Abstract
Non-small cell lung cancer (NSCLC) is a common malignant tumor. Although the abnormal expression and potential clinical prognostic value of histone deacetylase 1 (HDAC1) were recently discovered in many kinds of cancer, the roles and molecular mechanisms of HDAC1 in NSCLC is still limited. The CCK-8 assay is used to evaluate the viability of NSCLC cells. Downregulation of HDAC1 by shRNA. The TUNEL assay was used to evaluate the role of HDAC1 in NSCLC apoptosis. To evaluate the role of HDAC1 in NSCLC cells migration, the Boyden chamber transwell assay and wound healing assay were used. To evaluate the cells invasion, the matrigel precoated Transwell assay was used. Enzyme-linked immunosorbent assays (ELISAs) were used to detect the level of vascular endothelial growth factor (VEGF) and IL-8 in NSCLC. To investigate the role of HDAC1 in angiogenesis, the tube formation assay was investigated. In this study, we showed that HDAC1 expression was elevated in NSCLC lines compared to that in normal liver cells LO2. Furthermore, downregulation of HDAC1 inhibited cell proliferation, prevented cell migration, decreased cell invasion, reduced tumor angiogenesis and induced cell apoptosis. In summary, HDAC1 may be regarded as a potential indicator for NSCLC patient treatment.
Acknowledgments
This study was supported by the Joint Program of Yunnan Province and Kunming Medical University [No. 2017FE467 (-117), 2017FE468(-109)] and the Grant for Medical and Health Unit with research institutions of Yunnan Province (No. 2017NS256).
Conflict of interest statement: The authors declare no conflict of interest.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Reviews
- Hodgkin lymphoma cell lines: to separate the wheat from the chaff
- The AGO proteins: an overview
- Research Articles/Short Communications
- Protein Structure and Function
- Structural changes at the myrtenol backbone reverse its positive allosteric potential into inhibitory GABAA receptor modulation
- The two major glucokinase isoforms show conserved functionality in β-cells despite different subcellular distribution
- Functional characterization of the mouse Serpina1 paralog DOM-7
- Cell Biology and Signaling
- CD45RO regulates the HIV-1 gp120-mediated apoptosis of T cells by activating Lck
- Silencing of MED27 inhibits adrenal cortical carcinogenesis by targeting the Wnt/β-catenin signaling pathway and the epithelial-mesenchymal transition process
- HDAC1 knockdown inhibits invasion and induces apoptosis in non-small cell lung cancer cells
- Hepatitis B virus X protein promotes proliferation of hepatocellular carcinoma cells by upregulating miR-181b by targeting ING5
Articles in the same Issue
- Frontmatter
- Reviews
- Hodgkin lymphoma cell lines: to separate the wheat from the chaff
- The AGO proteins: an overview
- Research Articles/Short Communications
- Protein Structure and Function
- Structural changes at the myrtenol backbone reverse its positive allosteric potential into inhibitory GABAA receptor modulation
- The two major glucokinase isoforms show conserved functionality in β-cells despite different subcellular distribution
- Functional characterization of the mouse Serpina1 paralog DOM-7
- Cell Biology and Signaling
- CD45RO regulates the HIV-1 gp120-mediated apoptosis of T cells by activating Lck
- Silencing of MED27 inhibits adrenal cortical carcinogenesis by targeting the Wnt/β-catenin signaling pathway and the epithelial-mesenchymal transition process
- HDAC1 knockdown inhibits invasion and induces apoptosis in non-small cell lung cancer cells
- Hepatitis B virus X protein promotes proliferation of hepatocellular carcinoma cells by upregulating miR-181b by targeting ING5