Laccase-catalysed functionalisation of TMP with tyramine
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Stina Grönqvist
Abstract
Modified wood fibres open new perspectives to create value-added products based on renewable raw materials. An interesting option is the targeted modification of fibre surfaces by oxidative enzymes. This two-stage functionalisation method consists of enzymatic activation of fibre surfaces followed by addition of radicalised compounds reacting preferentially by radical coupling. In this work, the activation of bleached and unbleached softwood TMPs with laccase isolated from Trametes hirsuta was studied. The formation and stability of the radicals were studied by EPR spectroscopy. The reaction of the radicals with 3-hydroxytyramine hydrochloride and the type of chemical linkages were investigated. EPR, ESCA and FTIR spectroscopy were used for analysis. Bleached TMP was radicalised more efficiently than unbleached TMP. The radicals were unstable, as 90% of them were quenched within a few hours. Their lifetime was, however, found to be adequately long for performing coupling reactions. Bonding of new compounds to pulps via radical reactions thus seems to be possible.
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©2006 by Walter de Gruyter Berlin New York
Articles in the same Issue
- A report from the 2005 Japanese-European Workshop on “Cellulose and Functional Polysaccharides”
- Determination of substituent distribution of viscoses by GPC
- Direct investigation of the structural properties of tension wood cellulose microfibrils using microbeam X-ray fibre diffraction
- Influence of amide content on the crystal structure of chitin
- Stepwise synthesis of chitooligosaccharides through a transition-state analogue substrate catalyzed by mutants of chitinase A1 from Bacillus circulans WL-12
- Synthesis of plantamajoside, a bioactive dihydroxyphenylethyl glycoside from Plantago major L.
- Diffusion of sulfide into Southern pine (Pinus taeda L.) and sweetgum (Liquidambar styraciflua L.) particles and chips
- Laccase-catalysed functionalisation of TMP with tyramine
- Wood liquefaction by ionic liquids
- Studies on the dehydrogenative polymerizations of monolignol β-glycosides. Part 2: Horseradish peroxidase-catalyzed dehydrogenative polymerization of isoconiferin
- Phenolic compounds in silver birch (Betula pendula Roth) wood
- Analysis of fragrance compositions of precious coniferous woods grown in Taiwan
- Influences of hot pressing temperature and surface structure on VOC emissions from OSB made of Scots pine
- Dimensional stability and photostability of octanoylated wood
- Measurement of the shear modulus of wood by the square-plate twist method
- Dielectric relaxation of water adsorbed on chemically treated woods
- Influences of drying on internal checking of spruce (Picea abies L.) heat-treated at 212°C
- The role of resol fortifiers in latex wood adhesives
- A simple anisotropy correction procedure for acoustic wood tomography
- Evaluating growth strain of Eucalyptus globulus Labill. from SilviScan measurements
- Density assessment of radiata pine: Sampling strategy revisited
Articles in the same Issue
- A report from the 2005 Japanese-European Workshop on “Cellulose and Functional Polysaccharides”
- Determination of substituent distribution of viscoses by GPC
- Direct investigation of the structural properties of tension wood cellulose microfibrils using microbeam X-ray fibre diffraction
- Influence of amide content on the crystal structure of chitin
- Stepwise synthesis of chitooligosaccharides through a transition-state analogue substrate catalyzed by mutants of chitinase A1 from Bacillus circulans WL-12
- Synthesis of plantamajoside, a bioactive dihydroxyphenylethyl glycoside from Plantago major L.
- Diffusion of sulfide into Southern pine (Pinus taeda L.) and sweetgum (Liquidambar styraciflua L.) particles and chips
- Laccase-catalysed functionalisation of TMP with tyramine
- Wood liquefaction by ionic liquids
- Studies on the dehydrogenative polymerizations of monolignol β-glycosides. Part 2: Horseradish peroxidase-catalyzed dehydrogenative polymerization of isoconiferin
- Phenolic compounds in silver birch (Betula pendula Roth) wood
- Analysis of fragrance compositions of precious coniferous woods grown in Taiwan
- Influences of hot pressing temperature and surface structure on VOC emissions from OSB made of Scots pine
- Dimensional stability and photostability of octanoylated wood
- Measurement of the shear modulus of wood by the square-plate twist method
- Dielectric relaxation of water adsorbed on chemically treated woods
- Influences of drying on internal checking of spruce (Picea abies L.) heat-treated at 212°C
- The role of resol fortifiers in latex wood adhesives
- A simple anisotropy correction procedure for acoustic wood tomography
- Evaluating growth strain of Eucalyptus globulus Labill. from SilviScan measurements
- Density assessment of radiata pine: Sampling strategy revisited
