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CD45RO regulates the HIV-1 gp120-mediated apoptosis of T cells by activating Lck

  • Kelei Li , Zhe Cong , Zhuoying Peng , Ting Chen , Jing Xue EMAIL logo and Qiang Wei EMAIL logo
Published/Copyright: March 17, 2018

Abstract

CD45 has been reported to regulate the HIV-1 gp120-induced apoptosis of Jurkat cells. Here, we demonstrate that the extracellular domain of CD45 plays an important role in this function. We observed that CD45RO-transfected cells, but not cells transfected with other CD45 isoforms, underwent significant apoptosis induced by gp120. However, a CD45RA-transfected cell line treated with an O-glycan inhibitor was able to undergo apoptosis. The role of the extracellular domain of CD45 was further confirmed using CD45 isoform-transfected cell lines by analyzing the phosphorylation of Lck, which is a direct substrate of CD45 tyrosine phosphatase, and by using an Lck inhibitor. These results suggest that CD45RO modulates HIV-1 gp120-induced apoptosis by regulating the activity of Lck.

Acknowledgments

We thank Dr. Didier Trono for providing the lentiviral expression vector pWPXL. This work was supported by a grant (2017ZX10304402-001-010) from the National Science and Technology Major Projects of Infectious Disease and by a grant (No. 2017-I2M-1-014) from the Foundation of China and CAMS Innovation Fund for Medical Sciences.

  1. Conflict of interest statement: The authors have no conflicts of interest to declare.

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Received: 2017-7-31
Accepted: 2018-2-28
Published Online: 2018-3-17
Published in Print: 2018-5-24

©2018 Walter de Gruyter GmbH, Berlin/Boston

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