Spectrophotometric determination of microamounts of quercetin based on its complexation with copper(II)
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D. Kostić
,
G. Miletić
Abstract
The complexation process of the transition metal Cu(II) with quercetin was studied. The investigation was conducted spectrophotometrically in ethanol at the maximum absorption wavelength of 458.5 nm. Cu(II)—quercetin complex composition (1: 1) was determined using the Job, Harvey—Manning, and mole ratio methods. Complex stability constant was calculated by the Job and mole ratio methods and the respective logarithm values were 7.53 ± 0.25 and 7.44 ± 0.03. A new method for quantitative determination of the quercetin content in solution was developed in this work. At the optimal conditions quercetin was determined in concentrations ranging from 0.202 to 1.006 µg cm−3 with relative standard error of 2.5 % to 5.5 %. The lower detection limit was 0.067 µg cm−3. The method was found very accurate, reproducible, and sensitive, capable to determine microamounts of quercetin in pharmaceutical preparations.
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© 2007 Institute of Chemistry, Slovak Academy of Sciences
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Artikel in diesem Heft
- Spectrophotometric determination of microamounts of quercetin based on its complexation with copper(II)
- Oxygen evolution on Ti/Co3O4-coated electrodes in alkaline solution
- The zeta potential of kaolin suspensions measured by electrophoresis and electroacoustics
- Fluidization behavior of oil-contaminated sand
- Software sensors for monitoring of a solid waste composting process
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- Thermodynamic possibilities and constraints of pure hydrogen production by a chromium, nickel, and manganese-based chemical looping process at lower temperatures
- Death kinetics of Escherichia coli in goat milk and Bacillus licheniformis in cloudberry jam treated by ohmic heating
- Topochemical models for anti-HIV activity of 1-alkoxy-5-alkyl-6-(arylthio)uracils
- Acidity, lipophilicity, solubility, absorption, and polar surface area of some ACE inhibitors
- Silver as anode in cryolite—alumina-based melts
- Multicomponent facile synthesis of novel dihydroazolopyrimidinyl carbamides
- Z. Platková, M. Polakovič, V. Štefuca, M. Vandáková, and M. Antošová: Selection of Carrier for Immobilization of Fructosyltransferase from Aureobasidium pullulans
