Fabrication of palm carotene-based emulsion stabilized by rice protein isolate-flaxseed gum complex
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Hew Weng Choy
Abstract
The aim of this study was to develop a stable palm carotene-based emulsion using the rice protein isolate-flaxseed gum complex (RPI-FG) with different ratios (3:1, 5:1, 7:1, 9:1, 11:1). RPI achieved highest solubility at pH 12. Meanwhile, RPI-FG complex shows low turbidity value from pH 6 to 12 for all ratios. Emulsion with ratio 7:1 exhibited 33 % droplet size increment throughout 7 days storage and evenly distributed based on the microstructure images. For creaming index (CI), all emulsion shows 0 % separation on day zero except emulsion with ratio 11:1 (1.90 %) and lowest CI on day 7 was 9.05 % in ratio 3:1. Furthermore, emulsion activity index and emulsion stability index of the emulsions decreased significantly with the reduced of FG in the formulation. These results suggested that carotene-based emulsion fabricated using RPI-FG complex with ratio of 7:1 exhibited the highest emulsion stability and could be further applied in aqueous food systems.
Funding source: Ministry of Higher Education, Malaysia
Award Identifier / Grant number: FRGS/1/2020/WAB04/UPM/01/4
Acknowledgments
We would like to express our gratitude to Anhui Shunxin Shengyuan Biological Food Co. Ltd, Qingdao Dehui Halobios Science and Technology Co Ltd, and ExcelVite Sdn. Bhd for kindly providing the rice protein isolate, flaxseed gum and natural palm-based carotene concentrate used in this research.
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Research ethics: Not applicable.
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Informed consent: Not applicable.
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Author contributions: The authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: The authors state no conflict of interest.
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Research funding: The work was supported by the Fundamental Research Grant Scheme (FRGS/1/2020/WAB04/UPM/01/4) from the Ministry of Higher Education, Malaysia.
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Data availability: Not applicable.
References
1. Liang, R, Huang, Q, Ma, J, Shoemaker, CF, Zhong, F. Effect of relative humidity on the store stability of spray-dried beta-carotene nanoemulsions. Food Hydrocolloids 2018;33:225–33. https://doi.org/10.1016/j.foodhyd.2013.03.015.Suche in Google Scholar
2. Mehmood, T, Ahmed, A, Ahmad, A, Ahmad, MS, Sandhu, MA. Optimization of mixed surfactants-based β-carotene nanoemulsions using response surface methodology: an ultrasonic homogenization approach. Food Chem 2018;253:179–84. https://doi.org/10.1016/j.foodchem.2018.01.136.Suche in Google Scholar PubMed
3. Romero, A, Beaumal, V, David-Briand, E, Cordobes, F, Guerrero, A, Anton, M. Interfacial and emulsifying behaviour of rice protein concentrate. Food Hydrocolloids 2012;29:1–8. https://doi.org/10.1016/j.foodhyd.2012.01.013.Suche in Google Scholar
4. Pinciroli, M, Vidal, AA, Anon, MC, Martínez, EN. Comparison between protein functional properties of two rice cultivars. LWT 2009;42:1605–10. https://doi.org/10.1016/j.lwt.2009.06.003.Suche in Google Scholar
5. El-Sayed, MA, Youssef, MM, Adel-Shehata, A, El-Mahdy, AR. Extractability and functionality of rice proteins and their application as meat extenders. Food Chem 1986;20:79–83. https://doi.org/10.1016/03088146(86)90169-x.Suche in Google Scholar
6. Dickinson, E. Hydrocolloids at interfaces and the influence on the properties of dispersed systems. Food Hydrocolloids 2003;17:25–39. https://doi.org/10.1016/s0268-005x(01)00120-5.Suche in Google Scholar
7. Liu, J, Shim, YY, Poth, AG, Reaney, MJT. Conlinin in flaxseed (Linum usitatissimum L.) gum and its contribution to emulsification properties. Food Hydrocolloids 2016;52:963–71. https://doi.org/10.1016/j.foodhyd.2015.09.001.Suche in Google Scholar
8. Khalloufi, S, Corredig, M, Goff, HD, Alexander, M. Flaxseed gums and their adsorption on whey protein-stabilized oil-in-water emulsions. Food Hydrocolloids 2009;23:611–18. https://doi.org/10.1016/j.foodhyd.2008.04.004.Suche in Google Scholar
9. Wang, Y, Wang, LJ, Li, D, Xue, J, Mao, ZH. Effects of drying methods on rheological properties of flaxseed gum. Carbohydr Polym 2009;78:213–19. https://doi.org/10.1016/j.carbpol.2009.03.025.Suche in Google Scholar
10. Evans, M, Ratcliffe, I, Williams, PA. Emulsion stabilisation using polysaccharide protein complexes. Curr Opin Colloid Interface Sci 2013;18:272–82. https://doi.org/10.1016/j.cocis.2013.04.004.Suche in Google Scholar
11. Matemu, AO, Kayahara, H, Murasawa, H, Nakamura, S. Importance of size and charge of carbohydrate chains in the preparation of functional glycoproteins with excellent emulsifying properties from tofu whey. Food Chem 2009;114:1328–34. https://doi.org/10.1016/j.foodchem.2008.11.011.Suche in Google Scholar
12. Zinoviadou, KG, Scholten, E, Moschakis, T, Biliaderis, CG. Properties of emulsions stabilised by sodium caseinate–chitosan complexes. Int Dairy J 2012;26:94–101. https://doi.org/10.1016/j.idairyj.2012.01.007.Suche in Google Scholar
13. Weinbreck, F, de Vries, R, Schrooyen, P, de Kruif, CG. Complex coacervation of whey proteins and gum Arabic. Biomacromolecules 2003;4:293–303. https://doi.org/10.1021/bm025667n.Suche in Google Scholar PubMed
14. McClements, DJ. Food emulsions: principles, practices, and techniques, 3rd ed. Boca Raton, FL: CRC Press; 2015:714 p.10.1201/b18868Suche in Google Scholar
15. Kruger, NJ. Basic protein and peptide protocols: the Bradford method for protein quantitation. Totowa, NJ: Humana Press; 1994, vol 32.Suche in Google Scholar
16. Chang, HW, Tan, TB, Tan, PY, Faridah, A, Lai, OM, Wang, Y, et al.. Physical properties and stability evaluation of fish oil-in-water emulsions stabilized using thiol-modified β-lactoglobulin fibrils chitosan complex. Food Res Int 2018;105:482–91. https://doi.org/10.1016/j.foodres.2017.11.034.Suche in Google Scholar PubMed
17. Khor, YP, Koh, SP, Kamariah, L, Chong, GH, Tan, CP. Stability assessment of virgin coconut oil-based emulsion products. J Am Oil Chem Soc 2018;95:1329–39. https://doi.org/10.1002/aocs.12109.Suche in Google Scholar
18. Gua, Q, Mu, TH. Emulsifying properties of sweet potatoes protein: effect of protein concentration and oil volume fraction. Food Hydrocolloids 2010;25:98–106. https://doi.org/10.1016/j.foodhyd.2010.05.011.Suche in Google Scholar
19. Cao, XH, Wen, HB, Li, CJ, Gu, ZX. Differences in functional properties and biochemical characteristics of congenetic rice proteins. J Cereal Sci 2009;50:184–9. https://doi.org/10.1016/j.jcs.2009.04.009.Suche in Google Scholar
20. Karunaratne, DN. The complex world of polysaccharides: polysaccharide protein interactions and their relevance in food colloids. London, UK: Intech Open; 2012.Suche in Google Scholar
21. Zhang, LT, Pan, Z, Shen, KQ, Cai, XH, Zheng, BD, Miao, S. Influence of ultrasound-assisted alkali treatment on the structural properties and functionalities of rice protein. J Cereal Sci 2018;79:204–9. https://doi.org/10.1016/j.jcs.2017.10.013.Suche in Google Scholar
22. Wang, L, Wu, M, Liu, HM. Emulsifying and physicochemical properties of soy hullhemicelluloses-soy protein isolate conjugates. Carbohydr Polym 2017;163:181–90. https://doi.org/10.1016/j.carbpol.2017.01.069.Suche in Google Scholar PubMed
23. Raoufi, N, Fang, YP, Kadkhodaee, R, Phillips, GO, Najafi, MN. Changes in turbidity, zeta potential and precipitation yield induced by Persian gum-whey protein isolate interactions during acidification. J Food Process Preserv 2016;41:1–8. https://doi.org/10.1111/jfpp.12975.Suche in Google Scholar
24. McClements, DJ. Protein-stabilized emulsions. Curr Opin Colloid Interface Sci 2004;9:305–13. https://doi.org/10.1016/j.cocis.2004.09.003.Suche in Google Scholar
25. Tan, CP, Nakajima, M. β-Carotene nanodispersions: preparation, characterization and stability evaluation. Food Chem 2005;92:661–71. https://doi.org/10.1016/j.foodchem.2004.08.044.Suche in Google Scholar
26. Alicia, ML, Medina, WT, Park, DJ, Aguilera, JM. Properties of microparticles from a whey protein isolate/alginate emulsion gel. Food Sci Technol Int 2018;24:414–23. https://doi.org/10.1177/1082013218762210.Suche in Google Scholar PubMed
27. Felix, M, Isurralde, N, Romero, A, Guerrero, A. Influence of pH value on microstructure of oil-in-water emulsions stabilized by chickpea protein flour. Food Sci Technol Int 2018;24:555–63. https://doi.org/10.1177/1082013218774707.Suche in Google Scholar PubMed
28. Guzey, D, McClements, DJ. Formation, stability and properties of multilayer emulsions for application in the food industry. Adv Colloid Interface Sci 2006;128–130:227–48. https://doi.org/10.1016/j.cis.2006.11.021.Suche in Google Scholar PubMed
29. Sun, M, Li, X, McClements, DJ, Xiao, M, Chen, HJ, Zhou, Q, et al.. Reducing off-flavors in plant-based omega-3 oil emulsions using interfacial engineering: coating algae oil droplets with pea protein/flaxseed gum. Food Hydrocolloids 2021;122:107069. https://doi.org/10.1016/j.foodhyd.2021.107069.Suche in Google Scholar
30. Seid, MJ, He, YH, Bhesh, B. Optimization of nano-emulsions production by microfluidization. Eur Food Res Tech 2007;225:733–41. https://doi.org/10.1007/s00217-006-0476-9.Suche in Google Scholar
31. Klongdee, S, Thongngam, M, Klinkesorn, U. Rheology and microstructure of lecithin-stabilized tuna oil emulsions containing chitosan of varying concentration and molecular size. Food Biophys 2012;7:155–62. https://doi.org/10.1007/s11483-012-9253-4.Suche in Google Scholar
32. Georgiadis, N, Ritzoulis, C, Sioura, G, Kornezou, P, Vasiliadou, C, Tsioptsias, C. Contribution of okra extracts to the stability and rheology of oil-in- water emulsions. Food Hydrocolloids 2011;25:991–9. https://doi.org/10.1016/j.foodhyd.2010.09.014.Suche in Google Scholar
33. Nystrom, B, Kjoniksen, AL, Iversen, C. Characterization of association phenomena in aqueous systems of chitosan of different hydrophobicity. Adv Colloid Interface Sci 1999;79:81–103. https://doi.org/10.1016/s0001-8686(98)00069-4.Suche in Google Scholar
34. Sun, LH, Yu, F, Wang, YY, Lv, SW, He, LY. Effects of ultrasound extraction on the physicochemical and emulsifying properties of rice bran protein. Int J Food Eng 2021;17:327–35. https://doi.org/10.1515/ijfe-2019-0115.Suche in Google Scholar
35. Piorkowski, DT, McClements, DJ. Beverage emulsions: recent developments in formulation, production, and applications. Food Hydrocolloids 2014;42:5–41. https://doi.org/10.1016/j.foodhyd.2013.07.009.Suche in Google Scholar
36. Chuah, AM, Kuroiwa, T, Kobayashi, I, Nakajima, M. Effect of chitosan on the stability and properties of modified lecithin stabilized oil-in-water monodisperse emulsion prepared by microchannel emulsification. Food Hydrocolloids 2009;23:600–10. https://doi.org/10.1016/j.foodhyd.2008.03.014.Suche in Google Scholar
37. Li, P, Zhang, W, Han, X, Liu, J, Liu, Y, Gasmalla, MAA, et al.. Demulsification of oil-rich emulsion and characterization of protein hydrolysates from peanut cream emulsion of aqueous extraction processing. J Food Eng 2017;204:64–72. https://doi.org/10.1016/j.jfoodeng.2017.02.009.Suche in Google Scholar
38. Pugnaloni, LA, Dickinson, E, Ettelaie, R, Mackie, AR, Wilde, PJ. Competitive adsorption of proteins and low-molecular-weight surfactants: computer simulation and microscopic imaging. Adv Colloid Interface Sci 2004;107:27–49. https://doi.org/10.1016/s0001-8686(03)00128-3.Suche in Google Scholar
39. Wang, S, Shao, G, Yang, J, Zhao, H, Qu, D, Zhang, D, et al.. Contribution of soybean polysaccharides in digestion of oil-in-water emulsion-based delivery system in an in vitro gastric environment. Food Sci Technol Int 2020;26:444–52. https://doi.org/10.1177/1082013219894145.Suche in Google Scholar PubMed
40. Sun, CY, Hu, YQ, Yu, XT, Zhu, ZJ, Hao, S, Du, XF. Morphological, structural and physicochemical properties of rice starch nanoparticles prepared via ultra-high-pressure homogenization. Int J Food Eng 2021;17:981–8. https://doi.org/10.1515/ijfe-2021-0186.Suche in Google Scholar
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Artikel in diesem Heft
- Frontmatter
- Critical Review
- Preparation of solution blow spinning nanofibers and its application in the food field: a review
- Articles
- Structural identification, stability and combination mechanism of calcium-chelating wheat gluten peptides
- Increased reactive carboxyl and free alfa-amino groups from fish type I collagen peptides by Alcalase® hydrolysis exhibit higher antibacterial and antioxidant activities
- Comparative study on enzyme activity, microstructure, drying kinetics, and physicochemical properties of apple slices affected by microwave and steam blanching
- Acerola byproducts microencapsulated by spray and freeze-drying: the effect of carrier agent and drying method on the production of bioactive powder
- Study of the textural properties of non-ionic resins and their influence on polyphenol adsorption and desorption
- Fabrication of palm carotene-based emulsion stabilized by rice protein isolate-flaxseed gum complex
- Physicochemical properties, the digestible and resistant starch content of chestnut flour with different particle sizes
Artikel in diesem Heft
- Frontmatter
- Critical Review
- Preparation of solution blow spinning nanofibers and its application in the food field: a review
- Articles
- Structural identification, stability and combination mechanism of calcium-chelating wheat gluten peptides
- Increased reactive carboxyl and free alfa-amino groups from fish type I collagen peptides by Alcalase® hydrolysis exhibit higher antibacterial and antioxidant activities
- Comparative study on enzyme activity, microstructure, drying kinetics, and physicochemical properties of apple slices affected by microwave and steam blanching
- Acerola byproducts microencapsulated by spray and freeze-drying: the effect of carrier agent and drying method on the production of bioactive powder
- Study of the textural properties of non-ionic resins and their influence on polyphenol adsorption and desorption
- Fabrication of palm carotene-based emulsion stabilized by rice protein isolate-flaxseed gum complex
- Physicochemical properties, the digestible and resistant starch content of chestnut flour with different particle sizes