Carcinogen sodium arsenite disrupts antioxidant and redox homeostasis in Drosophila melanogaster

Aghogho Oyibo; Amos O. Abolaji; Oyeronke A. Odunola

doi:10.1515/jbcpp-2020-0235

Article

Carcinogen sodium arsenite disrupts antioxidant and redox homeostasis in Drosophila melanogaster

Aghogho Oyibo , Amos O. Abolaji and Oyeronke A. Odunola

Published/Copyright: August 4, 2021

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From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 34 Issue 5

Abstract

Objectives

The inadvertent exposure to environmental contaminants has been reported to induce cancer in different animal models. Here, we investigated the toxicity of Sodium Arsenite (SA), a Class I Carcinogen in Drosophila melanogaster.

Methods

Harwich fly strain (1–3 days old) of both sexes were orally exposed to SA (0, 0.0312, 0.0625 and 0.125 mM) for 14 days for survival study. Thereafter, 5 days exposure period was selected to assess the toxic effects of SA on oxidative stress and antioxidant markers.

Results

The results indicated that SA induced significant reduction in survival and emergence rate of flies. Furthermore, SA significantly increased Nitric Oxide (NO, nitrite and nitrate) and Hydrogen Peroxide (H₂O₂) levels in flies compared with control (p<0.05). In addition, SA inhibited catalase and glutathione-S-transferase (GST) activities, and depleted total thiol and glutathione (GSH) contents. Moreover, acetylcholinesterase activity significantly increased in flies treated with SA when compared with control.

Conclusions

Sodium arsenite-induced reduction in survival and emergence rates of flies occurred via the disruption of oxidative stress-antioxidant homeostasis in D. melanogaster.

Keywords: antioxidant status; Drosophila melanogaster ; environmental carcinogen; oxidative stress; sodium arsenite

Corresponding authors: Professor Oyeronke A. Odunola, Cancer Research and Molecular Biology Laboratories, Department of Biochemistry , Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, 200284, Ibadan, Nigeria, Phone: +2348035266802, E-mail: ronodunola@yahoo.com; and Dr. Amos O. Abolaji, Drosophila Laboratory, Department of Biochemistry, Molecular Drug Metabolism and Toxicology Unit, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, 200284, Ibadan, Nigeria, Phone: +2348068614194, E-mail: ao.abolaji@ui.edu.ng

Funding source: University of Ibadan 10.13039/501100004918

Acknowledgments

We acknowledge the Postgraduate School, University of Ibadan, Nigeria for the research scholarship to Aghogho Oyibo.

Research funding: The author Aghogho Oyibo was supported by the research scholarship from Postgraduate School, University of Ibadan, Nigeria.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all individuals included in this study.
Ethical approval: There is currently no ethical restriction to the use of D. melanogaster.

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Received: 2020-08-16

Accepted: 2021-07-08

Published Online: 2021-08-04

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Articles in the same Issue

https://doi.org/10.1515/jbcpp-2020-0235

Keywords for this article

antioxidant status; Drosophila melanogaster; environmental carcinogen; oxidative stress; sodium arsenite