Preliminary evaluation of a new flow cytometry method for the routine hematology workflow
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Michela Seghezzi
Abstract
Background
In a generalist laboratory, the integration of the data obtained from hematology analyzers (HAs) with those from multiparametric flow cytometry (FMC) could increase the specificity and sensitivity of first level screening to identify the pathological samples. The aim of this study was to perform a preliminary evaluation of a new simple hybrid method (HM). The method was obtained by integration between HAs reagents into FCM, with a basic monoclonal antibodies panel for the leukocytes differential count.
Methods
Eighty-one peripheral blood samples, collected in K3EDTA tubes, were analyzed by XN-module, and CyFlow Space System, using both standard MoAbs and HM method analysis, and with the optical microscopy (OM). Within-run imprecision was carried out using normal samples, the carryover was evaluated, data comparison was performed with Passing-Bablok regression and Bland-Altman plots.
Results
The within-run imprecision of HM methods ranged between 1.4% for neutrophils (NE) and 10.1% for monocytes (MO) always equal or lower to the OM. The comparison between HM methods vs. OM shows Passing-Bablok regression slopes comprised between 0.83 for lymphocyte (LY) and 1.14 for MO, whilst the intercepts ranged between −0.18 for NE and 0.25 for LY. Bland-Altman relative bias was comprised between −12.43% for NE, and 19.77% for eosinophils. In all 11 pathological samples the agreement between the methods was 100%.
Conclusions
The new hybrid method generates a leukocytes differential count suitable for routine clinical use and it is also useful for identifying morphological abnormalities with a reduction in cost and improvement of screening for first level hematology workflow.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: The study was financially supported by Sysmex Corporation and Sysmex America Inc.
Employment or leadership: Dr. Ramon Simon-Lopez is the Consultant Medical Director of Sysmex Corporation and Sysmex America Inc. Dr. Mari Kono is Researcher of Scientific Affairs of Sysmex Corporation and Ezio Tirloni is a Specialist of Product and Application of Sysmex Partec Italia.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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© 2019 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Editorial
- Blood biomarkers in neurology: “a call to arms” for laboratory professionals
- Reviews
- Diagnostic accuracy of glycated hemoglobin for gestational diabetes mellitus: a systematic review and meta-analysis
- Laboratory medicine: health evaluation in elite athletes
- Prostate cancer screening: guidelines review and laboratory issues
- Opinion Papers
- Extra-analytical sources of uncertainty: which ones really matter?
- Benefits and harms of wellness initiatives
- Genetics and Molecular Diagnostics
- Analytical and clinical validation of a novel amplicon-based NGS assay for the evaluation of circulating tumor DNA in metastatic colorectal cancer patients
- General Clinical Chemistry and Laboratory Medicine
- Pre-analytical practices for routine coagulation tests in European laboratories. A collaborative study from the European Organisation for External Quality Assurance Providers in Laboratory Medicine (EQALM)
- Preanalytical robustness of blood collection tubes with RNA stabilizers
- Continual improvement of the pre-analytical process in a public health laboratory with quality indicators-based risk management
- Comparison of six commercial serum exosome isolation methods suitable for clinical laboratories. Effect in cytokine analysis
- A multicenter study to evaluate harmonization of assays for N-terminal propeptide of type I procollagen (PINP): a report from the IFCC-IOF Joint Committee for Bone Metabolism
- Correlations between serum and CSF pNfH levels in ALS, FTD and controls: a comparison of three analytical approaches
- Dynamics of extracellular matrix proteins in cerebrospinal fluid and serum and their relation to clinical outcome in human traumatic brain injury
- Free light chains in the cerebrospinal fluid. Comparison of different methods to determine intrathecal synthesis
- Reference Values and Biological Variations
- Reference interval by the indirect approach of serum thyrotropin (TSH) in a Mediterranean adult population and the association with age and gender
- Next-generation reference intervals for pediatric hematology
- Hematology and Coagulation
- Preliminary evaluation of a new flow cytometry method for the routine hematology workflow
- Diabetes
- Trueness assessment of HbA1c routine assays: are processed EQA materials up to the job?
- Infectious Diseases
- Utility of procalcitonin for differentiating cryptogenic organising pneumonia from community-acquired pneumonia
- A high C-reactive protein/procalcitonin ratio predicts Mycoplasma pneumoniae infection
- Letters to the Editor
- Evaluation of reference change values for a hs-cTnI immunoassay using both plasma samples of healthy subjects and patients and quality control samples
- Outlier removal methods for skewed data: impact on age-specific high-sensitive cardiac troponin T 99th percentiles
- Comparison of precision and operational performances across six immunochemistry analyzers
- Evaluation of the possible interference of abiraterone therapy on testosterone immunoassay
- Erroneous thyroid and steroid hormones profile due to anti-streptavidin antibodies
- Reference values for 24,25-dihydroxyvitamin D and the 25-hydroxyvitamin D/24,25-dihydroxyvitamin D ratio
- Pre-analytical error in a hematology laboratory: an avoidable cause of compromised quality in reporting
- Stability of tubular damage markers epidermal growth factor and heparin-binding EGF-like growth factor in urine
- Blood from heparin tubes is an acceptable alternative to assess hematocrit determination
