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Illuminating the brain-genetically encoded single wavelength fluorescent biosensors to unravel neurotransmitter dynamics

  • Martin Kubitschke and Olivia A. Masseck ORCID logo EMAIL logo
Published/Copyright: May 30, 2023
Biological Chemistry
From the journal Biological Chemistry Volume 405 Issue 1

Abstract

Understanding how neuronal networks generate complex behavior is one of the major goals of Neuroscience. Neurotransmitter and Neuromodulators are crucial for information flow between neurons and understanding their dynamics is the key to unravel their role in behavior. To understand how the brain transmits information and how brain states arise, it is essential to visualize the dynamics of neurotransmitters, neuromodulators and neurochemicals. In the last five years, an increasing number of single-wavelength biosensors either based on periplasmic binding proteins (PBPs) or on G-protein-coupled receptors (GPCR) have been published that are able to detect neurotransmitter release in vitro and in vivo with high spatial and temporal resolution. Here we review and discuss recent progress in the development of these sensors, their limitations and future directions.

Keywords: cpGFP; GPCRs; neurochemicals; neuromodulators; neurotransmission; PBPs
Corresponding author: Olivia A. Masseck, Synthetic Biology, University of Bremen, 28359 Bremen, Germany, E-mail:

Funding source: DFG MA 4692/6

Funding source: DFG 122679504 - SFB 874

Acknowledgment

We would like to thank Juliana Groß and Kim Renken for their valuable comments and proof reading.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work was funded by DFG (Nos. MA 4692/6, 122679504 - SFB 874).

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2023-04-05
Accepted: 2023-05-15
Published Online: 2023-05-30
Published in Print: 2024-01-29

© 2023 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Highlight: Horizons in Neuroscience - Organoids, Optogenetics and Remote Control
  3. Highlight: Horizons in Neuroscience – Organoids, Optogenetics and Remote Control
  4. Towards correlative archaeology of the human mind
  5. The promise of genetic screens in human in vitro brain models
  6. Schwann cells in neuromuscular in vitro models
  7. Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy
  8. Optogenetics 2.0: challenges and solutions towards a quantitative probing of neural circuits
  9. Illuminating the brain-genetically encoded single wavelength fluorescent biosensors to unravel neurotransmitter dynamics
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https://doi.org/10.1515/hsz-2023-0175
Keywords for this article
cpGFP; GPCRs; neurochemicals; neuromodulators; neurotransmission; PBPs

Articles in the same Issue

  1. Frontmatter
  2. Highlight: Horizons in Neuroscience - Organoids, Optogenetics and Remote Control
  3. Highlight: Horizons in Neuroscience – Organoids, Optogenetics and Remote Control
  4. Towards correlative archaeology of the human mind
  5. The promise of genetic screens in human in vitro brain models
  6. Schwann cells in neuromuscular in vitro models
  7. Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy
  8. Optogenetics 2.0: challenges and solutions towards a quantitative probing of neural circuits
  9. Illuminating the brain-genetically encoded single wavelength fluorescent biosensors to unravel neurotransmitter dynamics
  10. Microtubules as a signal hub for axon growth in response to mechanical force
  11. The good or the bad: an overview of autoantibodies in traumatic spinal cord injury
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