Ovalbumin/lipopolysaccharide induced vasculitis in rats: a new predictive model
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Vandana R. Thakur
Abstract
Objectives
Currently, there are several animal models for vasculitis. Ovalbumin and lipopolysaccharide (OVA, LPS) are well established for causing inflammation and used as an adjunct in the vasculitis induction. However, to date, none has established the effect of OVA and LPS in disease induction. Therefore, in the present study, an attempt has been made to develop a new animal model for vasculitis using OVA/LPS in rats.
Methods
A total of 42 Wistar rats were divided randomly into seven groups (n=6/group), normal control, and three different doses (0.5, 1, and 5 mg/kg) of OVA and LPS treated groups. Half of the rats in each group received only intraperitoneal sensitization, while the remaining half rats were additionally subjected to a one-week intranasal challenge.
Results
Results showed that both OVA/LPS in their respective groups have significantly increased circulating inflammatory cells, C-reactive protein (CRP), Inflammatory cytokines (IL-1β, IL-6, TNF-α), Kidney damage markers (BUN, Creatinine), and liver function enzymes (AST, ALT) in a dose-dependent manner.
Conclusions
OVA/LPS induced vascular inflammation in a dose-dependent manner. However, the higher (5 mg/kg) dose of ovalbumin and lipopolysaccharide has contributed to severe vascular inflammation through increasing inflammatory cytokines. These findings suggest that OVA/LPS may contribute as a possible model for vasculitis in rats.
Funding source: Student Startup Innovation Policy (SSIP) scheme, Government of Gujarat, India
Award Identifier / Grant number: Grant Number 215/2019
Acknowledgments
None.
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Research funding: This work was supported by grant from Student Startup Innovation Policy (SSIP) scheme (Grant Number 215/2019), Government of Gujarat, India.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: NA.
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Ethical approval: Experimental protocols for this study was reviewed and approved by the Institutional Animal Ethical Committee (IAEC), (LMCP/P’Cology/18/03) and adhered to the guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Environment and Forest, Government of India.
References
1. Sharma, P, Sharma, S, Baltaro, R, Hurley, J. Systemic vasculitis. Am Fam Physician 2011;83:556–65.Search in Google Scholar
2. Sneller, MC, Fauci, AS. Pathogenesis of vasculitis syndromes. Med Clin N Am 1997;81:221–42. https://doi.org/10.1016/s0025-7125(05)70512-5.Search in Google Scholar
3. Guillevin, L, Dörner, T. Vasculitis: mechanisms involved and clinical manifestations. Arthritis Res Ther 2007;9(2 Suppl):S9. https://doi.org/10.1186/ar2193.Search in Google Scholar
4. Wilde, B, Van Paassen, P, Witzke, O, Tervaert, JWC. New pathophysiological insights and treatment of ANCA-associated vasculitis. Kidney Int 2011;79:599–612. https://doi.org/10.1038/ki.2010.472.Search in Google Scholar
5. Ballanti, E, Chimenti, MS, Perricone, R. Small-medium vessel vasculitides: is the complement System a potential forgotten target? Isr Med Assoc J 2015;17:85–92.Search in Google Scholar
6. Buckley, CD, Rainger, GE, Nash, GB, Raza, K. Endothelial cells, fibroblasts and vasculitis. Rheumatology (Oxford) 2005;44:860–63. https://doi.org/10.1093/rheumatology/keh542.Search in Google Scholar
7. Kanemitsu, H, Matsunawa, M, Wakabayashi, K, Sato, M, Takahashi, R, Odai, T, et al.. Increased serum levels of macrophage migration inhibitory factor (MIF) in patients with microscopic polyangiitis. Open Access Rheumatol 2009;1:1–8. https://doi.org/10.2147/oarrr.s4906.Search in Google Scholar
8. Huugen, D, Xiao, H, Van Esch, A, Falk, RJ, Peutz-Kootstra, CJ, Buurman, WA, et al.. Aggravation of anti-myeloperoxidase antibody-induced glomerulonephritis by bacterial lipopolysaccharide: role of tumor necrosis factor-alpha. Am J Pathol 2005;167:47–58. https://doi.org/10.1016/s0002-9440(10)62952-5.Search in Google Scholar
9. Ooi, JD, Chang, J, Hickey, MJ, Borza, DB, Fugger, L, Holdsworth, SR, et al.. The immunodominant myeloperoxidase T-cell epitope induces local cell-mediated injury in antimyeloperoxidase glomerulonephritis. Proc Natl Acad Sci U S A 2012;109:E2615–24. https://doi.org/10.1073/pnas.1210147109.Search in Google Scholar PubMed PubMed Central
10. Yu, QL, Chen, Z. Establishment of different experimental asthma models in mice. Exp Ther Med 2018;15:2492–98. https://doi.org/10.3892/etm.2018.5721.Search in Google Scholar PubMed PubMed Central
11. Kim, YK, Oh, SY, Jeon, SG, Park, HW, Lee, SY, Chun, EY, et al.. Airway exposure levels of lipopolysaccharide determine type 1 versus type 2 experimental asthma. J Immunol 2007;178:5375–82. https://doi.org/10.4049/jimmunol.178.8.5375.Search in Google Scholar PubMed
12. Sharif, MK, Saleem, M, Javed, K. Role of materials science in food bioengineering. In: Grumezescu, A, Holban, A, editors. Handbook of food bioengineering. Cambridge: Academic Press; 2018:505–37 pp.10.1016/B978-0-12-811448-3.00015-2Search in Google Scholar
13. Rahman, A, Kefer, J, Bando, M, Niles, WD, Malik, AB. E-selectin expression in human endothelial cells by TNF-alpha-induced oxidant generation and NF-kappaB activation. Am J Physiol 1998;275:L53–44. https://doi.org/10.1152/ajplung.1998.275.3.l533.Search in Google Scholar
14. Marshak-Rothstein, A. Toll-like receptors in systemic autoimmune disease. Nat Rev Immunol 2006;6:823–35. https://doi.org/10.1038/nri1957.Search in Google Scholar
15. Metzler, KD, Goosmann, C, Lubojemska, A, Zychlinsky, A, Papayannopoulos, V. A myeloperoxidase-containing complex regulates neutrophil elastase release and actin dynamics during NETosis. Cell Rep 2014;8:883–96. https://doi.org/10.1016/j.celrep.2014.06.044.Search in Google Scholar
16. Liu, T, Zhang, L, Joo, D, Sun, SC. NF-κB signaling in inflammation. Sig Transduct Target Ther 2017;2:17023. https://doi.org/10.1038/sigtrans.2017.23.Search in Google Scholar
17. Miraghazadeh, B, Cook, M. Nuclear factor-kappaB in autoimmunity: man and mouse. Front Immunol 2018;9:613. https://doi.org/10.3389/fimmu.2018.00613.Search in Google Scholar
18. Schönermarck, U, Csernok, E, Gross, WL. Pathogenesis of anti-neutrophil cytoplasmic antibody-associated vasculitis: challenges and solutions. Nephrol Dial Transplant 2014;30:i46–52.10.1093/ndt/gfu398Search in Google Scholar
19. Mohammad, AJ, Hot, A, Arndt, F, Moosig, F, Guerry, MJ, Amudala, N, et al.. Rituximab for the treatment of eosinophilic granulomatosis with polyangiitis (Churg-Strauss). Ann Rheum Dis 2016;75:396–401. https://doi.org/10.1136/annrheumdis-2014-206095.Search in Google Scholar
20. Roane, DW, Griger, DR. An approach to diagnosis and initial management of systemic vasculitis. Am Fam Physician 1999;60:1421–30.10.1097/00124743-199904000-00005Search in Google Scholar
21. Mandell, BF, Hoffman, GS. Differentiating the vasculitides. Rheum Dis Clin N Am 1994;20:409–42.10.1016/S0889-857X(21)00056-9Search in Google Scholar
22. Sundy, JS, Haynes, BF. Cytokines and adhesion molecules in the pathogenesis of vasculitis. Curr Rheumatol Rep 2000;2:402–10. https://doi.org/10.1007/s11926-000-0040-8.Search in Google Scholar PubMed
23. Zhang, H, Park, Y, Wu, J, Chen, XP, Lee, S, Yang, J, et al.. Role of TNF-alpha in vascular dysfunction. Clin Sci (Lond). 2009;116:219–30. https://doi.org/10.1042/cs20080196.Search in Google Scholar PubMed PubMed Central
24. Hsu, T, Nguyen-Tran, HH, Trojanowska, M. Active roles of dysfunctional vascular endothelium in fibrosis and cancer. J Biomed Sci 2019;26:86. https://doi.org/10.1186/s12929-019-0580-3.Search in Google Scholar PubMed PubMed Central
25. Schersten, H, Kirno, K, Ekroth, R, Lundin, S, Pettersson, A, Kjellstrom, C, et al.. Impaired endothelium-mediated vasodilatation in the peripheral vasculature of patients with acute pulmonary allograft rejection. J Heart Lung Transplant 1996;15:556–63.Search in Google Scholar
26. Wildhirt, SM, Weis, M, Schulze, C, Conrad, N, Pehlivanli, S, Rieder, G, et al.. Expression of endomyocardial nitric oxide synthase and coronary endothelial function in human cardiac allografts. Circulation 2001;104(1 Suppl):336–43. https://doi.org/10.1161/hc37t1.094598.Search in Google Scholar PubMed
27. Yamaguchi, M, Ando, M, Kato, S. Increase of antimyeloperoxidase antineutrophil cytoplasmic antibody (ANCA) in patients with renal ANCA-associated vasculitis: association with risk to relapse. J Rheumatol 2015;42:1853–60. https://doi.org/10.3899/jrheum.141622.Search in Google Scholar PubMed
28. Cojocaru, M, Cojocaru, IM, Silosi, I, Vrabie, CD. Liver involvement in patients with systemic autoimmune diseases. Maedica (Buchar). 2013;8:394–97.Search in Google Scholar
© 2021 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
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- Assessment of sleep quality and its predictors among newly diagnosed psychiatric patients
- Ovalbumin/lipopolysaccharide induced vasculitis in rats: a new predictive model
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- Effect of luteolin on the gene level expression of apoptosis-associated speck-like protein containing a caspase recruitment domain of NLRP3 inflammasome and NF-κB in rats subjected to experimental pancreatitis – influence of HSP70
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- In vitro anticoagulant activity of selected medicinal plants: potential interactions with warfarin and development of new anticoagulants
- Normal reference value of orthodromic and antidromic sensory nerve conduction velocity of median nerve with intact palmaris longus tendon in apparently healthy individuals
- Short Communication
- Chemokine receptor antagonists with α1-adrenergic receptor blocker activity
Articles in the same Issue
- Frontmatter
- Minireview
- Can pulse wave velocity (PWV) alone express arterial stiffness? A neglected tool for vascular function assessment
- Review
- Hyponatremia in heart failure: not just 135 to 145
- Original Articles
- Effects of berberine on cholinesterases and monoamine oxidase activities, and antioxidant status in the brain of streptozotocin (STZ)-induced diabetic rats
- Paradoxical sleep deprivation induces oxidative stress in the submandibular glands of Wistar rats
- Terminalia arjuna supplementation ameliorates high fat diet-induced oxidative stress in nephrotoxic rats
- Decreased expression of annexin A2 and loss of its association with vascular endothelial growth factor leads to the deficient trophoblastic invasion in preeclampsia
- Algogen-induced vasosensory reflexes modulate short-term heart rate variability parameters in experimental rat models
- Assessment of sleep quality and its predictors among newly diagnosed psychiatric patients
- Ovalbumin/lipopolysaccharide induced vasculitis in rats: a new predictive model
- Dose-dependent and time-dependent metabolic, hemodynamic, and redox disturbances in dexamethasone-treated Wistar rats
- Effects of sleeve gastrectomy on neutrophil–lymphocyte ratio
- Effect of luteolin on the gene level expression of apoptosis-associated speck-like protein containing a caspase recruitment domain of NLRP3 inflammasome and NF-κB in rats subjected to experimental pancreatitis – influence of HSP70
- Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line
- Feasibility, face, and content validity of quantitative computed tomography in interstitial lung disease related to connective tissue diseases
- In vitro anticoagulant activity of selected medicinal plants: potential interactions with warfarin and development of new anticoagulants
- Normal reference value of orthodromic and antidromic sensory nerve conduction velocity of median nerve with intact palmaris longus tendon in apparently healthy individuals
- Short Communication
- Chemokine receptor antagonists with α1-adrenergic receptor blocker activity
