Effect of silver nanoparticles on human primary keratinocytes
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Radoslaw Szmyd
Abstract
Silver nanoparticles (AgNPs) have many biological applications in biomedicine, biotechnology and other life sciences. Depending on the size, shape and the type of carrier, AgNPs demonstrate different physical and chemical properties. AgNPs have strong antimicrobial, antiviral and antifungal activity, thus they are used extensively in a range of medical settings, particularly in wound dressings but also in cosmetics. This study was undertaken to examine the potential toxic effects of 15 nm polyvinylpyrrolidone-coated AgNPs on primary normal human epidermal keratinocytes (NHEK). Cells were treated with different concentrations of AgNPs and then cell viability, metabolic activity and other biological and biochemical aspects of keratinocytes functioning were studied. We observed that AgNPs decrease keratinocyte viability, metabolism and also proliferatory and migratory potential of these cells. Moreover, longer exposure resulted in activation of caspase 3/7 and DNA damage. Our studies show for the first time, that AgNPs may present possible danger for primary keratinocytes, concerning activation of genotoxic and cytotoxic processes depending on the concentration.
©2012 by Walter de Gruyter Berlin Boston
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- Masthead
- Masthead
- Reviews
- Exosomes: the ideal nanovectors for biodelivery
- Biological applications of the electrochemical sensing of nitric oxide: fundamentals and recent developments
- Minireview
- Cerebral cavernous malformation is a vascular disease associated with activated RhoA signaling
- Research Articles/Short Communications
- Protein Structure and Function
- Diversity, abundance, and sex-specific expression of chemosensory proteins in the reproductive organs of the locust Locusta migratoria manilensis
- Structure of the Ca2+-saturated C-terminal domain of scallop troponin C in complex with a troponin I fragment
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- Cell Biology and Signaling
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- Effect of silver nanoparticles on human primary keratinocytes
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- Proteolysis
- Activation profiles of human kallikrein-related peptidases by matrix metalloproteinases
