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Acetylcholinesterase inhibitory activity of extract and fractions from the root of Rauvolfia serpentina(L.) Bth.ex Kurz

  • Suciati EMAIL logo , Debora Poerwantoro , Aty Widyawaruyanti and Kornkanok Ingkaninan
Published/Copyright: June 25, 2021
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 32 Issue 4

Abstract

Objectives

Alzheimer’s disease (AD) is a degenerative brain disease characterized by confusion, behavior changes, decline in memory and cognitive skills. One of the strategies in the treatment of AD is to use acetylcholinesterase (AChE) inhibitors. The current study aims to determine the AChE inhibitory activities of the extract and fractions of the root of Rauvolfia serpentina.

Methods

Extraction was carried out by maceration method using ethanol, followed by liquid–liquid partition using n-hexane, ethyl acetate and n-butanol. Further fractionation was conducted by using vacuum liquid chromatography (VLC). The AChE inhibitory assays were performed by using Ellmann’s method. Phytochemical screening was carried out by TLC method.

Results

The ethanolic extract of R. serpentina showed inhibition against AChE enzyme with an IC50 value of 7.46 μg/mL. The extract and fractions showed higher inhibition against butyrylcholinesterase (BChE) compared to AChE. Amongst three fractions obtained, the n-butanol fraction showed the strongest inhibition with an IC50 value of 5.99 μg/mL against AChE. VLC fractionation of the n-butanol fraction yielded 13 subfractions (VLC 1–VLC 13). Four out of 13 subfractions gave more than 80% inhibition against AChE, namely subfractions 4–7, with IC50 values ranging from 4.87 to 47.22 μg/mL. The phytochemical screening of these subfractions suggested the presence of alkaloids.

Conclusions

The ethanolic extract, as well as fractions of R. serpentina root, are potential for AChE inhibitor. The alkaloid compound may be responsible for this activity.

Keywords: acetylcholinesterase inhibitor; Alzheimer’s disease; Rauvolfia serpentina
Corresponding author: Suciati, Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia; and Natural Product Medicine Research and Development, Institute of Tropical Diseases, Universitas Airlangga, Surabaya, Indonesia, E-mail:

Funding source: Ministry of Research, Technology, and Higher Education of the Republic of Indonesia

Award Identifier / Grant number: PDUPT 2020

  1. Research funding: This research was funded by the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia through PDUPT 2020 research grant.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: Not applicable.

References

1. Murray, AP, Faraoni, MB, Castro, MJ, Alza, NP, Cavallaro, V. Natural AChE inhibitors from plants and their contribution to Alzheimer’s disease therapy. Curr Neuropharmacol 2013;11:388–413. https://doi.org/10.2174/1570159x11311040004.Search in Google Scholar

2. Thakur, AK, Kamboj, P, Goswami, K. Pathophysiology and management of Alzheimer’s disease: an overview. J Anal Pharm Res 2018;9:226–35.10.15406/japlr.2018.07.00230Search in Google Scholar

3. Bird, TD, Miller, BL. Alzheimer’s disease and primary dementias. In: Fauci, AS, Braunwald, E, editors. Harrison’s principles of internal medicine, 17th ed. New York: McGraw-Hill; 2008.Search in Google Scholar

4. Dev, K, Maurya, R. Marine-derived anti-Alzheimer’s agents of promise. In: Brahmachari, G, editor. Neuroprotective natural products, clinical aspects and mode of action. Weinheim: Wiley VCH; 2017.10.1002/9783527803781.ch7Search in Google Scholar

5. Mathew, M, Subramanian, S. In vitro screening for anti-cholinesterase and antioxidant activity of methanolic extracts of ayurvedic medicinal plants used for cognitive disorders. PloS One 2014;9:e86804. https://doi.org/10.1371/journal.pone.0086804.Search in Google Scholar

6. Hostettmann, K, Borloz, A, Urbain, A, Marston, A. Natural product inhibitors of acetylcholinesterase. Curr Org Chem 2006;10:825–47. https://doi.org/10.2174/138527206776894410.Search in Google Scholar

7. Lleo, A, Greenberg, SM, Growdon, JH. Current pharmacotherapy for Alzheimer’s disease. Annu Rev Med 2006;57:513–33. https://doi.org/10.1146/annurev.med.57.121304.131442.Search in Google Scholar

8. Chopra, K, Misra, S, Kuhad, A. Current perspectives on pharmacotherapy of Alzheimer’s disease. Expet Opin Pharmacother 2011;12:335–50. https://doi.org/10.1517/14656566.2011.520702.Search in Google Scholar

9. Suciati Rabgay, K, Fachrunniza, Y, Saesong, T, Hadi, TA, Wahyuni, TS, Widyawaruyanti, A, et al.. Enzyme inhibitory activities of marine sponges against cholinesterase and 5α-reductase. Malays Appl Biol 2019;48:77–83.Search in Google Scholar

10. Suciati, Laili, ER, Poerwantoro, D, Hapsari, AP, Gifanda, LZ, Rabgay, K, et al.. Evaluation of cholinesterase inhibitory activity of six Indonesian Cassia species. J Res Pharm 2020;24:472–8. https://doi.org/10.35333/jrp.2020.195.Search in Google Scholar

11. World Health Organization. WHO monographs on selected medicinal plants. Geneva: World Health Organization; 1999, vol 1.Search in Google Scholar

12. Poonam, AS, Mishra, S. Physiological, biochemical and modern biotechnological approach to improvement of Rauwolfia serpentina. J Pharm Biol Sci 2013;6:73–8. https://doi.org/10.9790/3008-0627378.Search in Google Scholar

13. Ruyter, CM, Akram, M, Illahi, I, Stöckigt, J. Investigation of the alkaloid content of Rauwolfia serpentina roots from regenerated plants. Planta Med 1991;57:328–30. https://doi.org/10.1055/s-2006-960109.Search in Google Scholar

14. Singh, M, Kaur, R, Rajput, R, Mathur, G. Evaluating the therapeutic efficiency and drug targeting ability of alkaloids present in Rauwolfia serpentina. Int J Green Pharm 2017;11:132–42.Search in Google Scholar

15. Lobay, D. Rauwolfia in the treatment of hypertension. Integr Med 2015;14:40–6.Search in Google Scholar

16. Ellman, GL, Courtney, KD, Andres, VJr, Featherstone, RM. Anew and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961;7:88–95. https://doi.org/10.1016/0006-2952(61)90145-9.Search in Google Scholar

17. Houghton, PJ, Ren, Y, Howes, MJ. Acetylcholinesterase inhibitors from plants and fungi. Nat Prod Rep 2006;23:181–99. https://doi.org/10.1039/b508966m.Search in Google Scholar

18. Orhan, I, Sener, B, Choudhary, MI, Khalid, A. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of some Turkish medicinal plants. J Ethnopharmacol 2004;91:57–60. https://doi.org/10.1016/j.jep.2003.11.016.Search in Google Scholar

19. Zhao, T, Ding, KM, Zhang, L, Cheng, XM, Wang, CH, Wang, ZT. Acetylcholinesterase and butyrylcholinesterase inhibitory activities of β-carboline and quinoline alkaloids derivatives from the plants of genus Peganum. J Chem 2013;2013:1–6. https://doi.org/10.1155/2013/717232.Search in Google Scholar

20. Ahmed, F, Ghalib, RM, Sasikala, P, Ahmed, KK. Cholinesterase inhibitors from botanicals. Phcog Rev 2013;7:121–30. https://doi.org/10.4103/0973-7847.120511.Search in Google Scholar

21. Fadaeinasab, M, Basiri, A, Kia, Y, Karimian, H, Ali, HM, MurugaiyahV. New indole alkaloids from the bark of Rauvolfia reflexa and their cholinesterase inhibitory activity. Cell Physiol Biochem 2015;37:1997–2011. https://doi.org/10.1159/000438560.Search in Google Scholar

22. Passos, CS, Simoes-Pires, CAS, Nurisso, A, Soldi, TC, Kato, L, de Oliveira, CMA, et al.. Indole alkaloids of Psychotria as multifunctional cholinesterases and monoamine oxidases inhibitors. Phytochemistry 2013;86:8–20. https://doi.org/10.1016/j.phytochem.2012.11.015.Search in Google Scholar
Received: 2020-11-26
Accepted: 2021-01-26
Published Online: 2021-06-25

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jbcpp-2020-0401
Keywords for this article
acetylcholinesterase inhibitor; Alzheimer’s disease; Rauvolfia serpentina

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Cost of illness of diabetes mellitus in Indonesia: a systematic review
  4. Social media health interventions to improve diabetes mellitus patient outcome: a systematic review
  5. Developing pharmacokinetics–pharmacodynamics model of valproic acid syrup based on prediction of population pharmacokinetics parameter and seizure frequency in Indonesian pediatric epilepsy outpatients
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  7. Green tea and its active compound epigallocathechin-3-gallate (EGCG) inhibit neuronal apoptosis in a middle cerebral artery occlusion (MCAO) model
  8. The effects of quercetin on nicotine-induced reward effects in mice
  9. Resveratrol ameliorates physical and psychological stress-induced depressive-like behavior
  10. Translation and cross-cultural adaption of an instrument measuring patient’s well-being under treatment for schizophrenia
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  80. Cratoxylum sumatranum stem bark exhibited antimalarial activity by Lactate Dehydrogenase (LDH) assay
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  83. In vitro antimalarial activity of Garcinia parvifolia Miq. Stem extracts and fractions on Plasmodium falciparum lactate dehydrogenase (LDH) assay
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  89. In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent
  90. Inhibitory activity of Urena lobata leaf extract on alpha-amylase and alpha-glucosidase: in vitro and in silico approach
  91. Case Report
  92. Effect of hydrocortisone on hypocorticolism caused by pituitary adenoma
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