Abstract
Neuromuscular cell culture models are used to investigate synapse formation and function, as well as mechanisms of de-and regeneration in neuromuscular diseases. Recent developments including 3D culture technique and hiPSC technology have propelled their ability to complement insights from in vivo models. However, most cultures have not considered Schwann cells, the glial part of NMJs. In the following, a brief overview of different types of neuromuscular cocultures is provided alongside examples for studies that included Schwann cells. From these, findings concerning the effects of Schwann cells on those cultures are summarized and future lines of research are proposed.
Funding source: Bundesministerium für Bildung und Forschung
Award Identifier / Grant number: 03FH8I02IA
Award Identifier / Grant number: 13FH8I05IA
Award Identifier / Grant number: 13FH8I09IA
Funding source: Deutsche Forschungsgemeinschaft
Award Identifier / Grant number: INST 874/9-1
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: S.J.H. was funded by a fellowship of the Albert und Anneliese Konanz-Stiftung. This work was funded by the German Federal Ministry of Education and Research (BMBF) as part of the Innovation Partnership M2Aind, projects M2OGA (03FH8I02IA), Drugs4Future (13FH8I05IA), and DrugsData (13FH8I09IA) within the framework Starke Fachhochschulen—Impuls für die Region (FH-Impuls). This work was supported by Deutsche Forschungsgemeinschaft (DFG), grant INST 874/9-1.
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Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
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Articles in the same Issue
- Frontmatter
- Highlight: Horizons in Neuroscience - Organoids, Optogenetics and Remote Control
- Highlight: Horizons in Neuroscience – Organoids, Optogenetics and Remote Control
- Towards correlative archaeology of the human mind
- The promise of genetic screens in human in vitro brain models
- Schwann cells in neuromuscular in vitro models
- Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy
- Optogenetics 2.0: challenges and solutions towards a quantitative probing of neural circuits
- Illuminating the brain-genetically encoded single wavelength fluorescent biosensors to unravel neurotransmitter dynamics
- Microtubules as a signal hub for axon growth in response to mechanical force
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Articles in the same Issue
- Frontmatter
- Highlight: Horizons in Neuroscience - Organoids, Optogenetics and Remote Control
- Highlight: Horizons in Neuroscience – Organoids, Optogenetics and Remote Control
- Towards correlative archaeology of the human mind
- The promise of genetic screens in human in vitro brain models
- Schwann cells in neuromuscular in vitro models
- Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy
- Optogenetics 2.0: challenges and solutions towards a quantitative probing of neural circuits
- Illuminating the brain-genetically encoded single wavelength fluorescent biosensors to unravel neurotransmitter dynamics
- Microtubules as a signal hub for axon growth in response to mechanical force
- The good or the bad: an overview of autoantibodies in traumatic spinal cord injury