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Effects of ionic strength on the coordination of Eu(III) and Cm(III) to a Gram-negative bacterium, Paracoccus denitrificans
-
Takuo Ozaki
Veröffentlicht/Copyright:
25. September 2009
We studied the effect of ionic strength on the interactions of Europium(III) and Curium(III) with a Gram-negative bacterium Paracoccus denitrificans . Bacterial cells grown in 0.5-, 3.5-, and 5.0% NaCl were used in adsorption experiments and laser experiments that were performed at the same ionic strengths as those in the original growth media. The distribution ratio (logKd) for Eu(III) and Cm(III) was determined at pHs 3−5. To elucidate the coordination environment of Eu(III) adsorbed on P. denitrificans, we estimated the number of water molecules in the inner sphere and strength of the ligand field by time-resolved laser-induced fluorescence spectroscopy (TRLFS) at pHs 4−6. The logKd of Eu(III) and Cm(III) increased with an increase of pH at all ionic strengths because there was less competition for ligands in cells with H+ at higher pHs, wherein less H+ was present in solution: cation adsorption generally occurs through an exchange with H+ on the functional groups of coordination sites. No significant differences were observed in the logKd of Eu(III) and Cm(III) at each pH in 0.5-, 3.5-, and 5.0% NaCl solutions, though competition for ligands with Na+ would be expected to increase at higher NaCl concentrations. The logKd of Eu(III) was almost equivalent to that of Cm(III) under all the experimental conditions. TRLFS showed that the coordination environments of Eu(III) did not differ from each other at 0.5-, 3.5-, and 5.0% NaCl at pHs 4−6. TRLFS also showed that the characteristic of the coordination environment of Eu(III) on P. denitrificans was similar to that on a halophile, Nesterenkonia halobia, while it significantly differed from that on a non-halophile, Pseudomonas putida . These findings indicate that the number of coordination sites for Eu(III) on P. denitrificans, whose cell surface may have similar structures to that of halophiles, increased with increasing ionic strength, though their structure remained unchanged.
Keywords: Ionic strength; Paracoccus denitrificans; Europium(III); Curium(III); Coordination environment; Time-resolved laser-induced fluorescence spectroscopy (TRLFS)
Received: 2005-10-5
Accepted: 2006-3-26
Published Online: 2009-9-25
Published in Print: 2006-11-1
© Oldenbourg Wissenschaftsverlag
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Schlagwörter für diesen Artikel
Ionic strength;
Paracoccus denitrificans;
Europium(III);
Curium(III);
Coordination environment;
Time-resolved laser-induced fluorescence spectroscopy (TRLFS)
Artikel in diesem Heft
- Preface Migration 2005
- Solubility measurement of zirconium(IV) hydrous oxide
- Solubility of ThO2·xH2O(am) and the formation of ternary Th(IV) hydroxide-carbonate complexes in NaHCO3-Na2CO3 solutions containing 0−4 M NaCl
- Ion interaction (SIT) coefficients for the Th4+ ion and trace activity coefficients in NaClO4, NaNO3 and NaCl solution determined by solvent extraction with TBP
- Leaching of neptunium from garnet- and murataite-based ceramics
- Coprecipitation of thorium and lanthanum with UO2+x(s) as host phase
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- Np(V)O2+ sorption on hydroxyapatite-effect of calcium and phosphate anions
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