evaluation of an antimalarial herbal product from Andrographis paniculata (AS201-01) in healthy volunteers
- Tobacco use and clinical leukoplakia lesions among south Indian tribes
- Carcinogen sodium arsenite disrupts antioxidant and redox homeostasis in Drosophila melanogaster
- No association of the common Asian mitochondrial DNA haplogroups with lung cancer in East Indian population
- Cardioprotective effect of Justicia gendarussa on doxorubicin induced toxicity in mice
- Lung ultrasound-guided PEEP titration in COVID–19 patients treated with CPAP
- Short Communication
- Effects of the gaseous signalling molecule nitroxyl (HNO) on myenteric neurons governing intestinal motility
Articles in the same Issue
- Frontmatter
- Editorials
- Deiodination and tumor progression: the interplay between thyroid hormones intracellular activation and the androgen signal
- The intricate role of glutamine in pathophysiological contexts
- Reviews
- Zebrafish as a model organism – can a fish mimic human?
- Phytocompounds and their molecular targets in immunomodulation: a review
- Original Articles
- Host–parasite relationship modulates the effect of African mistletoe leaves on the cholinergic, monoaminergic and carbohydrate hydrolyzing enzymes in fruit fly
- Behavioral and biochemical investigations to explore the efficacy of quercetin and folacin in experimental diabetes induced vascular endothelium dysfunction and associated dementia in rats
- Chronic exposure of industrial grade calcium carbide and ethylene glycol exert genotoxic effect in Wistar albino rats
- A four-year review of uterine rupture at a secondary health facility in Okitipupa, Southwest Nigeria
- Increased nitric oxide availability worsens the cardiac performance during early re-perfusion period in adult rats
- Safety evaluation of an antimalarial herbal product from Andrographis paniculata (AS201-01) in healthy volunteers
- Tobacco use and clinical leukoplakia lesions among south Indian tribes
- Carcinogen sodium arsenite disrupts antioxidant and redox homeostasis in Drosophila melanogaster
- No association of the common Asian mitochondrial DNA haplogroups with lung cancer in East Indian population
- Cardioprotective effect of Justicia gendarussa on doxorubicin induced toxicity in mice
- Lung ultrasound-guided PEEP titration in COVID–19 patients treated with CPAP
- Short Communication
- Effects of the gaseous signalling molecule nitroxyl (HNO) on myenteric neurons governing intestinal motility
